LOCUS Exported 3434 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Knock Down MQWSH3- Collybistin isoforms. ACCESSION . VERSION . KEYWORDS CBSH3- shRNA1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3434) TITLE de Blas SH3- isoforms of collybistin REFERENCE 2 (bases 1 to 3434) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3434 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..46) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(200..788) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(280..299) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(959..1819) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1582..1601 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1820..1924) /gene="bla" /label=AmpR promoter rep_origin complement(1950..2405) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2087..2108) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2299..2318 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2576..2888 /label=U6 promoter /note="RNA polymerase III promoter for mouse U6 snRNA (Das et al., 1988)" primer_bind 2807..2827 /label=mU6-F /note="Mouse U6 promoter, forward primer" polyA_signal 2961..3095 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2985..3004) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3039..3058 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(3193..3213) /label=T3 /note="T3 promoter, forward primer" promoter complement(3193..3211) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3232..3248) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3232..3248) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3245..3267) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3256..3272 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3280..3310) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3325..3346 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." ORIGIN 1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 1921 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt 1981 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 2041 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 2101 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 2161 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 2221 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 2281 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 2341 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 2401 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 2461 ttcgctatta cgccagtcga cagcctgcag gaatggccgg ccatcttaat taagaaattt 2521 aaatatgttt aaacataggg cccaatggcg cgccaaggcc atctaggcca agcttatccg 2581 acgccgccat ctctaggccc gcgccggccc cctcgcacag acttgtggga gaagctcggc 2641 tactcccctg ccccggttaa tttgcatata atatttccta gtaactatag aggcttaatg 2701 tgcgataaaa gacagataat ctgttctttt taatactagc tacattttac atgataggct 2761 tggatttcta taagagatac aaatactaaa ttattatttt aaaaaacagc acaaaaggaa 2821 actcacccta actgtaaagt aattgtgtgt tttgagacta taaatatccc ttggagaaaa 2881 gccttgtttg ggttcaccca gagcattccc gatcacagat cgggaatgct atgggtgaac 2941 ctttttctag ataatacgta gatccagaca tgataagata cattgatgag tttggacaaa 3001 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt 3061 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta 3121 tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cttgcggccg cggcggtacc 3181 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct 3241 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat 3301 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 3361 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 3421 cgcggggaga ggcg //