LOCUS Exported 3434 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).. ACCESSION . VERSION . KEYWORDS CBSH3- 3m shRNA1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3434) TITLE de Blas SH3- isoforms of collybistin REFERENCE 2 (bases 1 to 3434) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3434 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(529..1389) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1152..1171 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1390..1494) /gene="bla" /label=AmpR promoter rep_origin complement(1520..1975) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1657..1678) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1869..1888 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2146..2458 /label=U6 promoter /note="RNA polymerase III promoter for mouse U6 snRNA (Das et al., 1988)" primer_bind 2377..2397 /label=mU6-F /note="Mouse U6 promoter, forward primer" polyA_signal 2531..2665 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2555..2574) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2609..2628 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(2763..2783) /label=T3 /note="T3 promoter, forward primer" promoter complement(2763..2781) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(2802..2818) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2802..2818) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2815..2837) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 2826..2842 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2850..2880) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2895..2916 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3033..3050) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3204..358) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3284..3303) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 61 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 121 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 181 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 241 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 301 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 361 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 421 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 481 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 541 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 601 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 661 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 721 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 781 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 841 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 901 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 961 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 1021 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 1081 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 1141 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 1201 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 1261 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 1321 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 1381 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 1441 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 1501 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat 1561 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 1621 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 1681 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 1741 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 1801 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 1861 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 1921 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat 1981 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 2041 cgccagtcga cagcctgcag gaatggccgg ccatcttaat taagaaattt aaatatgttt 2101 aaacataggg cccaatggcg cgccaaggcc atctaggcca agcttatccg acgccgccat 2161 ctctaggccc gcgccggccc cctcgcacag acttgtggga gaagctcggc tactcccctg 2221 ccccggttaa tttgcatata atatttccta gtaactatag aggcttaatg tgcgataaaa 2281 gacagataat ctgttctttt taatactagc tacattttac atgataggct tggatttcta 2341 taagagatac aaatactaaa ttattatttt aaaaaacagc acaaaaggaa actcacccta 2401 actgtaaagt aattgtgtgt tttgagacta taaatatccc ttggagaaaa gccttgtttg 2461 ggtacagcct gagcattccc gatcacagat cgggaatgct aaggctgtac ctttttctag 2521 ataatacgta gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag 2581 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac 2641 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt 2701 tcagggggag gtgtgggagg ttttttaatt cttgcggccg cggcggtacc cagcttttgt 2761 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg 2821 tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat aaagtgtaaa 2881 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 2941 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 3001 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 3061 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 3121 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 3181 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 3241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 3301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 3361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 3421 agttcggtgt aggt //