LOCUS Exported 11560 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning. ACCESSION . VERSION . KEYWORDS pBbdCas9H(RBSmut)_arr2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11560) AUTHORS Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C TITLE A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi. JOURNAL Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. PUBMED 33257311 REFERENCE 2 (bases 1 to 11560) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11560 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 160..204 /label=T5 promoter /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 180..196 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 212..228 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 265..4371 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 4558..5577 /codon_start=1 /product="hygromycin B phosphotransferase" /label=HygR /note="confers resistance to hygromycin" /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE" rep_origin 5700..6288 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6189..6208 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgcaaatagc aatagcatta aaaataatta ttattattta tttctttgat taaaatcaaa 61 agcctttaat gtattttctt gatctttcat gcgcttaacg gtaaaatcca aggtattaag 121 ttcaccttcc acttttctct ctctatcttc tctagaaaat cataaaaaat ttatttgctt 181 tgtgagcgga taacaattat aatagattca attgtgagcg gataacaatt tcacacagaa 241 ttcattaaag aagagaaatt acatatggat aagaaatact caataggctt agctatcggc 301 acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc 361 aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta 421 tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat 481 acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa 541 gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag 601 catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat 661 ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc 721 ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga 781 gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac 841 aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt 901 tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag 961 aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt 1021 aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat 1081 gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca 1141 gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata 1201 actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg 1261 actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt 1321 gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt 1381 tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa 1441 ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat 1501 caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt 1561 ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt 1621 ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca 1681 attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt 1741 gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt 1801 ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa 1861 ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc 1921 ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata 1981 gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt 2041 acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat 2101 gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt 2161 gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa 2221 cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat 2281 aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat 2341 tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa 2401 gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct 2461 attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg 2521 cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag 2581 ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga 2641 agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat 2701 ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt 2761 ttaagtgatt atgatgtcga tgccattgtt ccacaaagtt tccttaaaga cgattcaata 2821 gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt 2881 gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc 2941 actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat 3001 aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca 3061 caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag 3121 gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc 3181 tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc 3241 gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat 3301 tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca 3361 accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt 3421 gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt 3481 gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc 3541 aatattgtca agaaaacaga agtccagaca ggcggattct ccaaggagtc aattttacca 3601 aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt 3661 ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg 3721 aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattct ggaaagaagt 3781 tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa 3841 gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg 3901 atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat 3961 gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac 4021 gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa 4081 atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt 4141 gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta 4201 tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat 4261 cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc 4321 actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgacta ataaaagctt 4381 gatcagatcc cagctttttt tgaagtgcct ggcagtaagt tgaaaaaatt tgaaataaac 4441 ttggactatg ttaataaaaa aatacaaatt aacgaacaaa aagatttgaa ttattatata 4501 atttagttaa aagcaatttt aaatgaggga ggtttccatc atatggatag aagtggtaaa 4561 cctgaattaa ctgctactag tgttgaaaaa tttttaattg aaaaatttga tagtgttagt 4621 gatttaatgc aattaagtga aggtgaagaa agtagagctt ttagttttga tgttggtggt 4681 agaggttatg ttttaagagt taatagttgt gctgatggtt tttataaaga tagatatgtt 4741 tatagacatt ttgctagtgc tgctttacct attcctgaag ttttagatat tggtgaattt 4801 agtgaaagtt taacttattg tattagtaga agagcacaag gtgttacttt acaagattta 4861 cctgaaactg aattacctgc tgttttacaa cctgttgctg aagctatgga tgctattgcg 4921 gcggctgatc tcagtcaaac ttcgggtttt ggtccttttg gtcctcaagg tattggtcaa 4981 tatactactt ggagagattt tatttgtgct attgctgatc ctcatgttta tcattggcaa 5041 actgttatgg atgatactgt tagtgctagt gttgctcaag cattagatga attaatgtta 5101 tgggctgaag attgtcctga agttagacat ttagttcatg ctgattttgg tagtaataat 5161 gttttaactg ataatggtag aattactgct gttattgatt ggagtgaagc tatgtttggt 5221 gatagtcaat atgaagttgc taatattttt ttttggagac cttggttagc ttgtatggaa 5281 caacaaacta gatattttga aagaagacat cctgaattag ctggtagtcc tagattaaga 5341 gcttatatgt taagaattgg tttagatcaa ttatatcaaa gtttagttga tggtaatttt 5401 gatgatgctg cttgggctca aggtagatgt gatgctattg ttagaagtgg tgctggtact 5461 gttggtagaa ctcaaattgc tagaagaagt gctgctgttt ggactgatgg ttgtgttgaa 5521 gttttagctg atagtggtaa tagaagacct agtactagac ctagagctaa agaataatga 5581 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcatgac caaaatccct 5641 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 5701 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 5761 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 5821 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 5881 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 5941 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 6001 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 6061 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 6121 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 6181 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 6241 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 6301 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgca 6361 ctagatcccc ggcaagttca tccttagggt tttcatatct cttaaaaagt caatttctgc 6421 ttgattaatt tcttgtggat tattgttttt gcggtttttc attttttatc tccttgagta 6481 ttgaattttt tcttatatat aaatatatag caaaaactat ttttgccaac ttttttacaa 6541 aaaatttttt attaaaacac ttagggcttt gctaaattct cttttaaaag aacttagtaa 6601 agccctatgg atttaagaac tgattatact tacgtagtaa aaatactaca gatattaata 6661 gtatagacca tatcaaaatc ataatcaatt tatatttatc taaaattgta gtccatacaa 6721 aatacaaaaa aattaggttt ttctaaattc tattttaaaa gaacttagta acacctaatc 6781 tctaaattac ttacaaaatt ctaaaattag atttttagtt cttcatattt ctttaaaagt 6841 tttttaagaa aatctttttg attttcaaaa atttcatgca tcataaaact agtaaatttg 6901 gatttttttt tgtaaaaatc ataactttct tgggttttaa gttgaaatct taatggtttt 6961 attggatttt gcctagattt ttttaacgcc ggactttctt tatcttttat aaaattataa 7021 gaatttttaa taccattttc tattaaataa tcttctttta taagcccctc ttgcagtgca 7081 gttgctaatc ttaaatactt atacgcttga gtttttgcta catcaaagtc ttttataaaa 7141 tcaagaaaag ttttataacc atcaaattta taatattgat tatcttttat ttcttttaaa 7201 attttaatat tttccaccct gtggaatatt tcttttttga aattgtcatt taacttttcc 7261 tttagtgtct tgtaataaga aattttactt tcattaatca ttaaattttg ttcaacataa 7321 gtatctatct tttttgttaa aactttaaca acacgttttt tttccataaa aattttcctt 7381 tatttttgca acattttcct taattgatat aattgttact attttagatt ttttaatatc 7441 cacttaagaa ttaatccctc actagaatat tttttattat agaattcata tacatgtttt 7501 tctaaatcta taaatttatc aacaattgct ttattatagg gtgtatctat cttttctttt 7561 cttaataatc gtctcatatt ctttaaataa caacaaacac tgccggtttt aaacctaaat 7621 tctatgtaat atatttttga aaaagtatat acattttcaa ccccgtttat ttcatacttt 7681 acaaaaacat tatttattgg ttttctataa ccatagtaaa tgcctaaaaa tttgtccccg 7741 tctttcatag gatatagata aagtttttga atctctgatt tatctaataa tttttcaaaa 7801 aaaattatac accgcttttg tttgttacta actactctat gcaatgcctt taatattttt 7861 gtgtgatata tatttttatc accttttttt tctttcagca ggaagatact ttcttttgga 7921 aatttaattt tttgttcatt tattcttgca gccttaagtc cgattttcat atttaatcct 7981 tatatagcct ttctatttaa ttcttttaaa ctcaataaac ttttttggtt tttcattagt 8041 tctaataatt cgcaataata tgtgcaaaac actttattgt attctatttt cttttggtta 8101 ttcaaatatt cttttataat tggctttaga agttcaatat ttacttcttt ttttagttgt 8161 tcaataagga tattgaaaat gtttgttttt aaatcttgat aattttgttg agaattttct 8221 ttttttcttt caattgactt ctctaattta cgctttatat tgtttaaatc gctatattta 8281 tgactttcaa taataaaatg aggcttaaat ttgtaatttt cgtatatttt ttggaaactt 8341 gtttctaatt gtttagaatt atattcattt ttttcaaatt ctttttgagt gttgcataga 8401 atttccttta atttttcttg tttttctttc atacaagatt tagtaaaatg tatattttta 8461 ttttttagta atttaatttc tattattttt atgattttaa gtattttgat tatattatct 8521 ttatcaatat ttaaattgaa taaaattgta agaaaaattg aaagaatttt tttacataaa 8581 aagttacatt tattgaagta atttcttact tgatactttt ctatttcatt aatctttctt 8641 tcttctttta tattattttt attatttaaa cactccttta aatctacact cccattttta 8701 gtaaatctat ctttaaggcc ccttgaaact ctattttgaa atcttaaatc ttttttttct 8761 tgaaagtatt tatagatttt ttggtaacat tctttttttg aaaaattaag ttgatagtat 8821 atttcggtcc ccaaatttac ccctaagtgt ttgtgataat tagttgtgac tttaaattct 8881 ttttctaatt tgtaaaggta attttgcagt gtttttagtt taacgggtgt ttgactattt 8941 cttttaaggt tttcattaaa gtaatagagt atgttttttt gtgtgtattt cttatatgtt 9001 ttatttatgt acgctagtgt tgagataagg actactaatt tatactgtaa tttattgtag 9061 caagttgtgg ttttactggt tttgtttata ttagataagt tttccattta tagctccttg 9121 aataccggat tttttatcta taaagtatat atatcacaat tttgatttaa aagtaaattc 9181 tttttttaaa aatacaaaaa ttttttataa attatataga ccatgatatt gtattaatac 9241 taattgattt taatttaagg ttgaactaaa ataaacatag ttttgtagaa aataattttt 9301 cattattttg ctacttgata ttggatcgta aaaatattag ggctttacaa agttctttta 9361 aaagagaatt tagtaaagcc ctaagtcttt taataaaaaa ttttttgtaa aaaagttggc 9421 aaaaatagtt tttgctatat atttatatat aagaaaaaat tcaatactca aggagataaa 9481 aaatggaaaa cttaaacaat aatccacaaa aaattaatca aataaataag caaactaatt 9541 ttattggttt tgaaaatttt atagaacttg ataatcaagt taatcaaact aaagaaaaaa 9601 gtaaattaag taagataggt aagaaattac ccggaataag cagtcaagtc tagtgcggcc 9661 gcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc 9721 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc 9781 agggcagtcg ccctaaaaca aagttaaaca tcatgaggag gtaacgtcat ggtaaaagat 9841 tggattccta tttctcatga taattataaa caagtacaag gaccatttta tcatggaact 9901 aaagctaatt tagcaattgg tgatttacta actacaggat ttatttctca ttttgaagat 9961 ggtcgaattc ttaaacatat ttatttttca gctttaatgg aaccagcagt ttggggagct 10021 gaacttgcta tgtcactatc tggtcttgaa ggtcgtggtt atatatatat agttgaacca 10081 acaggaccat ttgaagatga tccaaatctt acaaataaaa aatttcctgg taatccaaca 10141 caatcttata gaacttgtga acctttaaga attgttggtg ttgttgaaga ttgggaagga 10201 catcctgttg aattaataag aggaatgtta gattcattag aagatttaaa acgtcgtggt 10261 ttacatgtta ttgaagatta acctggcgcg ccaaataatg cccgtgtccc tgttgaatag 10321 ggacacggtt ttttaacaat ttttttattc ctaggcctta gtggccggta cccggggatc 10381 cttattactg gccgctttct agcctggata cttgtctggc tagttgcatt agagagtcag 10441 ctagagccct agggctagct gtttgtgtat tcggagccag tgtagttttt ctttttacta 10501 acgatacggg taataattga ttgcctttta cagcctgacc ttgagacaat tgcaaaagtc 10561 tgtccacaga ggtttgacct aataatctaa aatcttgttt gatagtagta agtggaggta 10621 tgtagcaact agaatcttct gtatcatcat aacccacaac tgaaatatca gccccaactc 10681 taagtccact ttcagtaata gctcgcatag ctcctaaagc catttgatca ttagcaacta 10741 acatagcagt agggacaatg ccttcattta acatttgcat tgtttgctga aatcctgaca 10801 tagcagacca atctccttct ctttctgcta ttggttgtat ttgatttctt gttaaatact 10861 tgtgccatcc tgctaatctc aatctggcac ttactgaact taagggacct gcaagtagtg 10921 caatttgttg atggcctaat gcaacaaggt gttcaactcc taaccttgtt ccatcttcgt 10981 gagaaaaaat tatagagttt attggggttt ggtccgacac atccaaaaac aatgctggaa 11041 cattagtaca tgctgcttca actgcaatcg catcttggtc atcaagagga taattaatta 11101 taagtccact aacacgttga gctaataaat tatgtactgc tgccttacaa gcctctacac 11161 ctgatctctc aaccattgat acaacaacgc ttgcaccaag ttgatcagct ctcgatttaa 11221 ttgcggctac aatttgacta ggcgcatgta atgcaagtga tgatgtggca actccaatta 11281 aaagtgattg tttccccgca agttgctgag caacgcgatt tggtatataa ttcaactctg 11341 ccatagctgc ttcaaccttc tctcttgttt ttgcagatac atgacttgct tggttcacaa 11401 ctctcgaaac cgtttggtag ctaaccccgg catattcagc aacatcatac aaagtaacag 11461 gtttcatgga tcattcctcc atgataaaat ttaaatttct gactttggca aatccttttg 11521 cctgttaaaa agaatagcac aaaaattaaa aaaaaagaaa //