LOCUS Exported 11215 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses human ACE2 in mammalian cells. ACCESSION . VERSION . KEYWORDS pLenti-hACE2-hygro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11215) AUTHORS Daniloski Z, Jordan TX, Wessels HH, Hoagland DA, Kasela S, Legut M, Maniatis S, Mimitou EP, Lu L, Geller E, Danziger O, Rosenberg BR, Phatnani H, Smibert P, Lappalainen T, tenOever BR, Sanjana NE TITLE Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells. JOURNAL Cell. 2020 Oct 24. pii: S0092-8674(20)31394-5. doi: 10.1016/j.cell.2020.10.030. PUBMED 33147445 REFERENCE 2 (bases 1 to 11215) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11215 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 96..1274 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 327..1265 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1222..1242 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 3736..3792 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" misc_feature 3813..4401 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3866..3886) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4284..4295) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4473..4706 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 4784..4905 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4821..4840) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4875..4894 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 4945..5080 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(5100..5119) /label=T7 /note="T7 promoter, forward primer" promoter complement(5101..5119) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5129..5146) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(5129..5145) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5138..5160) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 5287..5742 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5374..5393) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5584..5605 /label=F1ori-F /note="F1 origin, forward primer" promoter 5768..5872 /gene="bla" /label=AmpR promoter CDS 5873..6733 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6091..6110) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6904..7492 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7393..7412 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7646..7663 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7780..7801 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7816..7846 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7854..7870 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7859..7881 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7878..7894 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7878..7894 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7913..7933 /label=T3 /note="T3 promoter, forward primer" promoter 7915..7933 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 7961..8187 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 8188..8368 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 8415..8540 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9033..9266 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9451..9495 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 9726..9936 /label=SV40 promoter /note="SV40 early promoter" promoter 9748..9944 /label=SV40 promoter /note="SV40 early promoter" rep_origin 9795..9930 /label=SV40 ori /note="SV40 origin of replication" CDS 9966..10985 /codon_start=1 /product="hygromycin B phosphotransferase" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK " misc_feature 11056..11173 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct 61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag 121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg 181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc 361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga 421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag 481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc 541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa 601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc 661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt 721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg 781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc 841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc 901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg 961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact 1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt 1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg 1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg 1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat 1261 ttcaggtgtc gtgaggccac catgtcaagc tcttcctggc tccttctcag ccttgttgct 1321 gtaactgctg ctcagtccac cattgaggaa caggccaaga catttttgga caagtttaac 1381 cacgaagccg aagacctgtt ctatcaaagt tcacttgctt cttggaatta taacaccaat 1441 attactgaag agaatgtcca aaacatgaat aatgctgggg acaaatggtc tgccttttta 1501 aaggaacagt ccacacttgc ccaaatgtat ccactacaag aaattcagaa tctcacagtc 1561 aagcttcagc tgcaggctct tcagcaaaat gggtcttcag tgctctcaga agacaagagc 1621 aaacggttga acacaattct aaatacaatg agcaccatct acagtactgg aaaagtttgt 1681 aacccagata atccacaaga atgcttatta cttgaaccag gtttgaatga aataatggca 1741 aacagtttag actacaatga gaggctctgg gcttgggaaa gctggagatc tgaggtcggc 1801 aagcagctga ggccattata tgaagagtat gtggtcttga aaaatgagat ggcaagagca 1861 aatcattatg aggactatgg ggattattgg agaggagact atgaagtaaa tggggtagat 1921 ggctatgact acagccgcgg ccagttgatt gaagatgtgg aacatacctt tgaagagatt 1981 aaaccattat atgaacatct tcatgcctat gtgagggcaa agttgatgaa tgcctatcct 2041 tcctatatca gtccaattgg atgcctccct gctcatttgc ttggtgatat gtggggtaga 2101 ttttggacaa atctgtactc tttgacagtt ccctttggac agaaaccaaa catagatgtt 2161 actgatgcaa tggtggacca ggcctgggat gcacagagaa tattcaagga ggccgagaag 2221 ttctttgtat ctgttggtct tcctaatatg actcaaggat tctgggaaaa ttccatgcta 2281 acggacccag gaaatgttca gaaagcagtc tgccatccca cagcttggga cctggggaag 2341 ggcgacttca ggatccttat gtgcacaaag gtgacaatgg acgacttcct gacagctcat 2401 catgagatgg ggcatatcca gtatgatatg gcatatgctg cacaaccttt tctgctaaga 2461 aatggagcta atgaaggatt ccatgaagct gttggggaaa tcatgtcact ttctgcagcc 2521 acacctaagc atttaaaatc cattggtctt ctgtcacccg attttcaaga agacaatgaa 2581 acagaaataa acttcctgct caaacaagca ctcacgattg ttgggactct gccatttact 2641 tacatgttag agaagtggag gtggatggtc tttaaagggg aaattcccaa agaccagtgg 2701 atgaaaaagt ggtgggagat gaagcgagag atagttgggg tggtggaacc tgtgccccat 2761 gatgaaacat actgtgaccc cgcatctctg ttccatgttt ctaatgatta ctcattcatt 2821 cgatattaca caaggaccct ttaccaattc cagtttcaag aagcactttg tcaagcagct 2881 aaacatgaag gccctctgca caaatgtgac atctcaaact ctacagaagc tggacagaaa 2941 ctgttcaata tgctgaggct tggaaaatca gaaccctgga ccctagcatt ggaaaatgtt 3001 gtaggagcaa agaacatgaa tgtaaggcca ctgctcaact actttgagcc cttatttacc 3061 tggctgaaag accagaacaa gaattctttt gtgggatgga gtaccgactg gagtccatat 3121 gcagaccaaa gcatcaaagt gaggataagc ctaaaatcag ctcttggaga taaagcatat 3181 gaatggaacg acaatgaaat gtacctgttc cgatcatctg ttgcatatgc tatgaggcag 3241 tactttttaa aagtaaaaaa tcagatgatt ctttttgggg aggaggatgt gcgagtggct 3301 aatttgaaac caagaatctc ctttaatttc tttgtcactg cacctaaaaa tgtgtctgat 3361 atcattccta gaactgaagt tgaaaaggcc atcaggatgt cccggagccg tatcaatgat 3421 gctttccgtc tgaatgacaa cagcctagag tttctgggga tacagccaac acttggacct 3481 cctaaccagc cccctgtttc catatggctg attgtttttg gagttgtgat gggagtgata 3541 gtggttggca ttgtcatcct gatcttcact gggatcagag atcggaagaa gaaaaataaa 3601 gcaagaagtg gagaaaatcc ttatgcctcc atcgatatta gcaaaggaga aaataatcca 3661 ggattccaaa acactgatga tgttcagacc tcctttggta ccgagacctc ccaggtggcg 3721 cccgctggat ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag 3781 aatcctggac cgtaaccacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg 3841 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 3901 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 3961 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 4021 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 4081 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 4141 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 4201 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 4261 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 4321 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 4381 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga 4441 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 4501 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 4561 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 4621 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 4681 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca 4741 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 4801 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 4861 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 4921 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 4981 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 5041 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc 5101 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg 5161 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 5221 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 5281 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 5341 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 5401 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 5461 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 5521 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 5581 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 5641 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 5701 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg 5761 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 5821 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 5881 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 5941 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 6001 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 6061 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 6121 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 6181 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 6241 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 6301 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 6361 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 6421 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 6481 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 6541 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 6601 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 6661 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 6721 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 6781 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 6841 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 6901 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 6961 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 7021 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 7081 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 7141 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 7201 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 7261 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 7321 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 7381 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 7441 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 7501 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 7561 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 7621 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc 7681 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 7741 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 7801 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 7861 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa 7921 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc 7981 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag 8041 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa 8101 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta 8161 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg 8221 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 8281 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 8341 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag 8401 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag 8461 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga 8521 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa 8581 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca 8641 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 8701 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat 8761 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg 8821 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg 8881 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat 8941 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga 9001 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga 9061 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta 9121 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat 9181 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa 9241 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc 9301 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat 9361 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc 9421 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat 9481 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta 9541 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata 9601 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg 9661 aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 9721 ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc 9781 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 9841 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 9901 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta 9961 ccaccatgaa aaagcctgaa ctcaccgcta cctctgtcga gaagtttctg atcgaaaagt 10021 tcgacagcgt ctccgacctg atgcagctct ccgagggcga agaatctcgg gctttcagct 10081 tcgatgtggg agggcgtgga tatgtcctgc gggtgaatag ctgcgccgat ggtttctaca 10141 aagatcgcta tgtttatcgg cactttgcat ccgccgctct ccctattccc gaagtgcttg 10201 acattgggga gttcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca 10261 ccttgcaaga cctgcctgaa accgaactgc ccgctgttct ccagcccgtc gccgaggcca 10321 tggatgccat cgctgccgcc gatcttagcc agaccagcgg gttcggccca ttcggacctc 10381 aaggaatcgg tcaatacact acatggcgcg atttcatctg cgctattgct gatccccatg 10441 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc ctccgtcgcc caggctctcg 10501 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgccgatt 10561 tcggctccaa caatgtcctg accgacaatg gccgcataac agccgtcatt gactggagcg 10621 aggccatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccctggt 10681 tggcttgtat ggagcagcag acccgctact tcgagcggag gcatcccgag cttgcaggat 10741 ctcctcggct ccgggcttat atgctccgca ttggtcttga ccaactctat cagagcttgg 10801 ttgacggcaa tttcgatgat gcagcttggg ctcagggtcg ctgcgacgca atcgtccggt 10861 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgctgcc gtctggaccg 10921 atggctgtgt ggaagtgctc gccgatagtg gaaacagacg ccccagcact cgtcctaggg 10981 caaaggatct gcagtaatga ggcgcgccgt taacgaattc tagatcttga gacaaatggc 11041 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag 11101 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa 11161 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc gccgg //