LOCUS Exported 10240 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS DSCAM_pCSdest SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10240) AUTHORS Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, McCallion AS, Katsanis N, Reeves RH TITLE Survey of Human Chromosome 21 Gene Expression Effects on Early Development in Danio rerio. JOURNAL G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 10.1534/g3.118.200144. PUBMED 29760202 REFERENCE 2 (bases 1 to 10240) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10240 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..978 /label=CMV IE94 promoter /note="enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94" promoter 1013..1031 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 1013..1030 /label=SP6 /note="SP6 promoter, forward primer" protein_bind 1085..1109 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(7202..7226) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter complement(7248..7265) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 7270..7404 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7294..7313) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 7348..7367 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(7517..7537) /label=T3 /note="T3 promoter, forward primer" promoter complement(7517..7535) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7556..7572) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7556..7572) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7569..7591) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7580..7596 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7604..7634) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7649..7670 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7787..7804) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7958..8546) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8038..8057) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8717..9577) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9340..9359 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9578..9682) /gene="bla" /label=AmpR promoter rep_origin complement(9708..10163) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9845..9866) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 10057..10076 /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt 61 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca 121 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ctggcactgt 181 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg 241 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat 301 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca 361 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt 421 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac 481 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac 541 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta 601 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa 661 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg 721 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac 781 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg 841 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa 901 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc 961 aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt 1021 gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt cgaattcaag 1081 gatcacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca tgtggatact 1141 ggctctctcc ttgttccaga gcttcgcgaa tgttttcagt gaagacctac actccagcct 1201 ctactttgtc aatgcatctc tgcaagaggt agtgtttgcc agcaccacgg ggactctggt 1261 gccctgcccc gcagcaggca tccctcctgt gactctcaga tggtacctag ccacgggcga 1321 ggagatctac gatgtccccg ggatccgcca cgtccacccc aacggcactc tccaaatttt 1381 ccccttccct ccttcaagct tcagtacctt aatccatgat aatacttatt attgcacagc 1441 tgaaaatcct tcagggaaaa ttagaagtca ggatgtccac atcaaggctg ttttacggga 1501 gccctataca gtccgtgtgg aggaccagaa aaccatgaga ggcaatgttg cggtcttcaa 1561 gtgcattatc ccctcctcgg tggaggcgta catcactgtc gtctcatggg agaaagacac 1621 tgtttcactt gtctcaggat ctagatttct catcacatcc acgggagcct tgtatattaa 1681 agatgtacag aatgaagatg gattgtataa ctaccgctgc atcacgcggc atcgatacac 1741 cggagagacg aggcagagca acagcgccag actttttgta tcagacccag cgaactcagc 1801 cccatccata ctggatgggt ttgagcatcg caaagccatg gctgggcagc gtgtggagct 1861 gccttgcaaa gcgctcgggc accctgagcc agattaccgc tggctgaagg acaacatgcc 1921 cctggaactt tcagggaggt tccagaagac cgtgacgggg ctgctcattg agaacattcg 1981 cccctcggac tcaggcagct atgtttgtga agtgtccaac agatacggaa ctgctaaggt 2041 gataggccgc ctgtacgtga aacagccact gaaagccacc atcagtccca ggaaggttaa 2101 aagcagcgtg ggtagccaag tttccttgtc ctgcagcgtg acaggaactg aggaccagga 2161 actctcctgg taccgcaatg gtgaaatcct caaccctgga aaaaatgtga ggatcacagg 2221 gatcaaccac gaaaacctta taatggatca catggtcaaa agtgacgggg gcgcatacca 2281 gtgctttgtg cgcaaggaca agctgtccgc tcaagactat gtgcaggtgg tccttgaaga 2341 tggaactccc aaaattattt ctgcctttag tgaaaaggtg gtgagtccag cagagccggt 2401 ttcccttatg tgcaacgtga agggaacacc tttgcccacg atcacgtgga ccctggacga 2461 tgacccgatt ctcaagggtg gcagtcaccg catcagccag atgatcacgt cggaggggaa 2521 cgtggtcagc tacctgaaca tctccagctc ccaggtccgg gacgggggag tctaccgctg 2581 cactgccaac aactcggcgg gagtcgtcct gtaccaggct cgaataaacg taagagggcc 2641 tgcaagcatt cgaccaatga aaaacatcac agcaatagca ggacgggaca catacattca 2701 ctgtcgtgtg attggctatc cgtattactc cattaaatgg tacaagaact ctaacctgct 2761 tcctttcaac caccgccaag tggcatttga gaacaatgga actcttaaac tttcagatgt 2821 gcaaaaggaa gtggacgagg gggagtacac gtgcaacgtg ttggttcaac cacaactctc 2881 caccagccag agcgtccacg tgaccgtgaa agttccgcct ttcatacaac cctttgagtt 2941 tccaagattc tccattgggc agcgggtctt catcccctgt gttgtggtct caggggactt 3001 acccatcacg atcacctggc agaaggatgg ccggccaatc cctgggagcc ttggggtgac 3061 cattgacaat attgacttca cgagctcctt gaggatttcc aatctctcgc tcatgcacaa 3121 tgggaattac acctgcatag cccggaatga ggccgccgct gtggagcacc aaagccagtt 3181 gattgtcaga gttcctccca agtttgtggt tcagccacgg gaccaggacg ggatttatgg 3241 caaagcagtc atcctcaatt gttctgctga gggttaccct gtacctacca tcgtgtggaa 3301 attctctaaa ggtgctgggg ttccccagtt ccagccaatt gccctaaatg gccgaatcca 3361 agttctcagc aatgggtcgt tgctgatcaa gcatgtcgtg gaggaagaca gtggctacta 3421 cctctgcaag gtcagcaacg atgtgggcgc agacgtcagc aagtccatgt acctcacggt 3481 taaaattcct gcgatgataa catcctatcc aaatactacc ctggccacgc aggggcagaa 3541 aaaggagatg agctgcacgg cgcatggtga gaagcccatt atagtccgct gggagaagga 3601 ggaccgaatc attaaccctg agatggcccg ttatcttgtg tccaccaagg aggtgggaga 3661 agaggtgatt tctactctgc agattttgcc aactgtgaga gaagattctg gtttcttttc 3721 ctgccatgct attaattctt atggggagga ccgtggaata attcagctca cagtgcaaga 3781 gcccccagac cctcccgaaa ttgagatcaa agatgtcaaa gcacgcacaa ttacgctcag 3841 gtggaccatg gggtttgatg gaaacagtcc catcacaggc tacgatattg aatgcaaaaa 3901 taaatcagac tcctgggatt ctgctcagag aaccaaagat gtttcccctc agctgaactc 3961 ggccaccatc attgatatcc acccttcctc cacctacagc atccgcatgt acgccaagaa 4021 ccggattggc aagagcgagc ccagcaacga gctcaccatc acggcggacg aggcagctcc 4081 tgatggtcca cctcaggaag ttcacctgga gcctatatca tctcagagca tcagggtcac 4141 atggaaggct cccaagaaac atttgcaaaa tgggattatc cgtggctacc aaataggtta 4201 ccgagagtac agcactgggg gtaacttcca attcaacatt atcagtgtcg acaccagcgg 4261 ggacagtgag gtttacaccc tggacaacct gaataagttc actcagtacg gcctggtggt 4321 gcaggcctgt aaccgggccg gcacggggcc ttcttctcag gaaatcatca ccaccactct 4381 cgaggatgtg cccagttacc cccccgaaaa tgtccaagcc atagcaacat caccagaaag 4441 catatcaata tcctggtcca cactttccaa ggaagccttg aatggaattc tccaggggtt 4501 cagagtcatt tactgggcca acctcatgga cggagagctg ggtgagatta aaaacatcac 4561 caccacacag ccttcactgg agctggacgg gctggaaaag tacaccaact acagcatcca 4621 ggtgctggcc ttcacccgcg caggagacgg ggtcaggagt gagcagatct tcacccggac 4681 caaagaggat gttccaggtc ctcccgcggg tgtgaaggca gcggcggcct cagcctccat 4741 ggtctttgtg tcctggcttc cccctctcaa gctgaacggc atcatccgaa agtacactgt 4801 attctgctcc cacccctatc ccacagtgat cagcgagttt gaggcctctc ccgactcgtt 4861 ttcctacaga attcccaacc tgagtaggaa tcgtcagtac agcgtctggg tggtggctgt 4921 tacttcagcc ggaagaggca acagcagtga aatcatcaca gttgagccac tagcaaaagc 4981 tcctgcacga atcctgacct tcagtgggac agtgactact ccatggatga aagacattgt 5041 cttgccttgt aaggctgttg gggacccttc tcctgcagtc aaatggatga aagacagtaa 5101 cgggacaccc agtctagtaa cgattgatgg gcggaggagc atctttagca acggaagctt 5161 cattattcgc acggtgaaag cagaagactc cggctattac agctgcattg ccaataacaa 5221 ctggggatct gatgaaatta ttttaaactt acaagtacaa gttccaccag atcagcctcg 5281 gcttacagtc tccaagacca cgtcttcctc catcaccctt tcttggctcc ctggagacaa 5341 cgggggcagc tctatcagag gatacatact gcagtactcc gaggacaata gtgagcagtg 5401 ggggagtttt ccaatcagcc ccagcgaacg ttcctatcgc ttggaaaatc tcaaatgtgg 5461 gacttggtat aagttcacac tgacagccca aaatggagtg ggcccagggc gcataagtga 5521 aatcatagaa gcaaagacct taggaaaaga gccccagttc tcaaaggagc aggagctgtt 5581 tgccagcatc aacaccacac gcgtgaggct gaacctcatt ggctggaatg atggcggctg 5641 ccccatcacc tccttcacac tagagtacag gccctttggg accacagttt ggaccacagc 5701 tcagaggacc tctctctcca agtcctacat cctgtatgac ctgcaggaag ccacctggta 5761 tgagctgcag atgcgggtgt gcaacagtgc gggctgcgcg gagaagcagg ccaacttcgc 5821 tacgctgaac tacgatggca gtacaattcc tccactcatt aagtcagttg tccaaaacga 5881 agaagggctg acgaccaacg aggggctcaa gatgctggtg accatctcct gtatcctggt 5941 gggggtcttg ctgctgtttg tgctcctgct ggttgtgcgg aggaggcggc gggagcagag 6001 gctaaagagg ctgcgagatg caaagagttt agctgaaatg ctcatgagta agaatacccg 6061 gacttcagat acgttaagca agcaacagca gaccctgcga atgcacatcg acatacccag 6121 ggctcagctt ttgattgaag agagagacac gatggagacc attgatgatc gctccacggt 6181 tctgttgacg gatgctgact ttggagaggc agctaagcag aagtccctga cggtcactca 6241 cacggtccat taccaatcgg tgtctcaggc cactgggccc ttagtggatg tttcagacgc 6301 tcggccggga acgaatccca ccaccaggag gaatgccaag gctgggccca cagcgagaaa 6361 ccgctatgcc agccagtgga ccctcaaccg accccacccc accatctcag cacacaccct 6421 caccacagac tggaggctgc caacacccag ggctgcagga tcagtagaca aagagagcga 6481 cagttacagc gtcagcccct cgcaagacac agatcgagca agaagcagca tggtctccac 6541 agaaagtgcc tcctccactt acgaagaact ggccagggcc tacgaacacg ccaagatgga 6601 agagcaactg aggcacgcca agttcaccat cacggagtgc ttcatatcag acacgtcatc 6661 ggagcagttg acggcaggga caaatgagta cacggacagt ctgacctcca gcaccccttc 6721 cgaatcggga atctgcaggt tcactgcatc tccccccaaa cctcaggatg gaggaagagt 6781 aatgaatatg gcagttccaa aggcacatcg gccaggtgac ctcatacatt tgcctccata 6841 ccttagaatg gactttttgt taaaccgagg tggtccaggc accagcaggg acctgagctt 6901 aggacaagca tgcttggaac ctcagaaaag ccggaccctg aagcgcccca cggtcctgga 6961 gcccatcccg atggaagccg cctcctccgc ctcctccacg agagaaggac agtcgtggca 7021 gccgggggcc gtggccacat tacctcagcg ggagggagca gagctgggac aggcagctaa 7081 aatgagcagc tcccaagaat cactgctcga ctcccggggc catttgaaag gaaacaatcc 7141 ttacgcaaaa tcttacaccc tggtagttta aacgaattcg agctcaaggg tgggcgcgcc 7201 gacccagctt tcttgtacaa agtggtgatc ctctcgagcc tctagaacta tagtgagtcg 7261 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga 7321 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc 7381 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt 7441 cagggggagg tgtgggaggt tttttaattc gcggccgcgg cgccaatgca ttgggcccgg 7501 tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat 7561 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggga 7621 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 7681 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 7741 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 7801 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 7861 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 7921 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 7981 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 8041 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 8101 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 8161 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 8221 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 8281 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 8341 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 8401 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 8461 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 8521 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 8581 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 8641 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 8701 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 8761 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 8821 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 8881 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 8941 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 9001 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 9061 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 9121 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 9181 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 9241 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 9301 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 9361 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 9421 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 9481 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 9541 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 9601 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 9661 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt 9721 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata 9781 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 9841 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 9901 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 9961 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 10021 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 10081 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 10141 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg 10201 gcgatcggtg cgggcctctt cgctattacg ccagtcgacc //