LOCUS Exported 902 bp ds-DNA linear SYN 30-MAY-2021 DEFINITION Bacterial expression for structure determination; may not be full ORF. ACCESSION . VERSION . KEYWORDS SGF29 (3MEW) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 902) TITLE Structural Genomics Consortium Clones REFERENCE 2 (bases 1 to 902) AUTHORS . TITLE Direct Submission JOURNAL Exported May 30, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..902 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 35..52 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 65..85 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS complement(524..535) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" CDS 604..621 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(674..692) /label=T7 Term /note="T7 terminator, reverse primer" terminator 688..735 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(859..878) /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg 61 cagagaaaac ttgtatttcc agggcaccct gcccctgtgg atcgggaagc ctggtgacaa 121 gcccccaccc ctctgtgggg ccatccctgc ctcaggagac tacgtggcca gacctggaga 181 caaggtggct gcccgggtga aggccgtgga tggggacgag cagtggatcc tggccgaggt 241 ggtcagttac agccatgcca ccaacaagta tgaggtagat gacatcgatg aagaaggcaa 301 agagagacac accctgagcc ggcgccgtgt catcccgctg ccccagtgga aggccaaccc 361 ggagacggac cctgaggcct tgttccagaa ggagcagctc gtgctggccc tgtatcccca 421 gactacctgc ttctaccgcg ccctgatcca tgcgccccca cagcggcccc aggatgacta 481 ctcggtcctg tttgaagaca cctcctatgc agatggctat tcccctcccc tcaatgtggc 541 tcagagatac gtggtggctt gtaaggaacc caagtgatga cgaagcttgc ggccgcactc 601 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag 661 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc 721 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct 781 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 841 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 901 gc //