LOCUS       Exported               10080 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  A S. pneumoniae not integrated, temperature sensitive plasmid that 
            carries the CRISPR system; a Zn inducible wtCas9 and a sgRNA which 
            has its spacer sequence replaced by gfp gene.
ACCESSION   .
VERSION     .
KEYWORDS    pDS05
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Synefiaridou D, Veening J-W
  TITLE     Harnessing CRISPR-Cas9 for genome editing in Streptococcus 
            pneumoniae
  JOURNAL   bioRxiv https://www.biorxiv.org/content/10.1101/2020.06.13.149682v2
REFERENCE   2  (bases 1 to 10080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      791..1529
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        1577..1668
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1669..2460
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             2713..6819
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLAKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSNNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVRKDLIVKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATFIHQSITGLYETRIDLSQLGGD"
     CDS             7038..7754
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             9376..9387
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gagtgattac atgaacaaaa atataaaata ttctcaaaac tttttaacga gtgaaaaagt
       61 actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt acgaaattgg
      121 aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg taacgtctat
      181 tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt
      241 cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga ggtataaaat
      301 tgttgggagt attccttacc atttaagcac acaaattatt aaaaaagtgg tttttgaaag
      361 ccatgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta ccttggatat
      421 tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat tgcttaagct
      481 gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa aacttacccg
      541 ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg tttcaaaatg
      601 ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag caatgaaaca
      661 cgccaaagta aacaatttaa gtaccgttac ttatgagcaa gtattgtcta tttttaatag
      721 ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttgaatc tagagcggtt
      781 cagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atcttttgcc
      841 ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga aggttaagtc agttggggaa
      901 ctgcttaacc gtggtaactg gctttcgcag agcacagcaa ccaaatctgt ccttccagtg
      961 tagccggact ttggcgcaca cttcaagagc aaccgcgtgt ttagctaaac aaatcctctg
     1021 cgaactccca gttaccaatg gctgctgcca gtggcgtttt accgtgcttt tccgggttgg
     1081 actcaagtga acagttaccg gataaggcgc agcagtcggg ctgaacgggg agttcttgct
     1141 tacagcccag cttggagcga acgacctaca ccgagccgag ataccagtgt gtgagctatg
     1201 agaaagcgcc acacttcccg taagggagaa aggcggaaca ggtatccggt aaacggcagg
     1261 gtcggaacag gagagcgcaa gagggagcga cccgccggaa acggtgggga tctttaagtc
     1321 ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc ctcacggctc ccacagatgc
     1381 accggaaaag cgtctgttta tgtgaactct ggcaggaggg cggagcctat ggaaaaacgc
     1441 caccggcgcg gccctgctgt tttgcctcac atgttagtcc cctgcttatc cacggaatct
     1501 gtgggtaact ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg agcgtagcga
     1561 ccgagtgagc tagctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1621 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagggaagcg
     1681 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     1741 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     1801 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     1861 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     1921 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     1981 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     2041 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     2101 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     2161 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     2221 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     2281 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     2341 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     2401 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     2461 tgtctaacaa ttcgttcaag tacaagcact ttgggacgtt ctcccttagt gcttttttga
     2521 tttctcatag gccggcctgt tagtcatatg gacacttaag gcaaattgtt cagaactgaa
     2581 taaagctgac gttttgcttc tatcctttct ttgagtttta gtggataatg ataatgaaca
     2641 aggtgttcat aaatctatta taacaagaat tccaatcccg ggtaaagctt ccagcggccg
     2701 caggaggcaa aaatggataa gaaatactca ataggcttag atatcggcac aaatagcgtc
     2761 ggatgggcgg tgatcactga tgattataag gttccgtcta aaaagttcaa ggttctggga
     2821 aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga
     2881 gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag
     2941 aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt
     3001 ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat
     3061 cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat
     3121 catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg
     3181 gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct
     3241 gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt
     3301 gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg
     3361 agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc
     3421 ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt
     3481 gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat
     3541 aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta
     3601 tcagatgcta ttttactttc agatatccta agattaaata gtgaaataac taaggctccc
     3661 ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa
     3721 gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa
     3781 aacggatatg caggttatat tgatggggga gctagccaag aagaatttta taaatttatc
     3841 aaaccaattt tagaaaaaat ggatggtact gaggaattat tggcgaaact aaatcgtgaa
     3901 gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg
     3961 ggtgagctgc atgctattct gagaagacaa gaagactttt atccattttt aaaagacaat
     4021 cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg
     4081 cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg
     4141 aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca
     4201 aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag
     4261 tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaggg aatgcgaaaa
     4321 ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat
     4381 cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat
     4441 agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat
     4501 ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta
     4561 gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt
     4621 aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat
     4681 actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc
     4741 aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt tatgcagctg
     4801 atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa
     4861 ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt
     4921 attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca
     4981 gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat
     5041 tcgcgtgagc gtatgaaacg tattgaagaa ggtatcaaag aattaggaag tcagattctt
     5101 aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc
     5161 caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat
     5221 gatgtcgatc acattgttcc acaaagtttc attaaagacg attcaataga caataaggtc
     5281 ttaacgcgtt ctgataaaaa tcgtggtaaa tcgaataacg ttccaagtga agaagtagtc
     5341 aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag
     5401 tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa agctggtttt
     5461 atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca aattttggat
     5521 agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt
     5581 accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt
     5641 gagattaaca attaccatca tgcccatgat gcgtatctta atgccgtcgt tggaactgct
     5701 ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta taaagtttat
     5761 gatgttcgta aaatgattgc taagtctgag caggaaatag gcaaagcaac cgcaaaatat
     5821 ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag
     5881 attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa
     5941 gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag
     6001 aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa aagaaattcg
     6061 gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg ttttgatagt
     6121 ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag
     6181 ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc ctttgaaaaa
     6241 aatccgattg actttttaga agctaaagga tataaggaag ttagaaaaga cttaatcgtt
     6301 aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat gctggctagt
     6361 gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt gaatttttta
     6421 tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga acaaaaacaa
     6481 ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat cagtgaattt
     6541 tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc atataacaaa
     6601 catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg
     6661 aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat
     6721 acgtctacaa aagaagtttt agatgccact tttatccatc aatccatcac tggtctttat
     6781 gaaacacgca ttgatttgag tcagctagga ggtgactaag tcgacctcga gactagtcaa
     6841 ggtcggcaat tctgcagtac taggacgccg ccaagccagc ttaaacccag ctcaatgagc
     6901 tgggtttttt gtttgttaaa aatgaagaag aaactgtgaa gcgtatttag gtataagaga
     6961 cagccattct acagtttatt cttgacattg cactgtcccc ctggtataat aactatatga
     7021 gacctaggag gacaaacatg tcaaaaggag aagagctgtt cacaggtgtt gtgccgattc
     7081 tcgttgagct tgacggagat gtaaacggac acaaattctc tgttcgcggt gaaggtgaag
     7141 gagatgcaac aaacggcaag ctgacattga agtttatttg cacaactgga aagctgccgg
     7201 ttccttggcc gacacttgta acgacgctga cttacggcgt tcaatgcttc tctcgttatc
     7261 cagaccacat gaaacgccat gatttcttca aatctgcaat gcctgaaggc tacgttcaag
     7321 agcgtacgat cagcttcaaa gatgacggaa cgtacaaaac aagagcagaa gtgaagtttg
     7381 aaggtgacac acttgtgaac cgcattgaat tgaaaggcat tgatttcaaa gaagatggaa
     7441 acatccttgg acacaaactt gaatacaact tcaacagcca caacgtatac atcactgctg
     7501 acaaacaaaa aaacggcatc aaagcaaact tcaaaatccg tcataacgta gaggacggtt
     7561 ctgttcagct tgctgatcat tatcagcaaa atacaccgat cggtgacggc ccggttcttc
     7621 ttcctgataa ccattattta tcaactcaaa gcgtattatc aaaagaccca aatgaaaagc
     7681 gtgaccacat ggtgctgctt gaatttgtga cagctgctgg tatcactcac ggcatggatg
     7741 agctttataa gtaaggtctc ggtttaagag ctatgctgga aacagcatag caagtttaaa
     7801 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttctgtct
     7861 caatcgcaac atcaaaccaa aataaaaacc cccttcgact ttcgtcaggg ggcttttatt
     7921 tattcaataa tccctcctct caataaatct attgttgtac ttaattcaac ttccatttct
     7981 ctgtatcttt caatacgctc ttttaagtcc ttaatttctt tttttaattc ctcattttca
     8041 gcaaataact ctttttcttt gtttgtcatt ttatttcccc cgtttcagca tcaagaacct
     8101 ttgcataact tgctctatat ccacactgat aattgccctc aaaccataat ctaaaggcgc
     8161 tagagtttgt tgaaacaata tcttttacat cattcgtatt taaaattcca aactccgctc
     8221 ccctaaggcg aataaaagcc attaaatctt ttgtatttac caaattatag tcatccacta
     8281 tatctaaaag taaattcttc aattctcttt tttggctttc atcaagtgtt atatagcggt
     8341 caatatcaaa atcattaatg ttcaaaatat cttttttgtc gtatatatgt ttattcttag
     8401 caatagcgtc ctttgattca tgagtcaaat attcatatga acctttgata taatcaagta
     8461 tctcaacatg agcaactgaa ctattcccca attttcgctt aatcttgttc ctaacgcttt
     8521 ctattgttac aggatttcgt gcaatatata taacgtgata gtgtggtttt ttatagtgct
     8581 ttccattttg tataatatta ctattattcc atgtatcttt atcttttttt tcgtccatat
     8641 cgtgtaaagg actgacagcc atagatacgc ccaaactctc taatttttct ttccaatcat
     8701 taggaattga gtcaggatat aataaaaatc caaaatttct agctttagta tttttaatag
     8761 ccatgatata attaccttat caaaaacaag tagcgaaaac tcgtatcctt ctaaaaacgc
     8821 gagctttcgc ttattttttt tgttctgatt cctttcttgc atattcttct atagctaacg
     8881 ccgcaaccgc agattttgaa aaaccttttt gtttcgccat atctgttaat tttttatctt
     8941 gctcttttgt cagagaaatc ataactcttt ttttcgattc tgaaatcacc atttaaaaaa
     9001 ctccaatcaa ataattttat aaagttagtg tatcactttg taatcataaa aacaacaata
     9061 aagctactta aatatagatt tataaaaaac gttggcgaaa acgttggcga ttcgttggcg
     9121 attgaaaaac cccttaaacc cttgagccag ttgggataga gcgtttttgg cacaaaaatt
     9181 ggcactcggc acttaatggg gggtcgtagt acggaagcaa aattcgcttc ctttcccccc
     9241 atttttttcc aaattccaaa tttttttcaa aaattttcca gcgctaccgc tcggcaaaat
     9301 tgcaagcaat ttttaaaatc aaacccatga gggaatttca ttccctcata ctcccttgag
     9361 cctcctccaa ccgaaataga agggcgctgc gcttattatt tcattcagtc atcggctttc
     9421 ataatctaac agacaacatc ttcgctgcaa agccacgcta cgctcaaggg cttttacgct
     9481 acgataacgc ctgttttaac gattatgccg ataactaaac gaaataaacg ctaaaacgtc
     9541 tcagaaacga ttttgagacg ttttaataaa aaatcggata ataaatatat ataaacgtat
     9601 atagatttca taaagtctaa cacactagac ttatttactt cgtaattaag tcgttaaacc
     9661 gtgtgctcta cgaccaaaac tataaaacct ttaagaactt tcttttttta caagaaaaaa
     9721 gaaattagat aaatctctca tatcttttat tcaataatcg catccgattg cagtataaat
     9781 ttaacgatca ctcatcatgt tcatatttat caggatcctc tagagtcaca aaaagaaaaa
     9841 cgaaatgata caccaatcag tgcaaaaaaa gatataatgg gagataagac ggttcgtgtt
     9901 cgtgctgact tgcaccatat cataaaaatc gaaacagcaa agaatggcgg aaacgtaaaa
     9961 gaagttatgg aaataagact tagaagcaaa cttaagagtg tgttgatagt gcagtatctt
    10021 aaaattttgt ataataggaa ttgaagttaa attagatgct aaaaatttgt aattaagaag
//
