LOCUS       Exported                8092 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCXN2-BCL6 (CXN-BCL6)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Vasanwala FH, Kusam S, Toney LM, Dent AL
  TITLE     Repression of AP-1 function: a mechanism for the regulation of 
            Blimp-1 expression and B lymphocyte differentiation by the B cell 
            lymphoma-6 protooncogene.
  JOURNAL   J Immunol. 2002 Aug 15;169(4):1922-9.
  PUBMED    12165517
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          605..1621
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1545..1567
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1629..1648
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3800..3823
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3961..3980)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4026..4081
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4080..4099)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4442..4458)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4442..4458)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4455..4477)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4466..4482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4490..4520)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4535..4556
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4615..4810
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4661..4796
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4723..4742
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    4816..4950
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4853..4872)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4907..4926
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5018..5035)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5189..5777)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5269..5288)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5948..6808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6571..6590
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6809..6913)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7225..8028
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7288..7307)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7898..7917
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8035..8083
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      541 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      601 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      661 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      721 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      781 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      841 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      901 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
      961 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1021 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1081 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1141 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1201 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1261 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1321 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1381 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1441 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1501 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1561 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1621 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcctcga
     1681 ccccaccatg gctagtccgg ctgacagctg tatccagttc acccgccatg ccagtgatgt
     1741 tcttctcaac cttaatcgtc tccggagtcg agacatcttg actgatgttg tcattgttgt
     1801 gagccgtgag cagtttagag cccataaaac ggtcctcatg gcctgcagtg gcctgttcta
     1861 tagcatcttt acagaccagt tgaaatgcaa ccttagtgtg atcaatctag atcctgagat
     1921 caaccctgag ggattctgca tcctcctgga cttcatgtac acatctcggc tcaatttgcg
     1981 ggagggcaac atcatggctg tgatggccac ggctatgtac ctgcagatgg agcatgttgt
     2041 ggacacttgc cggaagttta ttaaggccag tgaagcagag atggtttctg ccatcaagcc
     2101 tcctcgtgaa gagttcctca acagccggat gctgatgccc caagacatca tggcctatcg
     2161 gggtcgtgag gtggtggaga acaacctgcc actgaggagc gcccctgggt gtgagagcag
     2221 agcctttgcc cccagcctgt acagtggcct gtccacaccg ccagcctctt attccatgta
     2281 cagccacctc cctgtcagca gcctcctctt ctccgatgag gagtttcggg atgtccggat
     2341 gcctgtggcc aaccccttcc ccaaggagcg ggcactccca tgtgatagtg ccaggccagt
     2401 ccctggtgag tacagccggc cgactttgga ggtgtccccc aatgtgtgcc acagcaatat
     2461 ctattcaccc aaggaaacaa tcccagaaga ggcacgaagt gatatgcact acagtgtggc
     2521 tgagggcctc aaacctgctg ccccctcagc ccgaaatgcc ccctacttcc cttgtgacaa
     2581 ggccagcaaa gaagaagaga gaccctcctc ggaagatgag attgccctgc atttcgagcc
     2641 ccccaatgca cccctgaacc ggaagggtct ggttagtcca cagagccccc agaaatctga
     2701 ctgccagccc aactcgccca cagagtcctg cagcagtaag aatgcctgca tcctccaggc
     2761 ttctggctcc cctccagcca agagccccac tgaccccaaa gcctgcaact ggaagaaata
     2821 caagttcatc gtgctcaaca gcctcaacca gaatgccaaa ccagaggggc ctgagcaggc
     2881 tgagctgggc cgcctttccc cacgagccta cacggcccca cctgcctgcc agccacccat
     2941 ggagcctgag aaccttgacc tccagtcccc aaccaagctg agtgccagcg gggaggactc
     3001 caccatccca caagccagcc ggctcaataa catcgttaac aggtccatga cgggctctcc
     3061 ccgcagcagc agcgagagcc actcaccact ctacatgcac cccccgaagt gcacgtcctg
     3121 cggctctcag tccccacagc atgcagagat gtgcctccac accgctggcc ccacgttccc
     3181 tgaggagatg ggagagaccc agtctgagta ctcagattct agctgtgaga acggggcctt
     3241 cttctgcaat gagtgtgact gccgcttctc tgaggaggcc tcactcaaga ggcacacgct
     3301 gcagacccac agtgacaaac cctacaagtg tgaccgctgc caggcctcct tccgctacaa
     3361 gggcaacctc gccagccaca agaccgtcca taccggtgag aaaccctatc gttgcaacat
     3421 ctgtggggcc cagttcaacc ggccagccaa cctgaaaacc cacactcgaa ttcactctgg
     3481 agagaagccc tacaaatgcg aaacctgcgg agccagattt gtacaggtgg cccacctccg
     3541 tgcccatgtg cttatccaca ctggtgagaa gccctatccc tgtgaaatct gtggcacccg
     3601 tttccggcac cttcagactc tgaagagcca cctgcgaatc cacacaggag agaaacctta
     3661 ccattgtgag aagtgtaacc tgcatttccg tcacaaaagc cagctgcgac ttcacttgcg
     3721 ccagaagcat ggcgccatca ccaacaccaa ggtgcaatac cgcgtgtcag ccactgacct
     3781 gcctccggag ctcggtaccg actacaaaga cgatgacgac aagtgaggat cccccgggct
     3841 gcaggaattc gatatcaagc ttatcgatac cgtcgaggaa ttcactcctc aggtgcaggc
     3901 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     3961 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     4021 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     4081 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     4141 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     4201 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     4261 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     4321 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     4381 agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat
     4441 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4501 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct
     4621 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4681 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4741 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4801 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4861 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4921 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
     4981 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5041 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5101 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5161 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5221 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5281 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5341 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5401 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5461 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5521 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5581 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5641 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5701 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5761 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5821 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5881 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5941 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6001 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6061 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6121 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6181 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6241 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6301 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6361 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6421 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6481 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6541 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6601 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6661 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6721 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6781 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6841 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6901 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgagcagt gtggttttca
     6961 agaggaagca aaaagcctct ccacccaggc ctggaatgtt tccacccaat gtcgagcagt
     7021 gtggttttgc aagaggaagc aaaaagcctc tccacccagg cctggaatgt ttccacccaa
     7081 tgtcgagcaa accccgccca gcgtcttgtc attggcgaat taattcgaac acgcagatgc
     7141 agtcggggcg gcgcggtccc aggtccactt cgcatattaa ggtgacgcgt gtggcctcga
     7201 acaccgagcg accctgcagc caatatggga tcggccattg aacaagatgg attgcacgca
     7261 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     7321 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     7381 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     7441 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     7501 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     7561 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     7621 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     7681 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     7741 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt gacccatggc
     7801 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     7861 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     7921 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     7981 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagg ggatcggcaa
     8041 taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcggatccg tc
//
