LOCUS Exported 6546 bp ds-DNA circular SYN 16-JUN-2022 DEFINITION Expresses myc tagged Miro1. ACCESSION . VERSION . KEYWORDS pRK5-myc-Miro1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6546) AUTHORS Fransson A, Ruusala A, Aspenstrom P TITLE Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis. JOURNAL J Biol Chem. 2003 Feb 21;278(8):6495-502. Epub 2002 Dec 12. PUBMED 12482879 REFERENCE 2 (bases 1 to 6546) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 16, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6546 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 581..605 /label=LNCX /note="Human CMV promoter, forward primer" promoter 815..833 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 815..832 /label=SP6 /note="SP6 promoter, forward primer" primer_bind 846..865 /label=pMT2-F /note="Synthetic intron, forward primer" regulatory 912..921 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 921..950 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" regulatory 2833..2842 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 2846..2980 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2883..2902) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2937..2956 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" promoter 3049..3406 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3072..3092) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3257..3392 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3319..3338 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(3426..3443) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3426..3442) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3435..3457) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3655..4110 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3742..3761) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3952..3973 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4127..4146) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4246..4268 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4306..4324) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4392..4496 /gene="bla" /label=AmpR promoter CDS 4497..5357 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4715..4734) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5528..6116 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6017..6036 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6270..6287 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6404..6425 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6440..6470 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6478..6494 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6483..6505 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6502..6518 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6502..6518 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc 601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat 661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg 721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca 781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat 841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat 901 cgattgaatt ggccaccatg gagcagaagc tgatctccga ggaggacctg ggatctatga 961 agaaagacgt gcggatcctg ctggtgggag aacctagagt tgggaagaca tcactgatta 1021 tgtctctggt cagtgaagaa tttccagaag aggttcctcc ccgggcagaa gaaatcacca 1081 ttccagctga tgtcacccca gagagagttc caacacacat tgtagattac tcagaagcag 1141 aacagagtga tgaacaactt catcaagaaa tatctcaggc taatgtcatc tgtatagtgt 1201 atgccgttaa caacaagcat tctattgata aggtaacaag tcgatggatt cctctcataa 1261 atgaaagaac agacaaagac agcaggctgc ctttaatatt ggttgggaac aaatctgatc 1321 tggtggaata tagtagtatg gagaccatcc ttcctattat gaaccagtat acagaaatag 1381 aaacctgtgt ggagtgttca gcgaaaaacc tgaagaacat atcagagctc ttttattacg 1441 cacagaaagc tgttcttcat cctacagggc ccctgtactg cccagaggag aaggagatga 1501 aaccagcttg tataaaagcc cttactcgta tatttaaaat atctgatcaa gataatgatg 1561 gtactctcaa tgatgctgaa ctcaacttct ttcagaggat ttgtttcaac actccattag 1621 ctcctcaagc tctggaggat gtcaagaatg tagtcagaaa acatataagt gatggtgtgg 1681 ctgacagtgg gttgaccctg aaaggttttc tctttttaca cacacttttt atccagagag 1741 ggagacacga aactacttgg actgtgcttc gacgatttgg ttatgatgat gacctggatt 1801 tgacacctga atatttgttc cccctgctga aaatacctcc tgattgcact actgaattaa 1861 atcatcatgc atatttattt ctccaaagca cctttgacaa gcatgatttg gatagagact 1921 gtgctttgtc acctgatgag cttaaagatt tatttaaagt tttcccttac ataccttggg 1981 ggccagatgt gaataacaca gtttgtacca atgaaagagg ctggataacc taccagggat 2041 tcctttccca gtggacgctc acgacttatt tagatgtaca gcggtgcctg gaatatttgg 2101 gctatctagg ctattcaata ttgactgagc aagagtctca agcttcagct gttacagtga 2161 caagagataa aaagatagac ctgcagaaaa aacaaactca aagaaatgtg ttcagatgta 2221 atgtaattgg agtgaaaaac tgtgggaaaa gtggagttct tcaggctctt cttggaagaa 2281 acttaatgag gcagaagaaa attcgtgaag atcataaatc ctactatgcg attaacactg 2341 tttatgtata tggacaagag aaatacttgt tgttgcatga tatctcagaa tcggaatttc 2401 taactgaagc tgaaatcatt tgtgatgttg tatgcctggt atatgatgtc agcaatccca 2461 aatcctttga atactgtgcc aggattttta agcaacactt tatggacagc agaatacctt 2521 gcttaatcgt agctgcaaag tcagacctgc atgaagttaa acaagaatac agtatttcac 2581 ctactgattt ctgcaggaaa cacaaaatgc ctccaccaca agccttcact tgcaatactg 2641 ctgatgcccc cagtaaggat atctttgtta aattgacaac aatggccatg tatccgcacg 2701 tgacacaagc tgacctcaag agctccacgt tttggcttcg agcaagtttt ggtgctactg 2761 tttttgcagt tttgggcttt gctatgtaca aagcattatt gaaacagcga tgagaattcc 2821 tgcagaagct tggccgccat ggcccaactt gtttattgca gcttataatg gttacaaata 2881 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 2941 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gggaattaat tcggcgcagc 3001 accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa 3061 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 3121 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 3181 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 3241 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 3301 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 3361 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tgttaacagc 3421 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 3481 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 3541 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt 3601 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc 3661 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 3721 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 3781 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 3841 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc 3901 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 3961 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga 4021 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 4081 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg 4141 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 4201 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 4261 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 4321 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 4381 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 4441 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 4501 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 4561 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 4621 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 4681 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 4741 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 4801 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 4861 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 4921 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 4981 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 5041 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 5101 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 5161 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 5221 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 5281 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 5341 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 5401 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 5461 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 5521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 5581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 5641 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 5701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 5761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 5821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 5881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 5941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 6001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 6061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 6121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 6181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 6241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 6301 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 6361 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 6421 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 6481 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat taattcgagc 6541 tcgccc //