LOCUS       Exported                4299 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-SLC17A6_STOP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4299)
  TITLE     RESOLUTE consortium SLC collection
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2424..2523)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2540..2559)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2541..2559)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2564..2580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2564..2580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2682..2701)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2693..3502
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2760..2779)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3649..4237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4138..4157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggaga gcgtgaagca gaggatcctg gcacctggca aggagggcct
      721 gaagaacttc gccggcaagt ccctgggcca gatctacaga gtgctggaga agaagcagga
      781 cacaggcgag accatcgagc tgacagagga tggcaagcca ctggaggtgc ccgagagaaa
      841 ggcccccctg tgcgactgta cctgctttgg cctgcctcgg agatatatca tcgccatcat
      901 gagcggcctg ggcttctgta tctcctttgg catccggtgc aacctgggcg tggccatcgt
      961 ggacatggtg aacaatagca ccatccacag aggcggcaaa gtgatcaagg agaaggccaa
     1021 gttcaattgg gatccagaga cagtgggcat gatccacggc tccttctttt ggggctacat
     1081 catcacccag atccccggcg gctatatcgc ctctaggctg gcagcaaacc gcgtgttcgg
     1141 agcagcaatc ctgctgacat ctaccctgaa tatgctgatc ccaagcgccg caagggtgca
     1201 ctacggatgc gtgatctttg tgagaatcct gcagggcctg gtggagggcg tgacatatcc
     1261 tgcctgccac ggaatctgga gcaagtgggc accacctctg gagcggtcca gactggccac
     1321 cacaagcttc tgtggatcct acgcaggagc cgtgatcgca atgccactgg ccggcatcct
     1381 ggtgcagtat accggctgga gctccgtgtt ctacgtgtat ggcagctttg gcatggtgtg
     1441 gtacatgttt tggctgctgg tgtcctatga gtctcctgcc aagcacccaa caatcaccga
     1501 tgaggagagg cgctacatcg aggagtctat cggagagagc gccaacctgc tgggagcaat
     1561 ggagaagttc aagacaccat ggaggaagtt ctttacctcc atgcccgtgt atgccatcat
     1621 cgtggccaat ttctgtcgct cttggacctt ctacctgctg ctgatcagcc agcccgccta
     1681 ttttgaggag gtgttcggct ttgagatctc taaagtgggc atgctgagcg ccgtgcctca
     1741 cctggtcatg accatcatcg tgccaatcgg cggccagatc gccgacttcc tgaggtccaa
     1801 gcagatcctg tctaccacaa ccgtgcgcaa gatcatgaac tgcggcggct ttggcatgga
     1861 ggccacactg ctgctggtgg tgggatactc ccacaccagg ggagtggcca tctctttcct
     1921 ggtgctggcc gtgggcttca gcggctttgc catctccggc tttaacgtga atcacctgga
     1981 tatcgcccct cgctatgcct ccatcctgat gggcatctct aacggcgtgg gcacactgtc
     2041 tggcatggtg tgcccaatca tcgtgggcgc catgaccaag aataagagcc gggaggagtg
     2101 gcagtacgtg ttcctgatcg ccgccctggt gcactacgga ggcgtgatct tctatgccat
     2161 ctttgcatcc ggagagaagc agccatgggc agaccctgag gagacctctg aggagaagtg
     2221 cggctttatc cacgaggacg agctggatga ggagacaggc gatatcaccc agaactacat
     2281 caattatggc acaaccaaga gctacggagc aacaacccag gcaaatggag gatggccatc
     2341 cggatgggag aagaaggagg agttcgtgca gggcgaggtg caggactctc acagctacaa
     2401 ggaccgggtg gattattcct aagacccagc tttcttgtac aaagttggca ttataagaaa
     2461 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     2521 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     2581 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaataatatc
     2641 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     2701 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     2761 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     2821 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     2881 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     2941 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
     3001 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     3061 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     3121 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     3181 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     3241 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     3301 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     3361 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     3421 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     3481 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     3541 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
     3601 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3661 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3721 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3781 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3841 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3901 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3961 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4021 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4081 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4141 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4201 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4261 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
