LOCUS Exported 11877 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Non-integrating (episomal) expression of human SOX2 and KLF4. ACCESSION . VERSION . KEYWORDS pCE-hSK SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11877) AUTHORS Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S TITLE An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells. JOURNAL Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293. PUBMED 23193063 REFERENCE 2 (bases 1 to 11877) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11877 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 382..659 /label=chicken beta-actin promoter intron 660..1676 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1600..1622 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1684..1703 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS 2716..2781 /codon_start=1 /product="2A peptide from foot-and-mouth disease virus polyprotein" /label=F2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="VKQTLNFDLLKLAGDVESNPGP" primer_bind complement(4321..4340) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 4386..4441 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(4440..4459) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(4802..4818) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4802..4818) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4815..4837) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4826..4842 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4850..4880) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4895..4916 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." CDS 5395..7320 /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGG?G?GGGAGGAG?GGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" rep_origin 7622..9411 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" primer_bind complement(9952..9969) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10123..10711) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10203..10222) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10882..11742) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11505..11524 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11743..11847) /gene="bla" /label=AmpR promoter ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct 421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga 481 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg 541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc 601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg 661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc 721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc 781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa 841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg 901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct 961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg 1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt 1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc 1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg 1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg 1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg 1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc 1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc 1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg 1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc 1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga 1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc 1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gcccttcacc 1741 atgtacaaca tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc 1801 ggcggcggca actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc 1861 gtcaagcggc ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc 1921 caggagaacc ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa 1981 cttttgtcgg agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg 2041 cacatgaagg agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg 2101 aagaaggata agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg 2161 agcggggtcg gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac 2221 gcgcacatga acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac 2281 ccgcagcacc cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac 2341 gacgtgagcg ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg 2401 cccacctaca gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg 2461 ggttcggtgg tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac 2521 tccagggcgc cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc 2581 gccgaggtgc cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc 2641 ggcccggtgc ccggcacggc cattaacggc acactgcccc tctcacacat gggggatctc 2701 aaaattgtcg ctcctgtcaa acaaactctt aactttgatt tactcaaact ggctggggat 2761 gtagaaagca atccaggtcc actcatgaac aattcgccct tcaccatggc tgtcagtgac 2821 gcgctgctcc catctttctc cacgttcgcg tctggcccgg cgggaaggga gaagacactg 2881 cgtcaagcag gtgccccgaa taaccgctgg cgggaggagc tctcccacat gaagcgactt 2941 cccccagtgc ttcccggccg cccctatgac ctggcggcgg cgaccgtggc cacagacctg 3001 gagagcggcg gagccggtgc ggcttgcggc ggtagcaacc tggcgcccct acctcggaga 3061 gagaccgagg agttcaacga tctcctggac ctggacttta ttctctccaa ttcgctgacc 3121 catcctccgg agtcagtggc cgccaccgtg tcctcgtcag cgtcagcctc ctcttcgtcg 3181 tcgccgtcga gcagcggccc tgccagcgcg ccctccacct gcagcttcac ctatccgatc 3241 cgggccggga acgacccggg cgtggcgccg ggcggcacgg gcggaggcct cctctatggc 3301 agggagtccg ctccccctcc gacggctccc ttcaacctgg cggacatcaa cgacgtgagc 3361 ccctcgggcg gcttcgtggc cgagctcctg cggccagaat tggacccggt gtacattccg 3421 ccgcagcagc cgcagccgcc aggtggcggg ctgatgggca agttcgtgct gaaggcgtcg 3481 ctgagcgccc ctggcagcga gtacggcagc ccgtcggtca tcagcgtcag caaaggcagc 3541 cctgacggca gccacccggt ggtggtggcg ccctacaacg gcgggccgcc gcgcacgtgc 3601 cccaagatca agcaggaggc ggtctcttcg tgcacccact tgggcgctgg accccctctc 3661 agcaatggcc accggccggc tgcacacgac ttccccctgg ggcggcagct ccccagcagg 3721 actaccccga ccctgggtct tgaggaagtg ctgagcagca gggactgtca ccctgccctg 3781 ccgcttcctc ccggcttcca tccccacccg gggcccaatt acccatcctt cctgcccgat 3841 cagatgcagc cgcaagtccc gccgctccat taccaagagc tcatgccacc cggttcctgc 3901 atgccagagg agcccaagcc aaagagggga agacgatcgt ggccccggaa aaggaccgcc 3961 acccacactt gtgattacgc gggctgcggc aaaacctaca caaagagttc ccatctcaag 4021 gcacacctgc gaacccacac aggtgagaaa ccttaccact gtgactggga cggctgtgga 4081 tggaaattcg cccgctcaga tgaactgacc aggcactacc gtaaacacac ggggcaccgc 4141 ccgttccagt gccaaaaatg cgaccgagca ttttccaggt cggaccacct cgccttacac 4201 atgaagagac atttttaaaa gggcgaattg cctgcaggaa ttcactcctc aggtgcaggc 4261 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga 4321 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc 4381 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc 4441 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta 4501 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg 4561 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac 4621 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa 4681 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat 4741 agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat 4801 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 4861 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 4921 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atctcaattc 4981 tcatgtttga cagcttatca tcgataagct gatcctcaca ggccgcaccc agcttttctt 5041 ccgttgcccc agtagcatct ctgtctggtg accttgaaga ggaagaggag gggtcccgag 5101 aatccccatc cctaccgtcc agcaaaaagg gggacgagga atttgaggcc tggcttgagg 5161 ctcaggacgc aaatcttgag gatgttcagc gggagttttc cgggctgcga gtaattggtg 5221 atgaggacga ggatggttcg gaggatgggg aattttcaga cctggatctg tctgacagcg 5281 accatgaagg ggatgagggt gggggggctg ttggaggggg caggagtctg cactccctgt 5341 attcactgag cgtcgtctaa taaagatgtc tattgatctc ttttagtgtg aatcatgtct 5401 gacgaggggc caggtacagg acctggaaat ggcctaggag agaagggaga cacatctgga 5461 ccagaaggct ccggcggcag tggacctcaa agaagagggg gtgataacca tggacgagga 5521 cggggaagag gacgaggacg aggaggcgga agaccaggag ccccgggcgg ctcaggatca 5581 gggccaagac atagagatgg tgtccggaga ccccaaaaac gtccaagttg cattggctgc 5641 aaagggaccc acggtggaac aggagcagga gcaggagcgg gaggggcagg agcaggaggg 5701 gcaggagcag gaggaggggc aggagcagga ggaggggcag gaggggcagg aggggcagga 5761 ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggaggggca 5821 ggagcaggag gaggggsagg agsaggagga ggggcaggag gggcaggags aggaggaggg 5881 gcaggagggg caggaggggc aggagcagga ggaggggcag gagcaggagg aggggcagga 5941 ggggcaggag caggaggagg ggcaggaggg gcaggagggg caggagcagg aggaggggca 6001 ggagcaggag gggcaggagg ggcaggaggg gcaggagcag gaggggcagg agcaggagga 6061 ggggcaggag gggcaggagg ggcaggagca ggaggggcag gagcaggagg ggcaggagca 6121 ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga 6181 gcaggagggg caggaggggc aggagcagga ggaggggcag gaggggcagg agcaggagga 6241 ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg ggcaggaggg 6301 gcaggagcag gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggggca 6361 ggagcaggag gtggaggccg gggtcgagga ggcagtggag gccggggtcg aggaggtagt 6421 ggaggccggg gtcgaggagg tagtggaggc cgccggggta gaggacgtga aagagccagg 6481 gggggaagtc gtgaaagagc cagggggaga ggtcgtggac gtggagaaaa gaggcccagg 6541 agtcccagta gtcagtcatc atcatccggg tctccaccgc gcaggccccc tccaggtaga 6601 aggccatttt tccaccctgt aggggaagcc gattattttg aataccacca agaaggtggc 6661 ccagatggtg agcctgacgt gcccccggga gcgatagagc agggccccgc agatgaccca 6721 ggagaaggcc caagcactgg accccggggt cagggtgatg gaggcaggcg caaaaaagga 6781 gggtggtttg gaaagcatcg tggtcaagga ggttccaacc cgaaatttga gaacattgca 6841 gaaggtttaa gagctctcct ggctaggagt cacgtagaaa ggactaccga cgaaggaact 6901 tgggtcgccg gtgtgttcgt atatggaggt agtaagacct ccctttacaa cctaaggcga 6961 ggaactgccc ttgctattcc acaatgtcgt cttacaccat tgagtcgtct cccctttgga 7021 atggcccctg gacccggccc acaacctggc ccgctaaggg agtccattgt ctgttatttc 7081 atggtctttt tacaaactca tatatttgct gaggttttga aggatgcgat taaggacctt 7141 gttatgacaa agcccgctcc tacctgcaat atcagggtga ctgtgtgcag ctttgacgat 7201 ggagtagatt tgcctccctg gtttccacct atggtggaag gggctgccgc ggagggtgat 7261 gacggagatg acggagatga aggaggtgat ggagatgagg gtgaggaagg gcaggagtga 7321 tgtaacttgt taggagacgc cctcaatcgt attaaaagcc gtgtattccc ccgcactaaa 7381 gaataaatcc ccagtagaca tcatgcgtgc tgttggtgta tttctggcca tctgtcttgt 7441 caccattttc gtcctcccaa catggggcaa ttgggcatac ccatgttgtc acgtcactca 7501 gctccgcgct caacaccttc tcgcgttgga aaacattagc gacatttacc tggtgagcaa 7561 tcagacatgc gacggcttta gcctggcctc cttaaattca cctaagaatg ggagcaacca 7621 gcaggaaaag gacaagcagc gaaaattcac gcccccttgg gaggtggcgg catatgcaaa 7681 ggatagcact cccactctac tactgggtat catatgctga ctgtatatgc atgaggatag 7741 catatgctac ccggatacag attaggatag catatactac ccagatatag attaggatag 7801 catatgctac ccagatatag attaggatag cctatgctac ccagatataa attaggatag 7861 catatactac ccagatatag attaggatag catatgctac ccagatatag attaggatag 7921 cctatgctac ccagatatag attaggatag catatgctac ccagatatag attaggatag 7981 catatgctat ccagatattt gggtagtata tgctacccag atataaatta ggatagcata 8041 tactacccta atctctatta ggatagcata tgctacccgg atacagatta ggatagcata 8101 tactacccag atatagatta ggatagcata tgctacccag atatagatta ggatagccta 8161 tgctacccag atataaatta ggatagcata tactacccag atatagatta ggatagcata 8221 tgctacccag atatagatta ggatagccta tgctacccag atatagatta ggatagcata 8281 tgctatccag atatttgggt agtatatgct acccatggca acattagccc accgtgctct 8341 cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg cgcaagtgtg 8401 tgtaatttgt cctccagatc gcagcaatcg cgcccctatc ttggcccgcc cacctactta 8461 tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc aagtggtttg accgcagtgg 8521 ttagcggggt tacaatcagc caagttatta cacccttatt ttacagtcca aaaccgcagg 8581 gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa gagtggccac 8641 ttgtctttgt ttatgggccc cattggcgtg gagccccgtt taattttcgg gggtgttaga 8701 gacaaccagt ggagtccgct gctgtcggcg tccactctct ttccccttgt tacaaataga 8761 gtgtaacaac atggttcacc tgtcttggtc cctgcctggg acacatctta ataaccccag 8821 tatcatattg cactaggatt atgtgttgcc catagccata aattcgtgtg agatggacat 8881 ccagtcttta cggcttgtcc ccaccccatg gatttctatt gttaaagata ttcagaatgt 8941 ttcattccta cactagtatt tattgcccaa ggggtttgtg agggttatat tggtgtcata 9001 gcacaatgcc accactgaac cccccgtcca aattttattc tgggggcgtc acctgaaacc 9061 ttgttttcga gcacctcaca tacaccttac tgttcacaac tcagcagtta ttctattagc 9121 taaacgaagg agaatgaaga agcaggcgaa gattcaggag agttcactgc ccgctccttg 9181 atcttcagcc actgcccttg tgactaaaat ggttcactac cctcgtggaa tcctgacccc 9241 atgtaaataa aaccgtgaca gctcatgggg tgggagatat cgctgttcct taggaccctt 9301 ttactaaccc taattcgata gcatatgctt cccgttgggt aacatatgct attgaattag 9361 ggttagtctg gatagtatat actactaccc gggaagcata tgctacccgt ttagggttaa 9421 caagggggcc ttataaacac tattgctaat gccctcttga gggtccgctt atcggtagct 9481 acacaggccc ctctgattga cgttggtgta gcctcccgta gtcttcctgg gcccctggga 9541 ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa gagttacaca taaaggcaat 9601 gttgtgttgc agtccacaga ctgcaaagtc tgctccagga tgaaagccac tcagtgttgg 9661 caaatgtgca catccattta taaggatgtc aactacagtc agagaacccc tttgtgtttg 9721 gtcccccccc gtgtcacatg tggaacaggg cccagttggc aagttgtacc aaccaactga 9781 agggattaca tgcactgccc cgcgaagaag gggcagagat gtcgtagtca ggtttagttc 9841 gtccggggcg gggatcgatc ctctagagtc gacgctagcg gatccgctgc attaatgaat 9901 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 9961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 10021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 10081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 10141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 10201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 10261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 10321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 10381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 10441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 10501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 10561 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 10621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 10681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 10741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 10801 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 10861 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 10921 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 10981 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 11041 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 11101 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 11161 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 11221 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 11281 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 11341 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 11401 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 11461 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 11521 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 11581 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 11641 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 11701 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 11761 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 11821 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtc //