LOCUS       Exported                9232 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    pDONR207 SARS-CoV2 TEV-NSP3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    17..116
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             116..136
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(5979..6078)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6190..6209)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(6268..6287)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(6861..7394)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(7583..7611)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      8268..8856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8757..8776
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      8991..9018
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      9110..9196
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 catggatctc gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca
       61 acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag ttggcgaaaa
      121 cctgtacttc caaagtatgg cccctaccaa ggtgaccttc ggagacgaca ccgtgatcga
      181 ggtgcagggt tacaagagcg tgaacatcac cttcgagctg gacgaacgca tcgacaaggt
      241 gctgaacgaa aagtgctccg cttacaccgt ggagctgggc accgaggtga acgaattcgc
      301 ctgcgtggtg gccgacgctg tgatcaagac cctgcagcca gtgtccgaac tgctgacccc
      361 tctgggtatc gacctggacg agtggtctat ggctacctac tacctgttcg acgagagcgg
      421 cgaattcaag ctggcctctc acatgtactg cagcttctac cctcccgacg aagacgagga
      481 agagggtgac tgcgaagagg aagagttcga gccttccact cagtacgagt acggcaccga
      541 agacgactac cagggcaagc ctctggaatt cggagccacc tctgccgctc tgcagcctga
      601 agaggaacag gaggaagact ggctggacga cgactcccag cagaccgtgg gtcagcagga
      661 cggctctgaa gacaaccaga ccaccaccat ccagaccatc gtggaggtgc agcctcagct
      721 ggagatggaa ctgaccccag tggtgcagac catcgaggtg aacagcttct ccggctacct
      781 gaagctgacc gacaacgtgt acatcaagaa cgctgacatc gtggaggaag ccaagaaggt
      841 gaaaccaacc gtggtggtga acgccgctaa cgtgtacctg aagcacggcg gaggtgtggc
      901 tggagctctg aacaaggcta ccaacaacgc catgcaggtg gaatccgacg actacatcgc
      961 taccaacggt cctctgaagg tgggcggatc ctgcgtgctg tctggccaca acctggccaa
     1021 gcactgcctg cacgtggtgg gaccaaacgt gaacaaggga gaggacatcc agctgctgaa
     1081 gagcgcctac gaaaacttca accagcacga ggtgctgctg gctccactgc tgtctgccgg
     1141 aatcttcggt gctgatccca tccacagcct gcgcgtgtgc gtggacaccg tgaggaccaa
     1201 cgtgtacctg gccgtgttcg acaagaacct gtacgacaag ctggtgagct ccttcctgga
     1261 gatgaagtcc gaaaagcagg tggagcagaa gatcgccgag atccccaagg aggaagtgaa
     1321 gcctttcatc accgaatcta agcccagcgt ggagcagaga aagcaggacg acaagaagat
     1381 caaggcctgc gtggaggaag tgaccaccac cctggaggaa accaagttcc tgaccgagaa
     1441 cctgctgctg tacatcgaca tcaacggcaa cctgcaccct gactccgcta ccctggtgtc
     1501 tgacatcgac atcaccttcc tgaagaagga cgccccctac atcgtgggcg acgtggtgca
     1561 ggaaggagtg ctgaccgctg tggtcatccc taccaagaag gccggtggca ccaccgagat
     1621 gctggccaag gctctgcgta aggtgccaac cgacaactac atcaccacct accccggtca
     1681 gggcctgaac ggttacaccg tggaggaagc taagaccgtg ctgaagaagt gcaagtctgc
     1741 cttctacatc ctgccctcca tcatctctaa cgagaagcag gaaatcctgg gcaccgtgag
     1801 ctggaacctg cgcgaaatgc tggcccacgc tgaggaaacc aggaagctga tgcccgtgtg
     1861 cgtggagacc aaggccatcg tgagcaccat ccagagaaag tacaagggca tcaagatcca
     1921 ggagggtgtg gtggactacg gcgctcgttt ctacttctac acctccaaga ccaccgtggc
     1981 ctctctgatc aacaccctga acgacctgaa cgagaccctg gtgaccatgc cactgggata
     2041 cgtgacccac ggtctgaacc tggaggaagc cgctagatac atgcgttccc tgaaggtgcc
     2101 tgctaccgtg agcgtgagca gccctgacgc tgtgaccgcc tacaacggat acctgacctc
     2161 ctctagcaag acccctgagg aacacttcat cgaaaccatc agcctggccg gttcctacaa
     2221 ggactggtct tacagcggac agtccaccca gctgggcatc gagttcctga agcgcggaga
     2281 caagagcgtg tactacacct ccaacccaac caccttccac ctggacggtg aagtgatcac
     2341 cttcgacaac ctgaagaccc tgctgtctct gcgcgaggtg aggaccatca aggtgttcac
     2401 caccgtggac aacatcaacc tgcacaccca ggtggtggac atgagcatga cctacggaca
     2461 gcagttcggt cctacctacc tggacggagc tgacgtgacc aagatcaagc cacacaactc
     2521 ccacgagggc aagaccttct acgtgctgcc taacgatgac accctgagag tggaggcttt
     2581 cgaatactac cacaccaccg acccatcctt cctgggcagg tacatgtctg ccctgaacca
     2641 caccaagaag tggaagtacc cccaggtgaa cggactgacc agcatcaagt gggccgacaa
     2701 caactgctac ctggccaccg ctctgctgac cctgcagcag atcgaactga agttcaaccc
     2761 acccgctctg caggacgctt actacagagc ccgtgctggc gaggccgcta acttctgcgc
     2821 tctgatcctg gcctactgca acaagaccgt gggcgaactg ggagacgtga gagagaccat
     2881 gagctacctg ttccagcacg ccaacctgga ctcctgcaag cgtgtgctga acgtggtgtg
     2941 caagacctgc ggccagcagc agaccaccct gaagggagtg gaagctgtga tgtacatggg
     3001 taccctgtcc tacgagcagt tcaagaaggg cgtgcagatc ccttgcacct gcggaaagca
     3061 ggccaccaag tacctggtgc agcaggaaag cccattcgtg atgatgtccg ctcctccagc
     3121 ccagtacgaa ctgaagcacg gcaccttcac ctgcgcttcc gagtacaccg gcaactacca
     3181 gtgcggacac tacaagcaca tcacctctaa ggagaccctg tactgcatcg acggagccct
     3241 gctgaccaag tcctctgaat acaagggtcc catcaccgac gtgttctaca aggagaacag
     3301 ctacaccacc accatcaagc ccgtgaccta caagctggac ggcgtggtgt gcaccgaaat
     3361 cgaccctaag ctggacaact actacaagaa ggacaactct tacttcaccg agcagcctat
     3421 cgacctggtg ccaaaccagc cctaccctaa cgccagcttc gacaacttca agttcgtgtg
     3481 cgacaacatc aagttcgctg acgacctgaa ccagctgacc ggatacaaga agcctgcctc
     3541 tagagagctg aaggtgacct tcttcccaga cctgaacggc gacgtggtgg ctatcgacta
     3601 caagcactac accccaagct tcaagaaggg agccaagctg ctgcacaagc ccatcgtgtg
     3661 gcacgtgaac aacgctacca acaaggccac ctacaagcct aacacctggt gcatccgttg
     3721 cctgtggtct accaagccag tggaaacctc caactctttc gacgtgctga agagcgagga
     3781 cgctcagggc atggacaacc tggcctgcga agacctgaag cccgtgagcg aggaagtggt
     3841 ggagaaccca accatccaga aggacgtgct ggaatgcaac gtgaagacca ccgaggtggt
     3901 gggagacatc atcctgaagc cagctaacaa cagcctgaag atcaccgagg aagtgggtca
     3961 caccgacctg atggccgctt acgtggacaa cagctccctg accatcaaga agcccaacga
     4021 gctgagcagg gtgctgggtc tgaagaccct ggctactcac ggtctggctg ctgtgaactc
     4081 tgtgccatgg gacaccatcg ctaactacgc caagcctttc ctgaacaagg tggtgtccac
     4141 caccaccaac atcgtgacca gatgtctgaa ccgcgtgtgc accaactaca tgccctactt
     4201 cttcaccctg ctgctgcagc tgtgcacctt caccagaagc accaactccc gtatcaaggc
     4261 tagcatgcct accaccatcg ccaagaacac cgtgaagagc gtgggaaagt tctgcctgga
     4321 ggcttccttc aactacctga agtctccaaa cttcagcaag ctgatcaaca tcatcatctg
     4381 gttcctgctg ctctccgtgt gcctgggttc cctgatctac tctaccgccg ctctgggagt
     4441 gctgatgtct aacctgggta tgcctagcta ctgcactggt tacagagagg gatacctgaa
     4501 cagcaccaac gtgaccatcg ccacctactg caccggcagc atcccatgct ccgtgtgcct
     4561 gtctggactg gacagcctgg acacctaccc ctccctggaa accatccaga tcaccatctc
     4621 tagcttcaag tgggacctga ccgccttcgg tctggtggct gagtggttcc tggcctacat
     4681 cctgttcacc aggttcttct acgtgctggg cctggccgct atcatgcagc tgttcttctc
     4741 ctacttcgct gtgcacttca tcagcaactc ctggctgatg tggctgatca tcaacctggt
     4801 gcagatggct cccatctctg ccatggtgcg catgtacatc ttcttcgcca gcttctacta
     4861 cgtgtggaag tcctacgtgc acgtggtgga cggttgcaac tcctctacct gcatgatgtg
     4921 ctacaagcgc aacagggcta ccagggtgga gtgcaccacc atcgtgaacg gcgtgcgcag
     4981 gtccttctac gtgtacgcca acggcggcaa gggattctgc aagctgcaca actggaactg
     5041 cgtgaactgc gacaccttct gcgctggctc caccttcatc tctgacgagg tggccagaga
     5101 cctgagcctg cagttcaagc gtccaatcaa ccccaccgac cagagctcct acatcgtgga
     5161 ctctgtgacc gtgaagaacg gtagcatcca cctgtacttc gacaaggctg gccagaagac
     5221 ctacgagcgc cactctctga gccacttcgt gaacctggac aacctgaggg ccaacaacac
     5281 caagggcagc ctgcctatca acgtgatcgt gttcgacgga aagtccaagt gcgaggaatc
     5341 tagcgccaag tccgccagcg tgtactacag ccagctgatg tgccagccaa tcctgctgct
     5401 ggaccaggct ctggtgtctg acgtgggaga cagcgccgaa gtggccgtga agatgttcga
     5461 cgcctacgtg aacaccttct cctctacctt caacgtgcct atggagaagc tgaagaccct
     5521 ggtggctact gctgaggctg aactggctaa gaacgtgtcc ctggacaacg tgctgagcac
     5581 cttcatctcc gccgctaggc agggtttcgt ggactctgac gtggaaacca aagacgtggt
     5641 ggagtgcctg aagctgtctc accagagcga catcgaagtg accggcgact cctgcaacaa
     5701 ctacatgctg acctacaaca aggtggagaa catgacccca agagacctgg gagcttgcat
     5761 cgactgctcc gcccgtcaca tcaacgctca ggtggccaag tctcacaaca tcgccctgat
     5821 ctggaacgtg aaggacttca tgagcctgtc cgagcagctg cgcaagcaga tcaggagcgc
     5881 cgctaagaag aacaacctgc ccttcaagct gacctgcgct accaccagac aggtggtgaa
     5941 cgtggtgacc accaagatcg ccctgaaggg cggataatac ccaacttttt tgtacaaagt
     6001 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     6061 aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa aatctctgat
     6121 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     6181 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     6241 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     6301 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     6361 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     6421 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     6481 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     6541 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     6601 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     6661 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     6721 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     6781 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaggc cgggaagccg
     6841 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     6901 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     6961 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
     7021 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     7081 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     7141 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     7201 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     7261 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     7321 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     7381 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     7441 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
     7501 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     7561 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     7621 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga
     7681 ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc
     7741 attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc cctggcttca
     7801 ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt
     7861 ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg actctagcta
     7921 tagttctagt ggttggctac taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     7981 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     8041 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     8101 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     8161 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagttt
     8221 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8281 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8341 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8401 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8461 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8521 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8581 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8641 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8701 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8761 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8821 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8881 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8941 tctgtggata accgtattac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     9001 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     9061 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     9121 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     9181 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct ag
//
