LOCUS Exported 2224 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0401 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2224) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2224) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2224 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 89..107 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 111..154 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 354..411 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(435..452) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(606..1194) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(686..705) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1377..1471 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1492..2151) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 2066..2085 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" ORIGIN 1 tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac 61 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata 121 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcggtctcag gagggaggcg 181 tgtcgtttta gtgaggtcag cttagcatga gctaagcaca tacgtcagaa accattattg 241 cgcggctatc agctaggtca cgatcactat cagcgcggta ggtcctatat aaggttttgc 301 tattcattga aagcagtagt gactgatttg tatatatact cgagaccctg cagtccggca 361 aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc 421 gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 481 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 541 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 601 gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 661 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 721 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 781 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 841 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 901 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 961 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 1021 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 1081 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 1141 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 1201 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 1261 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 1321 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc 1381 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct 1441 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc 1501 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc 1561 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt 1621 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa 1681 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa 1741 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg 1801 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg 1861 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt 1921 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc 1981 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg 2041 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg 2101 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc 2161 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt 2221 atgg //