LOCUS Exported 10691 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning. ACCESSION . VERSION . KEYWORDS pBbdCas9S(-10TC) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10691) AUTHORS Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C TITLE A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi. JOURNAL Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. PUBMED 33257311 REFERENCE 2 (bases 1 to 10691) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10691 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1758..1802 /label=T5 promoter /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 1778..1794 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 1810..1826 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 1845..1856 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 1863..5969 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 6139..6930 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 7053..7641 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7542..7561 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agggctttac aaagttcttt taaaagagaa tttagtaaag ccctaagtct tttaataaaa 61 aattttttgt aaaaaagttg gcaaaaatag tttttgctat atatttatat ataagaaaaa 121 attcaatact caaggagata aaaaatggaa aacttaaaca ataatccaca aaaaattaat 181 caaataaata agcaaactaa ttttattggt tttgaaaatt ttatagaact tgataatcaa 241 gttaatcaaa ctaaagaaaa aagtaaatta agtaagatag gtaagaaatt acccggaata 301 agcagtcaag tctagtgcgg ccggcgcgcc aaataatgcc cgtgtccctg ttgaataggg 361 acacggtttt ttaacaattt ttttattcct aggccttagt ggccggtacc cggggatcct 421 tattactggc cgctttctag cctggatact tgtctggcta gttgcattag agagtcagct 481 agagccctag ggctagctgt ttgtgtattc ggagccagtg tagtttttct ttttactaac 541 gatacgggta ataattgatt gccttttaca gcctgacctt gagacaattg caaaagtctg 601 tccacagagg tttgacctaa taatctaaaa tcttgtttga tagtagtaag tggaggtatg 661 tagcaactag aatcttctgt atcatcataa cccacaactg aaatatcagc cccaactcta 721 agtccacttt cagtaatagc tcgcatagct cctaaagcca tttgatcatt agcaactaac 781 atagcagtag ggacaatgcc ttcatttaac atttgcattg tttgctgaaa tcctgacata 841 gcagaccaat ctccttctct ttctgctatt ggttgtattt gatttcttgt taaatacttg 901 tgccatcctg ctaatctcaa tctggcactt actgaactta agggacctgc aagtagtgca 961 atttgttgat ggcctaatgc aacaaggtgt tcaactccta accttgttcc atcttcgtga 1021 gaaaaaatta tagagtttat tggggtttgg tccgacacat ccaaaaacaa tgctggaaca 1081 ttagtacatg ctgcttcaac tgcaatcgca tcttggtcat caagaggata attaattata 1141 agtccactaa cacgttgagc taataaatta tgtactgctg ccttacaagc ctctacacct 1201 gatctctcaa ccattgatac aacaacgctt gcaccaagtt gatcagctct cgatttaatt 1261 gcggctacaa tttgactagg cgcatgtaat gcaagtgatg atgtggcaac tccaattaaa 1321 agtgattgtt tccccgcaag ttgctgagca acgcgatttg gtatataatt caactctgcc 1381 atagctgctt caaccttctc tcttgttttt gcagatacat gacttgcttg gttcacaact 1441 ctcgaaaccg tttggtagct aaccccggca tattcagcaa catcatacaa agtaacaggt 1501 ttcatggatc attcctccat gataaaattt aaatttctga ctttggcaaa tccttttgcc 1561 tgttaaaaag aatagcacaa aaattaaaaa aaaagaaacg caaatagcaa tagcattaaa 1621 aataattatt attatttatt tctttgatta aaatcaaaag cctttaatgt attttcttga 1681 tctttcatgc gcttaacggt aaaatccaag gtattaagtt caccttccac ttttctctct 1741 ctatcttctc tagaaaatca taaaaaattt atttgctttg tgagcggata acaattataa 1801 cagattcaat tgtgagcgga taacaatttc acacagaatt cattaaagag gagaaattac 1861 atatggataa gaaatactca ataggcttag ctatcggcac aaatagcgtc ggatgggcgg 1921 tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga aatacagacc 1981 gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga gagacagcgg 2041 aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag aatcgtattt 2101 gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt ttctttcatc 2161 gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat cctatttttg 2221 gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat catctgcgaa 2281 aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg gccttagcgc 2341 atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct gataatagtg 2401 atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt gaagaaaacc 2461 ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg agtaaatcaa 2521 gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc ttatttggga 2581 atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt gatttggcag 2641 aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat aatttattgg 2701 cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta tcagatgcta 2761 ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc ctatcagctt 2821 caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa gctttagttc 2881 gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa aacggatatg 2941 caggttatat tgatggggga gctagccaag aagaatttta taaatttatc aaaccaattt 3001 tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa gatttgctgc 3061 gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg ggtgagctgc 3121 atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat cgtgagaaga 3181 ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg cgtggcaata 3241 gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg aattttgaag 3301 aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca aactttgata 3361 aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag tattttacgg 3421 tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa ccagcatttc 3481 tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat cgaaaagtaa 3541 ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat agtgttgaaa 3601 tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat ttgctaaaaa 3661 ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta gaggatattg 3721 ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt aaaacatatg 3781 ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat actggttggg 3841 gacgtttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc aaaacaatat 3901 tagatttttt gaaatcagat ggttttgcca atcgcaattt tatgcagctg atccatgatg 3961 atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa ggcgatagtt 4021 tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt attttacaga 4081 ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca gaaaatatcg 4141 ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat tcgcgagagc 4201 gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag tcagattctt aaagagcatc 4261 ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc caaaatggaa 4321 gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat gatgtcgatg 4381 ccattgttcc acaaagtttc cttaaagacg attcaataga caataaggtc ttaacgcgtt 4441 ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga agaagtagtc aaaaagatga 4501 aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag tttgataatt 4561 taacgaaagc tgaacgtgga ggtttgagtg aacttgataa agctggtttt atcaaacgcc 4621 aattggttga aactcgccaa atcactaagc atgtggcaca aattttggat agtcgcatga 4681 atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt accttaaaat 4741 ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt gagattaaca 4801 attaccatca tgcccatgat gcgtatctaa atgccgtcgt tggaactgct ttgattaaga 4861 aatatccaaa acttgaatcg gagtttgtct atggtgatta taaagtttat gatgttcgta 4921 aaatgattgc taagtctgag caagaaatag gcaaagcaac cgcaaaatat ttcttttact 4981 ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag attcgcaaac 5041 gccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa gggcgagatt 5101 ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag 5161 tccagacagg cggattctcc aaggagtcaa ttttaccaaa aagaaattcg gacaagctta 5221 ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg ttttgatagt ccaacggtag 5281 cttattcagt cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag ttaaaatccg 5341 ttaaagagtt actagggatc acaattctgg aaagaagttc ctttgaaaaa aatccgattg 5401 actttttaga agctaaagga tataaggaag ttaaaaaaga cttaatcatt aaactaccta 5461 aatatagtct ttttgagtta gaaaacggtc gtaaacggat gctggctagt gccggagaat 5521 tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt gaatttttta tatttagcta 5581 gtcattatga aaagttgaag ggtagtccag aagataacga acaaaaacaa ttgtttgtgg 5641 agcagcataa gcattattta gatgagatta ttgagcaaat cagtgaattt tctaagcgtg 5701 ttattttagc agatgccaat ttagataaag ttcttagtgc atataacaaa catagagaca 5761 aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg aatcttggag 5821 ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat acgtctacaa 5881 aagaagtttt agatgccact cttatccatc aatccatcac tggtctttat gaaacacgca 5941 ttgatttgag tcagctagga ggtgactaat aaaagcttga tcagatctca gctttttttt 6001 gaagtgcctg gcagtaagtt gaaaaaattt gaaataaact tggactatgt taataaaaaa 6061 atacaaatta acgaacaaaa agatttgaat tattatataa tttagttaaa agcaatttta 6121 aatgagggag gtttccatat gagggaagcg gtgatcgccg aagtatcgac tcaactatca 6181 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac 6241 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg 6301 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg 6361 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac 6421 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc 6481 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg 6541 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt 6601 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac 6661 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg 6721 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag 6781 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa 6841 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa 6901 ggcgagatca ccaaggtagt cggcaaataa tgacgtcgcc ggagcggtcg agttctggac 6961 cgaccggctc gggttctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 7021 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 7081 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 7141 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 7201 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 7261 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 7321 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 7381 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 7441 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 7501 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 7561 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 7621 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 7681 tgctggcctt ttgctcacat gttctttcct gcactagatc cccggcaagt tcatccttag 7741 ggttttcata tctcttaaaa agtcaatttc tgcttgatta atttcttgtg gattattgtt 7801 tttgcggttt ttcatttttt atctccttga gtattgaatt ttttcttata tataaatata 7861 tagcaaaaac tatttttgcc aactttttta caaaaaattt tttattaaaa cacttagggc 7921 tttgctaaat tctcttttaa aagaacttag taaagcccta tggatttaag aactgattat 7981 acttacgtag taaaaatact acagatatta atagtataga ccatatcaaa atcataatca 8041 atttatattt atctaaaatt gtagtccata caaaatacaa aaaaattagg tttttctaaa 8101 ttctatttta aaagaactta gtaacaccta atctctaaat tacttacaaa attctaaaat 8161 tagattttta gttcttcata tttctttaaa agttttttaa gaaaatcttt ttgattttca 8221 aaaatttcat gcatcataaa actagtaaat ttggattttt ttttgtaaaa atcataactt 8281 tcttgggttt taagttgaaa tcttaatggt tttattggat tttgcctaga tttttttaac 8341 gccggacttt ctttatcttt tataaaatta taagaatttt taataccatt ttctattaaa 8401 taatcttctt ttataagccc ctcttgcagt gcagttgcta atcttaaata cttatacgct 8461 tgagtttttg ctacatcaaa gtcttttata aaatcaagaa aagttttata accatcaaat 8521 ttataatatt gattatcttt tatttctttt aaaattttaa tattttccac cctgtggaat 8581 atttcttttt tgaaattgtc atttaacttt tcctttagtg tcttgtaata agaaatttta 8641 ctttcattaa tcattaaatt ttgttcaaca taagtatcta tcttttttgt taaaacttta 8701 acaacacgtt ttttttccat aaaaattttc ctttattttt gcaacatttt ccttaattga 8761 tataattgtt actattttag attttttaat atccacttaa gaattaatcc ctcactagaa 8821 tattttttat tatagaattc atatacatgt ttttctaaat ctataaattt atcaacaatt 8881 gctttattat agggtgtatc tatcttttct tttcttaata atcgtctcat attctttaaa 8941 taacaacaaa cactgccggt tttaaaccta aattctatgt aatatatttt tgaaaaagta 9001 tatacatttt caaccccgtt tatttcatac tttacaaaaa cattatttat tggttttcta 9061 taaccatagt aaatgcctaa aaatttgtcc ccgtctttca taggatatag ataaagtttt 9121 tgaatctctg atttatctaa taatttttca aaaaaaatta tacaccgctt ttgtttgtta 9181 ctaactactc tatgcaatgc ctttaatatt tttgtgtgat atatattttt atcacctttt 9241 ttttctttca gcaggaagat actttctttt ggaaatttaa ttttttgttc atttattctt 9301 gcagccttaa gtccgatttt catatttaat ccttatatag cctttctatt taattctttt 9361 aaactcaata aacttttttg gtttttcatt agttctaata attcgcaata atatgtgcaa 9421 aacactttat tgtattctat tttcttttgg ttattcaaat attcttttat aattggcttt 9481 agaagttcaa tatttacttc tttttttagt tgttcaataa ggatattgaa aatgtttgtt 9541 tttaaatctt gataattttg ttgagaattt tctttttttc tttcaattga cttctctaat 9601 ttacgcttta tattgtttaa atcgctatat ttatgacttt caataataaa atgaggctta 9661 aatttgtaat tttcgtatat tttttggaaa cttgtttcta attgtttaga attatattca 9721 tttttttcaa attctttttg agtgttgcat agaatttcct ttaatttttc ttgtttttct 9781 ttcatacaag atttagtaaa atgtatattt ttatttttta gtaatttaat ttctattatt 9841 tttatgattt taagtatttt gattatatta tctttatcaa tatttaaatt gaataaaatt 9901 gtaagaaaaa ttgaaagaat ttttttacat aaaaagttac atttattgaa gtaatttctt 9961 acttgatact tttctatttc attaatcttt ctttcttctt ttatattatt tttattattt 10021 aaacactcct ttaaatctac actcccattt ttagtaaatc tatctttaag gccccttgaa 10081 actctatttt gaaatcttaa atcttttttt tcttgaaagt atttatagat tttttggtaa 10141 cattcttttt ttgaaaaatt aagttgatag tatatttcgg tccccaaatt tacccctaag 10201 tgtttgtgat aattagttgt gactttaaat tctttttcta atttgtaaag gtaattttgc 10261 agtgttttta gtttaacggg tgtttgacta tttcttttaa ggttttcatt aaagtaatag 10321 agtatgtttt tttgtgtgta tttcttatat gttttattta tgtacgctag tgttgagata 10381 aggactacta atttatactg taatttattg tagcaagttg tggttttact ggttttgttt 10441 atattagata agttttccat ttatagctcc ttgaataccg gattttttat ctataaagta 10501 tatatatcac aattttgatt taaaagtaaa ttcttttttt aaaaatacaa aaatttttta 10561 taaattatat agaccatgat attgtattaa tactaattga ttttaattta aggttgaact 10621 aaaataaaca tagttttgta gaaaataatt tttcattatt ttgctacttg atattggatc 10681 gtaaaaatat t //