LOCUS       Exported                9686 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    GPR64-Tango
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4010..5011
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(4077..4096)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(5043..5060)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5049..5273
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5319..5747
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5406..5425)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5616..5637
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5756..5776)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5761..6090
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5941..6076
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6003..6022
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6157..6951
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6211..6230)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6821..6840
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7125..7246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7162..7181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7216..7235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7295..7311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7295..7311)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7308..7330)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7319..7335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7343..7373)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7388..7409
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7526..7543)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7697..8285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7777..7796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8456..9316)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9079..9098
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9317..9421)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9496..9515)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttctgcagat atccagcaca gtggcggccg cgccaccatg aagacgatca tcgccctgag
      781 ctacatcttc tgcctggtat tcgccgacta caaggacgat gatgacgcca gcatcgatat
      841 ggtctttagc gtgaggcagt gcggccatgt tggaagaaca gaagaggtgc ttcttacttt
      901 taagattttt ctggttatca tctgtctgca cgtagtactt gtcacaagtc tggaagagga
      961 tacagacaac agttcactca gcccgcctcc ggctaaactg tccgtggttt ctttcgcccc
     1021 gtccagtaac ggtacaccag aggttgagac tacaagtctg aatgacgtca cactgtcact
     1081 tttgcccagc aatgaaacag agaagaccaa gatcaccatt gtgaagacct tcaatgcgtc
     1141 cggagtcaaa ccacagagga atatctgtaa tctgtcctcc atatgtaatg actccgcctt
     1201 ctttagggga gaaatcatgt tccagtatga caaagaatcc acagtgccac aaaaccagca
     1261 catcaccaat gggacactga caggagtgct ctcccttagt gagctgaagc gctcagagct
     1321 gaacaagact ctgcagactc tgtccgaaac atactttatc atgtgtgcca ccgctgaggc
     1381 gcaaagcacc ctcaactgca ccttcacaat aaagttgaac aacaccatga atgcgtgcgc
     1441 agtaatagcc gcccttgaga gggttaaaat caggcctatg gagcactgct gctgctctgt
     1501 ccgaattcca tgcccatcct caccagaaga gctggaaaag ctgcagtgtg acttgcagga
     1561 tccgattgtt tgtcttgccg atcacccacg gggacctcct ttttccagtt cccagtccat
     1621 accagttgtt cccagagcca ctgtcctgtc acaggtgcct aaggctactt ctttcgctga
     1681 acctcctgac tactccccag ttactcacaa tgttccctcc cccatcgggg agatacagcc
     1741 cctgagtccc caaccatctg ctcccattgc atccagcccc gctattgaca tgccacccca
     1801 atctgaaacc atctcaagtc ccatgccgca gactcacgtg tccggaacac caccgccagt
     1861 caaggcgtcc tttagttctc cgacagttag cgcccctgca aatgtgaaca caacttctgc
     1921 accacctgtt caaacagata tcgtgaatac ctcctcaatt agcgaccttg agaatcaggt
     1981 gcttcagatg gagaaggctc tgtctctggg ctcattggag cctaacctgg caggcgaaat
     2041 gattaatcag gttagcaggc tgttgcattc tcctcccgat atgctcgctc cactggctca
     2101 acgcctcctg aaagtggttg atgatatcgg cctgcagctc aacttctcta acactacgat
     2161 tagcctcaca agcccttccc tggccctggc ggtgatcagg gtcaacgctt catctttcaa
     2221 cacaacaacc tttgtggcac aagaccccgc taatctgcag gtcagcctgg agactcaagc
     2281 accggaaaat tccataggga ccattacctt gccctcttcc ctgatgaaca atttgccggc
     2341 tcatgacatg gaacttgcta gtcgcgtgca gtttaacttt tttgaaaccc cggcgctgtt
     2401 ccaggatcct agcctggaga atctgtccct gattagctat gtcatctcct catcagtcgc
     2461 aaatctgacc gtaaggaacc tcactcgaaa cgtgaccgtc acactgaagc acatcaatcc
     2521 tagtcaggat gagttgactg tgcggtgcgt gttttgggat ctgggacgaa atggaggccg
     2581 gggcggctgg tccgacaacg gttgctccgt aaaggaccga agactgaatg agacaatttg
     2641 tacctgcagc catctgacga gctttggcgt tttgctggat ctttcccgga ccagcgtgct
     2701 tcccgcacaa atgatggcat tgaccttcat cacctatatc ggttgcggac tgagttccat
     2761 ctttttgagc gtgacgctgg tcacgtacat tgcgttcgag aagattagga gagattaccc
     2821 ttctaagatc ttgatccagc tctgtgccgc gctccttttg ctcaacctgg tgtttctgct
     2881 cgactcctgg attgccctgt acaaaatgca gggactgtgt attagcgtag ctgttttcct
     2941 gcactacttt cttcttgtgt cttttacgtg gatgggtctg gaagcctttc acatgtacct
     3001 cgccctggtg aaagtgttta acacgtacat ccgcaagtat attctcaagt tctgcatcgt
     3061 cgggtggggt gtgcctgcag tcgtcgtgac catcattctg acaatcagcc ccgacaacta
     3121 tggcctggga agctatggca agtttccaaa cggatcaccc gatgacttct gttggatcaa
     3181 taacaacgct gtgttctata ttaccgtggt gggatatttc tgcgtcattt tcctgctgaa
     3241 cgtcagtatg ttcatcgttg tgctggtgca actgtgcaga atcaagaaaa aaaagcaact
     3301 gggagcacag aggaaaacat ccatccagga tctgcggtct atcgctggct tgacttttct
     3361 gctggggatt acctgggggt tcgctttttt cgcctggggc cccgtgaacg ttactttcat
     3421 gtatctgttt gccatcttta ataccctgca gggcttcttt atttttattt tttattgtgt
     3481 tgctaaggag aatgtgcgga aacagtggag aagatacctg tgttgcggga agttgcgact
     3541 tgccgagaac agtgactgga gcaagaccgc aactaacggg ctcaagaagc aaacggtcaa
     3601 tcagggtgtg agctcatcct ctaatagcct gcagtcctca tctaattcaa cgaactctac
     3661 taccttgttg gtgaataacg actgctctgt tcatgcgtct ggaaacggga acgcctctac
     3721 tgagcgcaat ggcgtatcct tttccgtgca aaatggagac gtatgtctcc atgattttac
     3781 aggtaagcag cacatgttca acgagaagga agatagttgc aacggtaagg gaaggatggc
     3841 cctgaggcgg acctccaaga ggggctccct gcacttcatt gaacagatga tcgataccgg
     3901 tggacgcacc ccacccagcc tgggtcccca agatgagtcc tgcaccaccg ccagctcctc
     3961 cctggccaag gacacttcat cgaccggtga gaacctgtac ttccagctaa gattagataa
     4021 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
     4081 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa
     4141 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca
     4201 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt
     4261 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac
     4321 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc
     4381 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt
     4441 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat
     4501 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt
     4561 cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg
     4621 gtccgcgtac agccgcgcgc gtacgaaaaa caattacggg tctaccatcg agggcctgct
     4681 cgatctcccg gacgacgacg cccccgaaga ggcggggctg gcggctccgc gcctgtcctt
     4741 tctccccgcg ggacacacgc gcagactgtc gacggccccc ccgaccgatg tcagcctggg
     4801 ggacgagctc cacttagacg gcgaggacgt ggcgatggcg catgccgacg cgctagacga
     4861 tttcgatctg gacatgttgg gggacgggga ttccccgggt ccgggattta ccccccacga
     4921 ctccgccccc tacggcgctc tggatatggc cgacttcgag tttgagcaga tgtttaccga
     4981 tgcccttgga attgacgagt acggtgggta gtctagaggg cccgtttaaa cccgctgatc
     5041 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     5101 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5161 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5221 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5281 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     5341 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5401 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5461 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5521 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5581 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5641 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5701 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     5761 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5821 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5881 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5941 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6001 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     6061 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     6121 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     6181 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     6241 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     6301 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     6361 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     6421 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     6481 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     6541 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     6601 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     6661 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     6721 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     6781 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     6841 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     6901 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     6961 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     7021 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7081 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     7141 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7201 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7261 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7321 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7381 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7441 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7501 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7561 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7621 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7681 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7741 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7801 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7861 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7921 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7981 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8041 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8101 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8161 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8221 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8281 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8341 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8401 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8461 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8521 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8581 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8641 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8701 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8761 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8821 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8881 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8941 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9001 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9061 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9121 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9181 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9241 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9301 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9361 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9421 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     9481 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     9541 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     9601 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     9661 cgatgtacgg gccagatata cgcgtt
//
