LOCUS Exported 5172 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Nucleus, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-CENPA-C-22 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5172) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 5172) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5172 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 84..387 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 388..591 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 542..562 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 630..639 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 636..1352 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(681..702) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(942..961) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1289..1310 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 1983..2104 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2020..2039) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2074..2093 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2111..2566) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2248..2269) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2460..2479 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2593..2697 /gene="bla" /label=AmpR promoter promoter 2699..3056 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2722..2742) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2907..3042 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2969..2988 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3091..3885 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3145..3164) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3755..3774 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4073..4092) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4117..4164 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4493..5081 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4982..5001 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt 61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga 121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca 181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca 241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg 301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 361 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt 421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt 481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg 541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg 601 aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt 661 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 721 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 781 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg 841 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 901 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 961 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 1021 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 1081 acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc 1141 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 1201 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga 1261 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 1321 ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt 1381 cgaattctag cagttctaga gtaagcagca gagtaagtgg cccgcgccgc cggagccgaa 1441 agcccgaggc cccgaggagg cgcagcccga gcccgacccc gacccccggc ccctcccggc 1501 ggggcccctc cttaggcgct tcctcccatc aacacagtcg gcggagacaa ggttggctaa 1561 aggagatccg aaagcttcag aagagcacac acctcttgat aaggaagctg cccttcagcc 1621 gcctggcaag agaaatatgt gttaaattca ctcgtggtgt ggacttcaat tggcaagccc 1681 aggccctatt ggccctacaa gaggcagcag aagcatttct agttcatctc tttgaggacg 1741 cctatctcct caccttacat gcaggccgag ttactctctt cccaaaggat gtgcaactgg 1801 cccggaggat ccggggcctt gaggagggac tcggctaagg taccgcgggc ccgggatcca 1861 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt 1921 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg 1981 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 2041 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 2101 cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 2161 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 2221 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 2281 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 2341 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 2401 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 2461 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 2521 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt 2581 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 2641 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct 2701 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 2761 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 2821 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 2881 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 2941 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 3001 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc 3061 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 3121 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 3181 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 3241 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 3301 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 3361 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 3421 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 3481 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 3541 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 3601 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 3661 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 3721 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 3781 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 3841 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt 3901 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 3961 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 4021 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac 4081 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa 4141 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 4201 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac 4261 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc 4321 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat 4381 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 4441 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 4501 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 4561 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 4621 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 4681 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 4741 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 4801 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 4861 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 4921 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 4981 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 5041 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 5101 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 5161 ctgattctgt gg //