LOCUS Exported 2982 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence. . ACCESSION . VERSION . KEYWORDS pDONR221-SLC31A2_STOP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2982) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 2982) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2982 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1107..1206) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1223..1242) /label=T7 /note="T7 promoter, forward primer" promoter complement(1224..1242) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1247..1263) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1247..1263) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1365..1384) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1376..2185 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1443..1462) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2332..2920 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2821..2840 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggcca tgcacttcat cttttccgac accgccgtgc tgctgttcga 721 tttttggtcc gtgcactctc cagcaggaat ggcactgtct gtgctggtgc tgctgctgct 781 ggccgtgctg tacgagggca tcaaggtcgg caaggccaag ctgctgaacc aggtgctggt 841 gaatctgccc accagcatct cccagcagac catcgcagag acagacggcg atagcgccgg 901 atctgacagc ttccctgtgg gacggacaca ccacagatgg tacctgtgcc actttggcca 961 gtctctgatc cacgtgatcc aggtggtcat cggctacttc atcatgctgg ccgtgatgag 1021 ctataacacc tggatctttc tgggagtggt gctgggaagc gccgtgggat actatctggc 1081 ctatcccctg ctgtccacag cctaagaccc agctttcttg tacaaagttg gcattataag 1141 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat 1201 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc 1261 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaataat 1321 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag 1381 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata 1441 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta 1501 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga 1561 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat 1621 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa 1681 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct 1741 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga 1801 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag 1861 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa 1921 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct 1981 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga 2041 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca 2101 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca 2161 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag 2221 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta 2281 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 2341 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 2401 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 2461 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 2521 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 2581 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 2641 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 2701 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 2761 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 2821 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 2881 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 2941 tttacggttc ctggcctttt gctggccttt tgctcacatg tt //