LOCUS Exported 4599 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCI-ASC-HA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4599) AUTHORS Hornung V, Ablasser A, Charrel-Dennis M, Bauernfeind F, Horvath G, Caffrey DR, Latz E, Fitzgerald KA TITLE AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC. JOURNAL Nature. 2009 Mar 26. 458(7237):514-8. PUBMED 19158675 REFERENCE 2 (bases 1 to 4599) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4599 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 581..605 /label=LNCX /note="Human CMV promoter, forward primer" intron 720..852 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 835..854 /label=pMT2-F /note="Synthetic intron, forward primer" promoter 897..915 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 941..967 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" polyA_signal 1576..1697 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1587..1606) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1641..1660 /label=SV40pA-R /note="SV40 polyA, reverse primer" rep_origin 1878..2333 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1965..1984) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2175..2196 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2400..2419) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2519..2541 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(2579..2597) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2665..2769 /gene="bla" /label=AmpR promoter CDS 2770..3630 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2988..3007) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3801..4389 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4290..4309 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc 601 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg 661 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg 721 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac 781 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct 841 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat 901 acgactcact ataggctagc ctcgagaatt cgaaatggcc tacccatacg acgtgccaga 961 ctacgcaggg cgcgcgcgcg acgccatcct ggatgcgctg gagaacctga ccgccgagga 1021 gctcaagaag ttcaagctga agctgctgtc ggtgccgctg cgcgagggct acgggcgcat 1081 cccgcggggc gcgctgctgt ccatggacgc cttggacctc accgacaagc tggtcagctt 1141 ctacctggag acctacggcg ccgagctcac cgctaacgtg ctgcgcgaca tgggcctgca 1201 ggagatggcc gggcagctgc aggcggccac gcaccagggc tctggagccg cgccagctgg 1261 gatccaggcc cctcctcagt cggcagccaa gccaggcctg cactttatag accagcaccg 1321 ggctgcgctt atcgcgaggg tcacaaacgt tgagtggctg ctggatgctc tgtacgggaa 1381 ggtcctgacg gatgagcagt accaggcagt gcgggccgag cccaccaacc caagcaagat 1441 gcggaagctc ttcagtttca caccagcctg gaactggacc tgcaaggact tgctcctcca 1501 ggccctaagg gagtcccagt cctacctggt ggaggacctg gagcggagct aggcggccgc 1561 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt 1621 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa 1681 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg 1741 agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg 1801 atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 1861 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 1921 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 1981 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 2041 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 2101 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 2161 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 2221 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 2281 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg 2341 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt 2401 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac 2461 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc 2521 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc 2581 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca 2641 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 2701 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 2761 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 2821 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 2881 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 2941 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 3001 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 3061 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 3121 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 3181 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 3241 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 3301 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 3361 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 3421 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 3481 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 3541 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 3601 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 3661 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 3721 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 3781 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 3841 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 3901 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 3961 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 4021 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 4081 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 4141 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 4201 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 4261 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 4321 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 4381 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 4441 gctcacatgg ctcgacagat cttcaatatt ggccattagc catattattc attggttata 4501 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt 4561 acatttatat tggctcatgt ccaatatgac cgccatgtt //