LOCUS       Exported               12678 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  lenti vector encoding the MS2-p300 with a 2A Hygro resistance 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    Lenti_MCP-p300_Hygro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12678)
  AUTHORS   Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson 
            KE, Ni M, Xu J
  TITLE     Interrogation of enhancer function by enhancer-targeting CRISPR 
            epigenetic editing.
  JOURNAL   Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5.
  PUBMED    31980609
REFERENCE   2  (bases 1 to 12678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12678
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1186..1195
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1195..1581
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             1636..1656
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1672..3522
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      3286..3295
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3523..3549
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3568..3621
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3625..4644
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    4684..5272
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4737..4757)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5155..5166)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5275..5291)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5276..5292)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5797..5977
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6003..6020)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6009..6233
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6279..6707
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6366..6385)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6576..6597
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6716..6736)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6721..7050
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6901..7036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6963..6982
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7098..7145
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7164..7538
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7668..7789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7705..7724)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7759..7778
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7838..7854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7838..7854)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7851..7873)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7862..7878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7886..7916)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7931..7952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8069..8086)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8240..8828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8320..8339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8999..9859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9622..9641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9860..9964)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10039..10058)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10230..10609
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10611..10809
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10764..10784
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10827..11007
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11054..11179
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11672..11905
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12090..12134
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12432..12549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatgaagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgac gtacggccac catggcttca
     1201 aactttactc agttcgtgct cgtggacaat ggtgggacag gggatgtgac agtggctcct
     1261 tctaatttcg ctaatggggt ggcagagtgg atcagctcca actcacggag ccaggcctac
     1321 aaggtgacat gcagcgtcag gcagtctagt gcccagaaga gaaagtatac catcaaggtg
     1381 gaggtcccca aagtggctac ccagacagtg ggcggagtcg aactgcctgt cgccgcttgg
     1441 aggtcctacc tgaacatgga gctcactatc ccaattttcg ctaccaattc tgactgtgaa
     1501 ctcatcgtga aggcaatgca ggggctcctc aaagacggta atcctatccc ttccgccatc
     1561 gccgctaact caggtatcta cagcgctgga ggaggtggaa gcggaggagg aggaagcgga
     1621 ggaggaggta gcggacctaa gaaaaagagg aaggtggcgg ccgctggatc cattttcaaa
     1681 ccagaagaac tacgacaggc actgatgcca actttggagg cactttaccg tcaggatcca
     1741 gaatcccttc cctttcgtca acctgtggac cctcagcttt taggaatccc tgattacttt
     1801 gatattgtga agagccccat ggatctttct accattaaga ggaagttaga cactggacag
     1861 tatcaggagc cctggcagta tgtcgatgat atttggctta tgttcaataa tgcctggtta
     1921 tataaccgga aaacatcacg ggtatacaaa tactgctcca agctctctga ggtctttgaa
     1981 caagaaattg acccagtgat gcaaagcctt ggatactgtt gtggcagaaa gttggagttc
     2041 tctccacaga cactgtgttg ctacggcaaa cagttgtgca caatacctcg tgatgccact
     2101 tattacagtt accagaacag gtatcatttc tgtgagaagt gtttcaatga gatccaaggg
     2161 gagagcgttt ctttggggga tgacccttcc cagcctcaaa ctacaataaa taaagaacaa
     2221 ttttccaaga gaaaaaatga cacactggat cctgaactgt ttgttgaatg tacagagtgc
     2281 ggaagaaaga tgcatcagat ctgtgtcctt caccatgaga tcatctggcc tgctggattc
     2341 gtctgtgatg gctgtttaaa gaaaagtgca cgaactagga aagaaaataa gttttctgct
     2401 aaaaggttgc catctaccag acttggcacc tttctagaga atcgtgtgaa tgactttctg
     2461 aggcgacaga atcaccctga gtcaggagag gtcactgtta gagtagttca tgcttctgac
     2521 aaaaccgtgg aagtaaaacc aggcatgaaa gcaaggtttg tggacagtgg agagatggca
     2581 gaatcctttc cataccgaac caaagccctc tttgcctttg aagaaattga tggtgttgac
     2641 ctgtgcttct ttggcatgca tgttcaagag tatggctctg actgccctcc acccaaccag
     2701 aggagagtat acatatctta cctcgatagt gttcatttct tccgtcctaa atgcttgagg
     2761 actgcagtct atcatgaaat cctaattgga tatttagaat atgtcaagaa attaggttac
     2821 acaacagggc atatttgggc atgtccacca agtgagggag atgattatat cttccattgc
     2881 catcctcctg accagaagat acccaagccc aagcgactgc aggaatggta caaaaaaatg
     2941 cttgacaagg ctgtatcaga gcgtattgtc catgactaca aggatatttt taaacaagct
     3001 actgaagata gattaacaag tgcaaaggaa ttgccttatt tcgagggtga tttctggccc
     3061 aatgttctgg aagaaagcat taaggaactg gaacaggagg aagaagagag aaaacgagag
     3121 gaaaacacca gcaatgaaag cacagatgtg accaagggag acagcaaaaa tgctaaaaag
     3181 aagaataata agaaaaccag caaaaataag agcagcctga gtaggggcaa caagaagaaa
     3241 cccgggatgc ccaatgtatc taacgacctc tcacagaaac tatatgccac catggagaag
     3301 cataaagagg tcttctttgt gatccgcctc attgctggcc ctgctgccaa ctccctgcct
     3361 cccattgttg atcctgatcc tctcatcccc tgcgatctga tggatggtcg ggatgcgttt
     3421 ctcacgctgg caagggacaa gcacctggag ttctcttcac tccgaagagc ccagtggtcc
     3481 accatgtgca tgctggtgga gctgcacacg cagagccagg actacccgta cgacgttccg
     3541 gactacgctt cttgtacagg cagtggagag ggcagaggaa gtctgctaac atgcggtgac
     3601 gtcgaggaga atcctggccc aaccatgaaa aagcctgaac tcaccgctac ctctgtcgag
     3661 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc cgagggcgaa
     3721 gaatctcggg ctttcagctt cgatgtggga gggcgtggat atgtcctgcg ggtgaatagc
     3781 tgcgccgatg gtttctacaa agatcgctat gtttatcggc actttgcatc cgccgctctc
     3841 cctattcccg aagtgcttga cattggggag ttcagcgaga gcctgaccta ttgcatctcc
     3901 cgccgtgcac agggtgtcac cttgcaagac ctgcctgaaa ccgaactgcc cgctgttctc
     3961 cagcccgtcg ccgaggccat ggatgccatc gctgccgccg atcttagcca gaccagcggg
     4021 ttcggcccat tcggacctca aggaatcggt caatacacta catggcgcga tttcatctgc
     4081 gctattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcc
     4141 tccgtcgccc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     4201 cacctcgtgc acgccgattt cggctccaac aatgtcctga ccgacaatgg ccgcataaca
     4261 gccgtcattg actggagcga ggccatgttc ggggattccc aatacgaggt cgccaacatc
     4321 ttcttctgga ggccctggtt ggcttgtatg gagcagcaga cccgctactt cgagcggagg
     4381 catcccgagc ttgcaggatc tcctcggctc cgggcttata tgctccgcat tggtcttgac
     4441 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc tcagggtcgc
     4501 tgcgacgcaa tcgtccggtc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     4561 agcgctgccg tctggaccga tggctgtgtg gaagtgctcg ccgatagtgg aaacagacgc
     4621 cccagcactc gtcctagggc aaaggatctg cagtaatgag aattcgatat caagcttatc
     4681 ggtaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4741 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4801 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4861 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4921 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4981 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5041 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     5101 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5161 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5221 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     5281 cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     5341 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     5401 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5461 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     5521 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     5581 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     5641 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     5701 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     5761 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     5821 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5881 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5941 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     6001 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6061 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6121 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6181 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6241 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     6301 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6361 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6421 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6481 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6541 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6601 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6661 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     6721 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6781 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     6841 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6901 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6961 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     7021 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     7081 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     7141 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     7201 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     7261 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     7321 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     7381 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     7441 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     7501 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     7561 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7621 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7681 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7741 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7801 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7861 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7921 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7981 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8041 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8101 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8161 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8221 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8281 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8341 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8401 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8461 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8521 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8581 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8641 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8701 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8761 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8821 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8881 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8941 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9001 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9061 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9961 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
    10021 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
    10081 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
    10141 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
    10201 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
    10261 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
    10321 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
    10381 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
    10441 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
    10501 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
    10561 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
    10621 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
    10681 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
    10741 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
    10801 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
    10861 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    10921 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    10981 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
    11041 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
    11101 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
    11161 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
    11221 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
    11281 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
    11341 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
    11401 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
    11461 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
    11521 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
    11581 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
    11641 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
    11701 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
    11761 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    11821 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    11881 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    11941 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    12001 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    12061 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    12121 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    12181 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    12241 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    12301 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
    12361 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
    12421 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
    12481 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
    12541 caaaattttc gggtttatta cagggacagc agagatccag tttggttaat tagctagctg
    12601 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
    12661 ttgaaaggag tgggaatt
//
