LOCUS       Exported               11375 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Episomally expresses KLF2 and NANOG in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCE-K2N
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11375)
  AUTHORS   Yoshimatsu S, Nakajima M, Iguchi A, Sanosaka T, Sato T, Nakamura M, 
            Nakajima R, Arai E, Ishikawa M, Imaizumi K, Watanabe H, Okahara J, 
            Noce T, Takeda Y, Sasaki E, Behr R, Edamura K, Shiozawa S, Okano H
  TITLE     Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts
            of Multiple Mammalian Species.
  JOURNAL   Stem Cell Reports. 2021 Mar 23. pii: S2213-6711(21)00132-6. doi: 
            10.1016/j.stemcr.2021.03.002.
  PUBMED    33798453
REFERENCE   2  (bases 1 to 11375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11375
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1731..1740
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2826..2891
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     primer_bind     complement(3819..3838)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3884..3939
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3938..3957)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4300..4316)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4300..4316)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4313..4335)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4324..4340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4348..4378)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4393..4414
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             4893..6818
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG?GGGA
                     GGAGGAGAGGGAGAGGGGGGAGAGGGAGGAGGAGAGGGAGAGGGGGAGGAGAGGGGAGG
                     GGGGGGGGGGGGGGGGGGGGGGGGGGGAGG?GAGGAGGAGAGGGGGAGGGGGGGGGGGG
                     GGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      7120..8909
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(9450..9467)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9621..10209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9701..9720)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10380..11240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11003..11022
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11241..11345)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc cggggggggg gggggggggg gggggggggg
      541 ggggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gccaccatgg
     1741 cgctgagtga acccatcctg ccgtccttct ccactttcgc cagcccgtgc cgcgagcgcg
     1801 gcctgcagga gcgctggccg cgcgccgaac ccgagtccgg cggcaccgac gacgacctca
     1861 acagcgtgct ggacttcatc ctgtccatgg ggctggatgg cctgggcgcc gaggccgccc
     1921 cggagccgcc gccgccgccc ccgccgcctg cgttctatta ccccgaaccc ggcgcgcccc
     1981 cgccctacag cgcccccgcg ggtggcctgg tgtctgagct gctgcgaccc gagctggatg
     2041 cgccgccggg gcccgcactg cacggccgct ttctgctggc gccgcccggc cgcctggtca
     2101 aggccgagcc ccctgaagcg gacggcggcg gcggctacgg ctgcgccccc gggctgaccc
     2161 gtggaccgcg cggcctcaag cgcgagggcg ccccgggccc ggcggcttcg tgcatgcgag
     2221 gtcccggggg ccgccccccg ccgccgcccg acacaccgcc gctcagcccc gacggccccg
     2281 cgcgcctgcc cgcgcccggt ccgcgcgcct ccttcccgcc gcctttcggt ggccctggtt
     2341 tcggcgcgcc cgggcccggc ctgcattacg cgccgcctgc gcccccagcc ttcggtctct
     2401 tcgacgacgc ggccgccgcc gcggcagccc tgggcctggc gccccccgcc gcccgcggtc
     2461 tcctcacgcc gcctgcgtcc ccgctggagc tgctggaggc caagccaaag cgcggccgcc
     2521 gctcttggcc ccgcaaacgc accgccactc acacctgcag ctacgcgggc tgcggcaaga
     2581 cctacaccaa gagttcgcat ctgaaggcgc atctgcgcac gcacacaggt gagaagccct
     2641 accactgcaa ctgggacggc tgcggctgga agtttgcgcg ctcagacgag ctcacgcgcc
     2701 actaccgaaa gcacacgggc caccggccat tccagtgcca tctgtgcgat cgtgccttct
     2761 cgcgctccga tcacctggcg ctgcacatga aacggcacat ggatcctcgc gctaagcggg
     2821 caccggtgaa acagactttg aattttgacc ttctcaagtt ggcgggagac gtggagtcca
     2881 acccagggcc ggatatcatg agtgtggatc cagcttgtcc ccaaagcttg ccttgctttg
     2941 aagcatccga ctgtaaagaa tcttcaccta tgcctgtgat ttgtgggcct gaagaaaact
     3001 atccatcctt gcaaatgtct tctgctgaga tgcctcacac ggagactgtc tctcctcttc
     3061 cctcctccat ggatctgctt attcaggaca gccctgattc ttccaccagt cccaaaggca
     3121 aacaacccac ttctgcagag aatagtgtcg caaaaaagga agacaaggtc ccagtcaaga
     3181 aacagaagac cagaactgtg ttctcttcca cccagctgtg tgtactcaat gatagatttc
     3241 agagacagaa atacctcagc ctccagcaga tgcaagaact ctccaacatc ctgaacctca
     3301 gctacaaaca ggtgaagacc tggttccaga accagagaat gaaatctaag aggtggcaga
     3361 aaaacaactg gccgaagaat agcaatggtg tgacgcagaa ggcctcagca cctacctacc
     3421 ccagcctcta ctcttcctac caccagggat gcctggtgaa cccgactggg aaccttccaa
     3481 tgtggagcaa ccagacctgg aacaattcaa cctggagcaa ccagacccag aacatccagt
     3541 cctggagcaa ccactcctgg aacactcaga cctggtgcac ccaatcctgg aacaatcagg
     3601 cctggaacag tcccttctat aactgtggag aggaatctct gcagtcctgc atgcagttcc
     3661 agccaaattc tcctgccagt gacttggagg ctgctttgga agctgctggg gaaggcctta
     3721 atgtaataca gcagaccact aggtatttta gtactccaca aaccatggat ttattcctaa
     3781 actactccat gaacatgcaa cctgaagacg tgtgaagatc tttttccctc tgccaaaaat
     3841 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     3901 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     3961 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     4021 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     4081 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     4141 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     4201 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     4261 agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     4321 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     4381 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4441 ccagtcggga aacctgtcgt gccagcggat ctcaattctc atgtttgaca gcttatcatc
     4501 gataagctga tcctcacagg ccgcacccag cttttcttcc gttgccccag tagcatctct
     4561 gtctggtgac cttgaagagg aagaggaggg gtcccgagaa tccccatccc taccgtccag
     4621 caaaaagggg gacgaggaat ttgaggcctg gcttgaggct caggacgcaa atcttgagga
     4681 tgttcagcgg gagttttccg ggctgcgagt aattggtgat gaggacgagg atggttcgga
     4741 ggatggggaa ttttcagacc tggatctgtc tgacagcgac catgaagggg atgagggtgg
     4801 gggggctgtt ggagggggca ggagtctgca ctccctgtat tcactgagcg tcgtctaata
     4861 aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca ggtacaggac
     4921 ctggaaatgg cctaggagag aagggagaca catctggacc agaaggctcc ggcggcagtg
     4981 gacctcaaag aagagggggt gataaccatg gacgaggacg gggaagagga cgaggacgag
     5041 gaggcggaag accaggagcc ccgggcggct caggatcagg gccaagacat agagatggtg
     5101 tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa agggacccac ggtggaacag
     5161 gagcaggagc aggagcggga ggggcaggag caggaggggc aggagcagga ggaggggcag
     5221 gagcaggagg aggggcagga ggggcaggag ggggaggagg ggcaggagga ggaggagggg
     5281 gaggaggagg aggaggggga ggagggggag gagggggagg aggaggagga gggggaggag
     5341 gaggaggagg gggaggaggg ggaggagsag gaggaggggc aggaggggca ggaggggcag
     5401 gagcaggagg aggggcagga gcaggaggag ggggaggagg ggcaggagca ggaggagggg
     5461 caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggaggg ggaggagggg
     5521 caggaggggc aggagcagga gggggaggag caggaggagg gggaggaggg ggaggagggg
     5581 gaggaggagg agggggagga ggaggagggg gaggaggagg agggggagga ggaggagggg
     5641 caggaggggs aggagcagga ggggcaggag gggcaggagc aggaggggga ggaggggcag
     5701 gaggaggagg agggggagga gggggaggag gaggaggagg gggaggaggg ggaggaggag
     5761 gagggggagg agggggagga ggaggagggg gaggaggggg aggaggagga gggggaggag
     5821 gggcaggagc aggaggaggg gcaggagcag gaggggcagg agcaggaggt ggaggccggg
     5881 gtcgaggagg cagtggaggc cggggtcgag gaggtagtgg aggccggggt cgaggaggta
     5941 gtggaggccg ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt gaaagagcca
     6001 gggggagagg tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt cagtcatcat
     6061 catccgggtc tccaccgcgc aggccccctc caggtagaag gccatttttc caccctgtag
     6121 gggaagccga ttattttgaa taccaccaag aaggtggccc agatggtgag cctgacgtgc
     6181 ccccgggagc gatagagcag ggccccgcag atgacccagg agaaggccca agcactggac
     6241 cccggggtca gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga aagcatcgtg
     6301 gtcaaggagg ttccaacccg aaatttgaga acattgcaga aggtttaaga gctctcctgg
     6361 ctaggagtca cgtagaaagg actaccgacg aaggaacttg ggtcgccggt gtgttcgtat
     6421 atggaggtag taagacctcc ctttacaacc taaggcgagg aactgccctt gctattccac
     6481 aatgtcgtct tacaccattg agtcgtctcc cctttggaat ggcccctgga cccggcccac
     6541 aacctggccc gctaagggag tccattgtct gttatttcat ggtcttttta caaactcata
     6601 tatttgctga ggttttgaag gatgcgatta aggaccttgt tatgacaaag cccgctccta
     6661 cctgcaatat cagggtgact gtgtgcagct ttgacgatgg agtagatttg cctccctggt
     6721 ttccacctat ggtggaaggg gctgccgcgg agggtgatga cggagatgac ggagatgaag
     6781 gaggtgatgg agatgagggt gaggaagggc aggagtgatg taacttgtta ggagacgccc
     6841 tcaatcgtat taaaagccgt gtattccccc gcactaaaga ataaatcccc agtagacatc
     6901 atgcgtgctg ttggtgtatt tctggccatc tgtcttgtca ccattttcgt cctcccaaca
     6961 tggggcaatt gggcataccc atgttgtcac gtcactcagc tccgcgctca acaccttctc
     7021 gcgttggaaa acattagcga catttacctg gtgagcaatc agacatgcga cggctttagc
     7081 ctggcctcct taaattcacc taagaatggg agcaaccagc aggaaaagga caagcagcga
     7141 aaattcacgc ccccttggga ggtggcggca tatgcaaagg atagcactcc cactctacta
     7201 ctgggtatca tatgctgact gtatatgcat gaggatagca tatgctaccc ggatacagat
     7261 taggatagca tatactaccc agatatagat taggatagca tatgctaccc agatatagat
     7321 taggatagcc tatgctaccc agatataaat taggatagca tatactaccc agatatagat
     7381 taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat
     7441 taggatagca tatgctaccc agatatagat taggatagca tatgctatcc agatatttgg
     7501 gtagtatatg ctacccagat ataaattagg atagcatata ctaccctaat ctctattagg
     7561 atagcatatg ctacccggat acagattagg atagcatata ctacccagat atagattagg
     7621 atagcatatg ctacccagat atagattagg atagcctatg ctacccagat ataaattagg
     7681 atagcatata ctacccagat atagattagg atagcatatg ctacccagat atagattagg
     7741 atagcctatg ctacccagat atagattagg atagcatatg ctatccagat atttgggtag
     7801 tatatgctac ccatggcaac attagcccac cgtgctctca gcgacctcgt gaatatgagg
     7861 accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg taatttgtcc tccagatcgc
     7921 agcaatcgcg cccctatctt ggcccgccca cctacttatg caggtattcc ccggggtgcc
     7981 attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt agcggggtta caatcagcca
     8041 agttattaca cccttatttt acagtccaaa accgcagggc ggcgtgtggg ggctgacgcg
     8101 tgcccccact ccacaatttc aaaaaaaaga gtggccactt gtctttgttt atgggcccca
     8161 ttggcgtgga gccccgttta attttcgggg gtgttagaga caaccagtgg agtccgctgc
     8221 tgtcggcgtc cactctcttt ccccttgtta caaatagagt gtaacaacat ggttcacctg
     8281 tcttggtccc tgcctgggac acatcttaat aaccccagta tcatattgca ctaggattat
     8341 gtgttgccca tagccataaa ttcgtgtgag atggacatcc agtctttacg gcttgtcccc
     8401 accccatgga tttctattgt taaagatatt cagaatgttt cattcctaca ctagtattta
     8461 ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc acaatgccac cactgaaccc
     8521 cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt gttttcgagc acctcacata
     8581 caccttactg ttcacaactc agcagttatt ctattagcta aacgaaggag aatgaagaag
     8641 caggcgaaga ttcaggagag ttcactgccc gctccttgat cttcagccac tgcccttgtg
     8701 actaaaatgg ttcactaccc tcgtggaatc ctgaccccat gtaaataaaa ccgtgacagc
     8761 tcatggggtg ggagatatcg ctgttcctta ggaccctttt actaacccta attcgatagc
     8821 atatgcttcc cgttgggtaa catatgctat tgaattaggg ttagtctgga tagtatatac
     8881 tactacccgg gaagcatatg ctacccgttt agggttaaca agggggcctt ataaacacta
     8941 ttgctaatgc cctcttgagg gtccgcttat cggtagctac acaggcccct ctgattgacg
     9001 ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg tacatgtccc ccagcattgg
     9061 tgtaagagct tcagccaaga gttacacata aaggcaatgt tgtgttgcag tccacagact
     9121 gcaaagtctg ctccaggatg aaagccactc agtgttggca aatgtgcaca tccatttata
     9181 aggatgtcaa ctacagtcag agaacccctt tgtgtttggt ccccccccgt gtcacatgtg
     9241 gaacagggcc cagttggcaa gttgtaccaa ccaactgaag ggattacatg cactgccccg
     9301 cgaagaaggg gcagagatgt cgtagtcagg tttagttcgt ccggggcggg gatcgatcct
     9361 ctagagtcga cgctagcgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc
     9421 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9481 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9541 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9601 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9661 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9721 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9781 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9841 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9901 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9961 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10021 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    10081 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10141 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10201 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10261 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10321 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10381 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    10441 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10501 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10561 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10621 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10681 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10741 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10801 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10861 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10921 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10981 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11041 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    11101 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    11161 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    11221 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    11281 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11341 ccgcgcacat ttccccgaaa agtgccacct gggtc
//
