LOCUS Exported 12197 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses Tet1 in mammalian cells. ACCESSION . VERSION . KEYWORDS pcDNA3-Tet1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12197) AUTHORS Wang Y, Zhang Y TITLE Regulation of TET protein stability by calpains. JOURNAL Cell Rep. 2014 Jan 30;6(2):278-84. doi: 10.1016/j.celrep.2013.12.031. Epub 2014 Jan 9. PUBMED 24412366 REFERENCE 2 (bases 1 to 12197) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12197 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" intron 684..1256 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" CDS 1302..1325 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" promoter complement(7516..7534) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(7517..7534) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(7554..7571) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7560..7784 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7830..8258 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7917..7936) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8127..8148 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8267..8287) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8272..8601 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8452..8587 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8514..8533 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 8668..9462 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(8722..8741) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 9332..9351 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 9636..9757 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9673..9692) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9727..9746 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9806..9822) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9806..9822) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9819..9841) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9830..9846 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9854..9884) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9899..9920 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10037..10054) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10208..10796) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10288..10307) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10967..11827) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11590..11609 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11828..11932) /gene="bla" /label=AmpR promoter primer_bind complement(12007..12026) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagcta 661 gagtcgatcc tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg 721 ctattgtaaa attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg 781 gaagatgtcc cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac 841 tctgttgaca accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa 901 actttagctt gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta 961 agtactttct ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag 1021 cacagtttta gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa 1081 attctggctg gcgtggaaat attcttattg gtagaaacaa ctacatcctg gtcatcatcc 1141 tgcctttctc tttatggtta caatgatata cactgtttga gatgaggata aaatactctg 1201 agtccaaacc gggcccctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc 1261 tgggcaacgt gctggtttgt gctgtcgacc ccaagcttat ggactacaaa gacgatgacg 1321 acaagggatc ctctcggtcc cgccccgcaa agccttccaa atcagtcaaa acaaagctac 1381 agaaaaagaa agacatccag atgaagacga agacatcaaa gcaggctgtc agacatgggg 1441 catcagcgaa ggcagtgaac cccggaaaac cgaagcaatt aattaaaaga agagatggta 1501 agaaggaaac ggaagacaaa accccgacac cggcgccgag ttttctgacg cgagccgggg 1561 ccgcccgaat gaatcgggat cgaaaccaag ttctttttca gaacccagac tccttaactt 1621 gcaacgggtt tacaatggct cttcgaagaa cctctcttag ctggcgactc tcccagcgcc 1681 cagtagtcac acccaaacct aagaaagtac caccttcaaa gaagcagtgt acacataata 1741 tccaggacga gccgggggta aagcactctg aaaatgattc agtccccagc caacacgcta 1801 ccgtgtcccc aggcacagag aatggtgaac agaacaggtg tttagttgaa ggggagagcc 1861 aagagataac ccagtcttgc cctgtatttg aggaaagaat agaagatacc caaagctgta 1921 tttccgcatc tgggaacctg gaagcagaaa tttcttggcc actggaaggg acacattgtg 1981 aagaactact ttctcatcag acatctgata atgagtgcac ttcccctcaa gagtgtgctc 2041 cattgcccca aagatcaact tccgaagtta cctctcagaa aaacactagc aatcagttag 2101 cagacttgag ctcacaagta gagtctatca agctgtctga tccttctccg aatcctacgg 2161 gaagcgacca taatggcttt ccagactcca gttttaggat cgttcctgaa ttggacctga 2221 aaacctgcat gcctcttgat gagtctgtgt atcccacagc tctgataaga ttcatcttgg 2281 caggctcaca gccggatgtc tttgatacta agccccaaga aaaaaccctt ataacaaccc 2341 cagaacaagt gggaagccac ccaaatcaag ttctggatgc cacctctgtc ctaggacagg 2401 cctttagtac tcttccactt cagtggggat tttctggtgc taacttagtt caagttgagg 2461 ccctgggcaa gggttcagac tcaccagagg atcttggtgc tattactatg ctaaaccaac 2521 aagaaactgt ggctatggat atggatagga atgctacccc ggacctgcct atcttccttc 2581 ctaagcctcc aaacacagtt gctacctaca gtagccctct tttagggcct gagccccaca 2641 gctctacctc ctgtgggctt gaagtccaag gtgctacccc gattttaact ttggactcag 2701 gacacactcc ccaactccca ccaaaccctg agtcaagttc agtacctctg gtaatagctg 2761 caaacggtac gcgagctgag aagcagtttg gtaccagcct tttcccagcc gtcccacaag 2821 ggtttacagt cgcagccgag aatgaagtcc agcatgcccc attagatctt acccagggtt 2881 ctcaggctgc tcccagcaaa ctggaaggag agatttctcg ggtcagcatc actggctcag 2941 ctgatgtcaa agccacagcg atgtccatgc cggttacaca ggccagtacc tcttctcccc 3001 catgtaattc tacgccgcca atggtggaga ggaggaagcg caaggcctgt ggggtctgtg 3061 agccgtgcca gcagaaggcc aactgtgggg agtgcaccta ctgcaagaat cgaaagaaca 3121 gccaccagat ctgtaagaag aggaaatgcg aggtgctcaa aaagaagcca gaggccacct 3181 cacaggcaca ggttacaaaa gaaaacaaga ggccccagag ggaaaagaag cccaaagttt 3241 taaagacaga ctttaacaac aaaccagtaa atggccccaa atcagagtcc atggactgca 3301 gtagacgtgg tcatggggaa gaagagcaaa ggttggattt gatcacacat ccccttgaaa 3361 atgtaaggaa gaatgcaggc ggcatgacgg gaattgaggt ggagaagtgg gcgccaaaca 3421 agaaatcaca cttagcagag ggtcaagtca aaggaagctg tgatgccaat ttaactgggg 3481 tggaaaaccc ccagccctct gaagatgaca agcagcaaac caacccttct ccgacatttg 3541 cccagaccat aaggaacggc atgaaaaatg tacactgttt acccactgac acacaccttc 3601 cgctcaacaa attgaatcat gaggaattta gcaaggcgtt gggaaataat tcctctaaat 3661 tgctgactga tccctcaaac tgcaaggatg ccatgagtgt caccacctcc gggggggagt 3721 gtgatcatct caaggggcca aggaacaccc tgctcttcca gaagcctggc ttgaactgca 3781 ggtctggtgc agaacccacc atctttaata atcaccctaa tacacacagc gctgggagtc 3841 gacctcaccc tcctgagaag gtacccaaca aagaacccaa agatggctct ccggttcagc 3901 caagtcttct atccctaatg aaggacagga gattaacgct ggaacaagtg gtagccatag 3961 aagccctgac tcagctctca gaagcgccct cagagagctc ctccccatcc aagccggaga 4021 aggatgaaga agcgcatcag aaaacggcta gtctgcttaa cagttgcaag gctatccttc 4081 attcggtgag aaaagacctc caagacccaa acgttcaagg aaagggcctt caccatgaca 4141 ctgttgtctt taatggccag aacagaacat tcaagtcacc tgatagtttc gctactaacc 4201 aagcacttat aaaatcacaa ggatatccaa gttcacctac agctgagaag aaaggcgctg 4261 ctggtgggag agctcccttt gatggttttg agaattcaca tccattgccc atagaaagtc 4321 acaatcttga aaactgcagt caggtactaa gctgcgatca gaatttgagt tcccatgacc 4381 catcatgcca ggatgcaccc tacagtcaaa ttgaagaaga tgtcgcagca caattgaccc 4441 aacttgcatc cacgattaat cacatcaacg ccgaggtcag gaatgctgaa agtacaccag 4501 aaagccttgt tgcaaagaac acaaagcaaa aacacagtca ggagaaacgc atggtacatc 4561 agaagccacc ctctagtaca caaactaaac ccagtgtacc atcagcaaag ccaaagaagg 4621 cccagaaaaa ggcaagagcg accccacacg caaacaagcg gaaaaagaaa cccccagccc 4681 gaagctctca agaaaatgat cagaagaagc aagagcagct agctatagag tatagtaaaa 4741 tgcatgacat ttggatgtca tctaagtttc aaaggtttgg ccagtctagt ccacgtagtt 4801 ttcctgttct gttgagaaat attcctgtct ttaaccaaat attaaagccc gtgactcaga 4861 gtaaaacacc ctcacaacat aatgagttat ttcctcctat caatcagata aaattcacaa 4921 gaaaccctga attggcaaag gaaaaagtga aggttgaacc atcagattct ctgccaacat 4981 gccaattcaa gacagaatcc ggtgggcaga catttgcaga gccggcagat aattctcagg 5041 gacagcccat ggtgagtgtc aatcaggaag ctcaccctct gccccagtcc ccaccatcta 5101 accagtgtgc taatataatg gctggtgctg cccaaacaca gtttcatcta ggcgctcaag 5161 agaacctggt gcatcagata ccaccaccga cgttgcctgg tacctcccct gacacactct 5221 tgccagaccc agcatcaatc ctcaggaaag ggaaggtgtt acatttcgat ggaattacag 5281 ttgttacgga gaagcgtgaa gctcaaacat caagcaatgg accactgggt cccaccacgg 5341 acagtgcaca gtcagaattt aaagagagca tcatggactt acttagcaag cctgcaaaaa 5401 acctgatagc tggattgaag gaacaggaag ctgcaccctg tgactgtgat ggaggtacac 5461 aaaaagaaaa aggcccatat tatacacacc ttggggcagg accaagtgtg gctgctgtca 5521 gggagctcat ggagactagg tttggccaga aggggaaggc aatccggatt gagaagatag 5581 tgttcacggg gaaggaaggg aagagctctc agggctgccc ggtcgccaag tgggtgatca 5641 gaagaagtgg tcctgaagag aagcttattt gtttggttcg tgagcgtgta gaccatcact 5701 gttcgacggc tgtgatagtt gtccttatcc tgctgtggga aggtatccct cgcctgatgg 5761 ctgaccgcct gtacaaagag ctcactgaga acttgaggtc ctacagcgga catcccacag 5821 accgaagatg taccctcaac aaaaagcgta cctgcacctg tcaaggcatc gacccaaaaa 5881 cctgcggagc gtccttctcc tttggctgtt cgtggagcat gtatttcaac ggctgtaagt 5941 ttgggaggag tgaaaacccc agaaaattca gacttgctcc aaactacccc ttacataact 6001 actataagag aattactgga atgagttctg aaggaagtga cgtgaaaacc gggtggatca 6061 ttccagaccg caagaccctc ataagcagag aggaaaaaca gcttgaaaag aatttacaag 6121 aattggctac agtattagct ccactttaca agcagatggc tccagttgct tatcaaaatc 6181 aggtggaata tgaagaagtt gctggagact gtcgacttgg aaatgaagag gggcgtcctt 6241 tctctggtgt cacctgttgc atggattttt gtgcccattc tcacaaggac attcacaaca 6301 tgcacaacgg aagcaccgtg gtgtgtacgt tgattcgagc agatggccgt gacacaaatt 6361 gtcccgagga tgaacaactc cacgtcctgc cactataccg gcttgcagac actgatgaat 6421 ttggctccgt ggaagggatg aaggccaaaa tcaaatctgg ggccatccaa gtcaatgggc 6481 caaccaggaa gaggcgacta cgttttactg agcctgttcc tcgatgtggg aagagggcca 6541 aaatgaagca gaaccacaat aaatcaggtt cacacaacac taagagcttt tcatcagcct 6601 catctacttc tcacctagtg aaagacgaat ctacagactt ctgtcccctg caggcttcct 6661 ccgcagaaac atctacctgt acgtacagta aaacagcctc aggtgggttt gcagaaacaa 6721 gtagtattct ccactgcaca atgccttctg gagcacacag tggtgctaat gcagctgctg 6781 gggaatgtac tggaacggtg cagcctgccg aggtggctgc tcatcctcac cagtctcttc 6841 ccacagccga ttctcccgtt catgctgagc ctctcactag tccatctgag cagctaactt 6901 ctaaccagtc aaaccagcag ctccctctcc tcagcaattc tcagaaactg gcttcctgtc 6961 aggtggaaga tgagcggcac cctgaagcgg atgagcctca gcaccccgag gacgataact 7021 tgcctcaact tgatgaattc tggtcagaca gtgaggagat ctacgccgat ccttcctttg 7081 gtggcgtggc gatagcaccc attcacggct cggtgctcat tgagtgcgct cggaaggagc 7141 ttcatgctac cacctctttg cgctccccca aacgaggggt cccttttcgt gtgtcccttg 7201 tattctacca gcacaaaagc ctaaacaagc ctaatcatgg ttttgatatc aacaaaatta 7261 agtgtaaatg caaaaaagta acgaaaaaaa agcccgcaga ccgggagtgt cctgatgtat 7321 cccccgaagc caatttatca caccaaattc cttctcgagt tgcatcaacc ttaacccgag 7381 acaatgttgt taccgtgtcc ccatactctc tcactcatgt tgcgggaccc tacaatcgtt 7441 gggtctaaaa agggcgaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca 7501 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac 7561 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 7621 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 7681 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 7741 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag 7801 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc 7861 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 7921 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 7981 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 8041 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 8101 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 8161 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 8221 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag 8281 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 8341 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 8401 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 8461 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 8521 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt 8581 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat 8641 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct 8701 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc 8761 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc 8821 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc 8881 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg 8941 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag 9001 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc 9061 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt 9121 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc 9181 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc 9241 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg 9301 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag 9361 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg 9421 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg 9481 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct 9541 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc 9601 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg 9661 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 9721 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct 9781 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 9841 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 9901 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 9961 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 10021 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 10081 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 10141 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 10201 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 10261 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 10321 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 10381 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 10441 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 10501 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 10561 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 10621 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 10681 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 10741 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 10801 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 10861 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 10921 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 10981 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 11041 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 11101 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 11161 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 11221 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 11281 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 11341 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 11401 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 11461 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 11521 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 11581 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 11641 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 11701 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 11761 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 11821 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 11881 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 11941 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac 12001 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt 12061 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac 12121 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg 12181 ggccagatat acgcgtt //