LOCUS Exported 9962 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION QUAS reporter line that expresses cytosolic dTomato and the rat TRPV1 ion channel, separated by T2A. ACCESSION . VERSION . KEYWORDS 15x-QUAS-dTomato-T2A-TRPV1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9962) TITLE JoveVosshall_2020 JOURNAL Unpublished REFERENCE 2 (bases 1 to 9962) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9962 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 5..243 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" primer_bind 190..211 /label=pCasper-hs /note="Drosophila Hsp70 promoter, forward primer" CDS 319..1020 /codon_start=1 /product="dimeric variant of DsRed fluorescent protein (Shaner et al., 2004)" /label=dTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLYGMDELYK" primer_bind complement(452..470) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 720..739 /label=mCherry-F /note="mCherry, forward primer" primer_bind 914..937 /label=DsRed1-C /note="DsRed1, forward primer" CDS 1024..1077 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" intron 3748..3813 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 3943..3963 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 4235..4369 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" repeat_region 4644..4678 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" primer_bind complement(5169..5185) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5169..5185) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5182..5204) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5193..5209 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5217..5247) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5262..5283 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5400..5417) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5571..6159) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5651..5670) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6330..7190) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6953..6972 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7191..7295) /gene="bla" /label=AmpR promoter rep_origin complement(7321..7776) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7458..7479) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 7670..7689 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7903..7925 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 7917..7934 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 7918..7934 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" repeat_region 8064..8126 /label=piggyBac right (3') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" regulatory 8609..8618 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 8615..9334 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(8660..8681) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(8921..8940) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 9268..9289 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 9457..9578 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" ORIGIN 1 agcgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc 61 aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta 121 aacaatctgc agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt 181 aaatcaactg caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact 241 ctgaataggg aattgggaat tcgttaacag atcgggggat caattcgtta acagatctgc 301 ggccgcggct cgagcaaaat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc 361 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc 421 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc 481 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg 541 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg 601 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg 661 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc 721 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc 781 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccgctac 841 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac 901 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag 961 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag 1021 ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 1081 gaacaacggg ctagcttaga ctcagaggag tctgagtccc caccccaaga gaactcctgc 1141 ctggaccctc cagacagaga ccctaactgc aagccacctc cagtcaagcc ccacatcttc 1201 actaccagga gtcgtacccg gctttttggg aagggtgact cggaggaggc ctctcccctg 1261 gactgccctt atgaggaagg cgggctggct tcctgcccta tcatcactgt cagctctgtt 1321 ctaactatcc agaggcctgg ggatggacct gccagtgtca ggccgtcatc ccaggactcc 1381 gtctccgctg gtgagaagcc cccgaggctc tatgatcgca ggagcatctt cgatgctgtg 1441 gctcagagta actgccagga gctggagagc ctgctgccct tcctgcagag gagcaagaag 1501 cgcctgactg acagcgagtt caaagaccca gagacaggaa agacctgtct gctaaaagcc 1561 atgctcaatc tgcacaatgg gcagaatgac accatcgctc tgctcctgga cgttgcccgg 1621 aagacagaca gcctgaagca gtttgtcaat gccagctaca cagacagcta ctacaagggc 1681 cagacagcac tgcacattgc cattgaacgg cggaacatga cgctggtgac cctcttggtg 1741 gagaatggag cagatgtcca ggctgcggct aacggggact tcttcaagaa aaccaaaggg 1801 aggcctggct tctactttgg tgagctgccc ctgtccctgg ctgcgtgcac caaccagctg 1861 gccattgtga agttcctgct gcagaactcc tggcagcctg cagacatcag cgcccgggac 1921 tcagtgggca acacggtgct tcatgccctg gtggaggtgg cagataacac agttgacaac 1981 accaagttcg tgacaagcat gtacaacgag atcttgatcc tgggggccaa actccacccc 2041 acgctgaagc tggaagagat caccaacagg aaggggctca cgccactggc tctggctgct 2101 agcagtggga agatcggggt cttggcctac attctccaga gggagatcca tgaacccgag 2161 tgccgacacc tatccaggaa gttcaccgaa tgggcctatg ggccagtgca ctcctccctt 2221 tatgacctgt cctgcattga cacctgtgaa aagaactcgg ttctggaggt gatcgcttac 2281 agcagcagtg agacccctaa ccgtcatgac atgcttctcg tggaaccctt gaaccgactc 2341 ctacaggaca agtgggacag atttgtcaag cgcatcttct acttcaactt cttcgtctac 2401 tgcttgtata tgatcatctt caccgcggct gcctactatc ggcctgtgga aggcttgccc 2461 ccctataagc tgaaaaacac cgttggggac tatttccgag tcaccggaga gatcttgtct 2521 gtgtcaggag gagtctactt cttcttccga gggattcaat atttcctgca gaggcgacca 2581 tccctcaaga gtttgtttgt ggacagctac agtgagatac ttttctttgt acagtcgctg 2641 ttcatgctgg tgtctgtggt actgtacttc agccaacgca aggagtatgt ggcttccatg 2701 gtgttctccc tggccatggg ctggaccaac atgctctact atacccgagg attccagcag 2761 atgggcatct atgctgtcat gattgagaag atgatcctca gagacctgtg ccggtttatg 2821 ttcgtctacc tcgtgttctt gtttggattt tccacagctg tggtgacact gattgaggat 2881 gggaagaata actctctgcc tatggagtcc acaccacaca agtgccgggg gtctgcctgc 2941 aagccaggta actcttacaa cagcctgtat tccacatgtc tggagctgtt caagttcacc 3001 atcggcatgg gcgacctgga gttcactgag aactacgact tcaaggctgt cttcatcatc 3061 ctgttactgg cctatgtgat tctcacctac atccttctgc tcaacatgct cattgctctc 3121 atgggtgaga ccgtcaacaa gattgcacaa gagagcaaga acatctggaa gctgcagaga 3181 gccatcacca tcctggatac agagaagagc ttcctgaagt gcatgaggaa ggccttccgc 3241 tctggcaagc tgctgcaggt ggggttcact cctgacggca aggatgacta ccggtggtgt 3301 ttcagggtgg acgaggtaaa ctggactacc tggaacacca atgtgggtat catcaacgag 3361 gacccaggca actgtgaggg cgtcaagcgc accctgagct tctccctgag gtcaggccga 3421 gtttcaggga gaaactggaa gaactttgcc ctggttcccc ttctgaggga tgcaagcact 3481 cgagatagac atgccaccca gcaggaagaa gttcaactga agcattatac gggatccctt 3541 aagccagagg atgctgaggt tttcaaggat tccatggtcc caggggagaa ataatggaca 3601 ctatgcaggg atcaatgcgg ggtctttggg tggtctgctt agggaaccag cagggttgac 3661 gttatctggg tgatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa 3721 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt 3781 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg 3841 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct 3901 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag 3961 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt 4021 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta 4081 tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga tcataatcag 4141 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 4201 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 4261 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 4321 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg atatctataa 4381 caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt 4441 atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac 4501 gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga 4561 gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa 4621 gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt 4681 aatctagctg catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag 4741 ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc 4801 ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac 4861 gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt 4921 tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag 4981 cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt 5041 gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg 5101 ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg 5161 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 5221 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 5281 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 5341 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 5401 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 5461 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 5521 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 5581 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 5641 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 5701 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 5761 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 5821 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 5881 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 5941 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 6001 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 6061 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 6121 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 6181 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 6241 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 6301 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 6361 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 6421 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 6481 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 6541 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 6601 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 6661 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 6721 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 6781 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 6841 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 6901 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 6961 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 7021 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 7081 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 7141 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 7201 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 7261 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 7321 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat 7381 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 7441 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 7501 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct 7561 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 7621 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 7681 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 7741 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca 7801 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 7861 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 7921 aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg gaggacgggc 7981 agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg ctcgacacgc 8041 tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg tacgttaaag 8101 ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat 8161 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa 8221 caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa aatttcttct 8281 ataaagtaac aaaactttta tcgaattcct gcagcccggg ggatgttccc acaatggtta 8341 attcgagctc gcccggggat ctaattcaat tagagactaa ttcaattaga gctaattcaa 8401 ttaggatcca agcttatcga tttcgaaccc tcgaccgccg gagtataaat agaggcgctt 8461 cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc 8521 taagcaaata aacaagcgca gctgaacaag ctaaacaatc ggggtaccgc tagagtcgac 8581 ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt 8641 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 8701 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 8761 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct 8821 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 8881 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 8941 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 9001 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 9061 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 9121 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 9181 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag 9241 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 9301 gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc 9361 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 9421 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 9481 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 9541 tctagttgtg gtttgtccaa actcatcaat gtatcttaac tagagcggcc gccaccgcgg 9601 tcacgtgtca ctgggtcagt gggtaatcgc ttatcctcgg ataaacaatt atcctcacgg 9661 gtaatcgctt atccgctcgg gtaatcgctt atcctcgggt aatcgcttat ccttgggtaa 9721 tcgcttatcc tcggataaac aattatcctc acgggtaatc gcttatccgc tcgggtaatc 9781 gcttatcctc gggtaatcgc ttatccttgg gtaatcgctt atcctcggat aaacaattat 9841 cctcacgggt aatcgcttat ccgctcgggt aatcgcttat cctcgggtaa tcgcttatcc 9901 tttcacgttg ggactcagtg aggaggacct gaattcctgc agcccgagcg gagactctag 9961 cg //