LOCUS Exported 5576 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION For use in light-induced protein inactivation through the interaction with CIB1. ACCESSION . VERSION . KEYWORDS pCS2-CRY2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5576) AUTHORS Rathbun LI, Colicino EG, Manikas J, O'Connell J, Krishnan N, Reilly NS, Coyne S, Erdemci-Tandogan G, Garrastegui A, Freshour J, Santra P, Manning ML, Amack JD, Hehnly H TITLE Cytokinetic bridge triggers de novo lumen formation in vivo. JOURNAL Nat Commun. 2020 Mar 9;11(1):1269. doi: 10.1038/s41467-020-15002-8. PUBMED 32152267 REFERENCE 2 (bases 1 to 5576) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5576 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..978 /label=CMV IE94 promoter /note="enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94" promoter 1013..1031 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 1013..1030 /label=SP6 /note="SP6 promoter, forward primer" promoter complement(2584..2601) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 2606..2740 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2630..2649) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2684..2703 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(2853..2873) /label=T3 /note="T3 promoter, forward primer" promoter complement(2853..2871) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(2892..2908) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2892..2908) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2905..2927) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 2916..2932 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2940..2970) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2985..3006 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3123..3140) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3294..3882) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3374..3393) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4053..4913) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4676..4695 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4914..5018) /gene="bla" /label=AmpR promoter rep_origin complement(5044..5499) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5181..5202) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 5393..5412 /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt 61 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca 121 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ctggcactgt 181 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg 241 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat 301 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca 361 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt 421 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac 481 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac 541 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta 601 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa 661 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg 721 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac 781 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg 841 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa 901 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc 961 aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt 1021 gacactatag aatacaagct acttgttctt tttgcagcat gaagatggac aaaaagacca 1081 tcgtctggtt tcggagagat ttgagaatag aagataatcc cgcgctcgcc gccgcggccc 1141 acgagggttc cgtcttcccc gttttcattt ggtgtcctga agaagaaggc cagttttatc 1201 ccggaagggc ctctaggtgg tggatgaagc aaagtctggc ccatcttagc cagtcactga 1261 aagcactggg cagtgatctt accctgatca agacacacaa taccatctct gccatcctcg 1321 actgcatcag ggtgaccggc gcaacgaaag tcgtgtttaa ccacctgtac gatccagtta 1381 gtctggtgcg cgaccacact gtgaaggaga agctggtgga acgggggatc agtgtgcaga 1441 gctacaacgg ggaccttctg tacgagccat gggagatcta ttgcgagaaa gggaaaccgt 1501 tcacctcctt caacagttac tggaagaaat gtttggatat gtcaatagag tccgttatgt 1561 tgccccctcc ctggagactg atgccgatta ctgctgctgc agaggccatc tgggcctgct 1621 ccatcgagga actcggtctg gaaaatgaag cagaaaagcc aagcaatgca cttctcacta 1681 gagcctggag ccccggctgg tctaatgccg acaagctgct taacgagttc atcgaaaaac 1741 aactgattga ctacgcgaag aactccaaga aagtggtagg taactcaact agcttgctct 1801 ctccatatct ccattttggc gagatttctg tccgccatgt atttcagtgc gctcggatga 1861 aacagattat ctgggctcgc gataaaaaca gcgaaggcga agaaagcgcc gatctgttcc 1921 tgcgagggat cggacttcgg gaatactccc ggtatatatg tttcaacttt ccattcacac 1981 acgagcagag tctgttgtcc cacctcaggt tcttcccctg ggacgccgat gtcgacaaat 2041 tcaaggcatg gagacaggga aggacagggt acccactcgt ggatgctggc atgagagagc 2101 tctgggctac aggctggatg cacaaccgca tccgggtaat cgtgtcctca tttgctgtca 2161 agtttctgct cctgccttgg aaatggggaa tgaagtactt ttgggatacc cttctcgacg 2221 ccgacttgga gtgtgacatt ctgggatggc aatatattag cgggtcaatt cctgacggcc 2281 atgagttgga caggttggac aatccggcct tgcagggagc taagtatgat cccgaaggag 2341 agtatattcg acagtggctc cccgagctgg cccgacttcc tacggagtgg attcaccatc 2401 cttgggacgc accactgaca gtgctcaagg caagcggggt ggagctgggc accaattacg 2461 ctaagcctat agttgatata gatacagcac gcgagctgct ggctaaagcg atctctcgca 2521 ctcgggaggc gcagattatg atcggtgctg ccggcagcgg cagcccaccg ctgtagtcta 2581 gaactatagt gagtcgtatt acgtagatcc agacatgata agatacattg atgagtttgg 2641 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 2701 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 2761 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taattcgcgg ccgcggcgcc 2821 aatgcattgg gcccggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg 2881 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 2941 aacatacgag ccgggagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 3001 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 3061 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 3121 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 3181 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 3241 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 3301 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 3361 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 3421 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 3481 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 3541 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 3601 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 3661 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 3721 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 3781 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 3841 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 3901 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 3961 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 4021 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 4081 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 4141 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 4201 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 4261 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 4321 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 4381 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 4441 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 4501 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 4561 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 4621 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 4681 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 4741 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 4801 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 4861 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 4921 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 4981 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 5041 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 5101 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 5161 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 5221 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 5281 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 5341 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 5401 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 5461 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc 5521 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag tcgacc //