LOCUS       Exported                4683 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-SLCO3A1_STOP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4683)
  TITLE     RESOLUTE consortium SLC collection
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2808..2907)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2924..2943)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2925..2943)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2948..2964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2948..2964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3066..3085)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3077..3886
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3144..3163)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4033..4621
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4522..4541
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgcagg gcaagaagcc aggaggaagc tccggaggag gccggtccgg
      721 cgagctgcag ggcgatgagg cccagagaaa caagaagaag aagaagaagg tgagctgctt
      781 ctctaacatc aagatctttc tggtgtctga gtgtgcactg atgctggcac agggaaccgt
      841 gggagcatat ctggtgtctg tgctgaccac actggagcgg agattcaatc tgcagagcgc
      901 cgacgtgggc gtgatcgcat ctagctttga gatcggcaac ctggccctga tcctgttcgt
      961 gtcttacttt ggagcaaggg gacacaggcc aagactgatc ggatgcggag gaatcgtgat
     1021 ggcactgggc gccctgctga gcgccctgcc cgagttcctg acccaccagt acaagtatga
     1081 ggcaggagag atcagatggg gagcagaggg cagagacgtg tgcgccgcca atggaagcgg
     1141 aggcgacgag ggaccagacc cagatctgat ctgtaggaat cgcacagcca ccaacatgat
     1201 gtatctgctg ctgatcggag cacaggtgct gctgggaatc ggagccaccc ctgtgcagcc
     1261 actgggcgtg tcctatatcg acgatcacgt gaggcgcaag gattcctctc tgtacatcgg
     1321 catcctgttc accatgctgg tgtttggacc tgcatgcggc ttcatcctgg gatccttttg
     1381 tacaaagatc tacgtggacg ccgtgttcat cgatacatct aacctggaca tcaccccaga
     1441 cgatcctagg tggatcggag catggtgggg aggctttctg ctgtgcggcg ccctgctgtt
     1501 ctttagctcc ctgctgatgt tcggctttcc acagagcctg ccacctcact ccgagccagc
     1561 aatggagagc gagcaggcca tgctgtccga gcgggagtat gagagaccta agccaagcaa
     1621 tggcgtgctg aggcacccac tggagccaga ctctagcgcc tcctgtttcc agcagctgcg
     1681 cgtgatccct aaggtgacca agcacctgct gagcaaccca gtgtttacat gcatcatcct
     1741 ggccgcctgt atggagatcg cagtggtggc aggattcgca gcctttctgg gcaagtacct
     1801 ggagcagcag ttcaatctga ccacatcctc tgccaaccag ctgctgggca tgaccgccat
     1861 cccttgcgcc tgtctgggca tctttctggg cggcctgctg gtgaagaagc tgagcctgtc
     1921 cgccctggga gcaatccgga tggccatgct ggtgaatctg gtgtctacag cctgctatgt
     1981 gagcttcctg tttctgggat gtgatacagg accagtggca ggagtgaccg tgccttatgg
     2041 caactctaca gcaccaggaa gcgccctgga cccttacagc ccatgcaaca ataactgcga
     2101 gtgtcagaca gattccttca cccccgtgtg cggagcagac ggaatcacct acctgtctgc
     2161 ctgctttgcc ggctgtaata gcacaaacct gaccggatgc gcatgtctga ccacagtgcc
     2221 agcagagaat gcaaccgtgg tgcctggcaa gtgcccatcc cctggatgtc aggaggcctt
     2281 cctgacattt ctgtgcgtga tgtgcatctg ttccctgatc ggagcaatgg cacagacccc
     2341 atctgtgatc atcctgatcc ggacagtgtc ccctgagctg aagtcttacg ccctgggcgt
     2401 gctgttcctg ctgctgagac tgctgggctt catcccaccc cctctgatct ttggcgccgg
     2461 catcgattcc acatgcctgt tctggtctac cttttgcgga gagcagggag catgcgtgct
     2521 gtatgacaac gtggtgtaca gatatctgta cgtgagcatc gccatcgccc tgaagtcctt
     2581 cgcctttatc ctgtacacca caacctggca gtgtctgagg aagaattata agcggtacat
     2641 caagaaccac gagggcggcc tgtctaccag cgagttcttt gccagcacac tgaccctgga
     2701 taatctggga agggacccag tgccagcaaa ccagacacac cgcaccaagt tcatctacaa
     2761 tctggaggat cacgagtggt gcgagaacat ggagtccgtg ctgtaagacc cagctttctt
     2821 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2881 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2941 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     3001 cattgcacaa gataaaataa tatcatcatg aacaataaaa ctgtctgctt acataaacag
     3061 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     3121 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     3181 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     3241 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     3301 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3361 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3421 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3481 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3541 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3601 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3661 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3721 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3781 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3841 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3901 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3961 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     4021 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4081 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4141 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4201 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4261 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4321 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4381 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4441 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4501 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4561 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4621 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4681 gtt
//
