LOCUS Exported 2178 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0391 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2178) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2178) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2178 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 39..56 /label=L4440 /note="L4440 vector, forward primer" terminator 80..137 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." terminator 291..334 /label=bacterial terminator /note="putative bacterial transcription terminator" primer_bind complement(338..356) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" CDS 518..1177 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(584..603) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" terminator 1198..1292 /label=lambda t0 terminator /note="transcription terminator from phage lambda" rep_origin 1475..2063 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1964..1983 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 61 cctgcataac gcgaagtaat cttttcggtt ttaaagaaaa agggcagggt ggtgacacct 121 tgcccttttt tgccggactg cagggtctcg agtacattat atacaaatca gtcactactg 181 ctttcaatga atagcaaaac cttatatagc gcaataatgg ttcgcaataa tggttcgcaa 241 taatggttcc tcactaaaac gacacgcctc cctcctgaga ccgaattcca gaaatcatcc 301 ttagcgaaag ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta 361 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcactcg agttgatcgg 421 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt 481 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata 541 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt 601 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt 661 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa 721 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt 781 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga 841 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg 901 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa 961 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc 1021 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc 1081 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt 1141 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac 1201 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg 1261 gttgccgccg ggcgtttttt attggtgaga atccaagcct cgagctgtca gaccaagttt 1321 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 1381 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 1441 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 1501 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 1561 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 1621 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 1681 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 1741 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 1801 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 1861 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 1921 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 1981 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 2041 caggggggcg gagcctgtgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 2101 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 2161 gtattaccgc ctttgagt //