LOCUS Exported 5218 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Blue-light dependent heterodimerization system. ACCESSION . VERSION . KEYWORDS Lys-eMagA-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5218) AUTHORS Benedetti L, Marvin JS, Falahati H, Guillen-Samander A, Looger LL, De Camilli P TITLE Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells. JOURNAL Elife. 2020 Nov 11;9. pii: 63230. doi: 10.7554/eLife.63230. PUBMED 33174843 REFERENCE 2 (bases 1 to 5218) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5218 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 102..305 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 256..276 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 507..516 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1017..1733 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" polyA_signal 1743..1864 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1780..1799) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1834..1853 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1871..2326) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2008..2029) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2220..2239 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2353..2457 /gene="bla" /label=AmpR promoter promoter 2459..2816 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2482..2502) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2667..2802 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2729..2748 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2851..3645 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2905..2924) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3515..3534 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3833..3852) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3877..3924 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4253..4841 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4742..4761 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 5016..101 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 61 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 121 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 181 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 241 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 301 gagctggttt agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa 361 gcttcgaatt catggggtgc tgctacagca gcgagaacga ggactcggac caggaccgag 421 aggagcggaa gctgctgctg gaccctagca gcccccctac caaagctctc aatggagccg 481 agcccaacta cccggatcca ccggtcgcca ccatgggaca cactctttac gcccctggag 541 gatacgacat tatgggatat ttggatcaga ttgcgaaccg cccaaaccct caggtcgaac 601 tggggcctgt ggacctgtca tgtgccctga tcctgtgcga tctgaagcaa aaggacactc 661 cgatcgtcta cgcctcggaa gccttcttgg agatgaccgg atacaacaga catgaggtgc 721 tcggcaggaa ctgcagattc ctgcagtccc ccgacgggat ggtgaaacca aagtcgactc 781 gcaaatatgt ggactcgaac acgatctaca ccatcaagaa ggccatcgac cggaacgccg 841 aggtccaggt ggaggtggtc aactttaaga agaacggcca gcggttcgtg aactttctga 901 ccatcattcc ggtccgggat gaaaccggag agtacagata ctccatcgga ttccagtgcg 961 aaaccgaagg cggctccgga ggcagcggcg gttctggagg ttccggtggc ggctccatgg 1021 tgtcgaaggg agaggagctt ttcaccggcg tggtgccgat tcttgtggaa ctggacggcg 1081 acgtgaacgg acataagttc agcgtgtcag gagaaggaga aggggacgcg acttacggaa 1141 agctgaccct gaagttcatc tgtaccaccg ggaagctccc ggtgccctgg ccgaccctcg 1201 tgactactct gacctacgga gtgcagtgct tctcccgcta cccggaccat atgaagcagc 1261 acgatttctt caagagcgct atgcccgagg gttacgtgca agaacggacc atcttcttca 1321 aggacgacgg aaactacaag accagagccg aagtgaaatt cgaaggagac accctcgtca 1381 atcgcatcga gctgaaggga atcgacttta aggaagatgg gaatatcctg ggtcacaagc 1441 tggagtacaa ctacaactcc cacaatgtgt acatcatggc ggataagcaa aagaacggca 1501 ttaaggtcaa cttcaagatc cggcataaca tagaagatgg ctccgtgcaa ctggcggacc 1561 actaccagca gaacaccccc attggggacg gtccggtgct gctccctgat aaccactacc 1621 tgtcaaccca aagcgcactg tccaaggacc ccaacgagaa gagggaccac atggtgctgc 1681 tggagttcgt cactgccgct ggcatcactc tcggaatgga cgagctgtac aaggggtgag 1741 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 1801 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 1861 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 1921 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 1981 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 2041 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 2101 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 2161 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 2221 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 2281 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt 2341 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 2401 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct 2461 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 2521 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 2581 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 2641 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 2701 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 2761 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc 2821 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 2881 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 2941 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 3001 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 3061 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 3121 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 3181 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 3241 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 3301 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 3361 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 3421 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 3481 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 3541 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 3601 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt 3661 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 3721 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 3781 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac 3841 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa 3901 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 3961 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac 4021 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc 4081 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat 4141 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 4201 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 4261 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 4321 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 4381 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 4441 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 4501 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 4561 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 4621 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 4681 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 4741 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 4801 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 4861 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 4921 ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg 4981 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc 5041 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc 5101 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact 5161 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtca //