LOCUS       Exported               10146 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses Rhinolophus sinicus ACE2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pscALPSpuro-RsACE2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang
            Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, 
            Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, 
            Kyratsous CA, Dudkina NV, Shen K, Luban J
  TITLE     Structural and Functional Analysis of the D614G SARS-CoV-2 Spike 
            Protein Variant.
  JOURNAL   Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032.
  PUBMED    32991842
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(240..260)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        245..574
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      425..560
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     487..506
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        586..965
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        967..1165
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1120..1140
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1183..1363
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1410..1535
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2032..2265
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2449..2493
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2791..2908
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3045..3452
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5895..5924
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6428..7027
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6428..6447)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6924..6944
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7037..7625
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7090..7110)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7508..7519)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8143..8323
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8394..8982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8474..8493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9153..10013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9776..9795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10014..10118)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgctgtg
      241 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      301 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      361 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      421 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      481 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
      541 aggaggcttt tttggaggcc taggcttttg caaaaagctc gcgttgacat tgattattga
      601 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      661 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      721 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      781 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      841 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      901 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      961 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     1021 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     1081 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     1141 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
     1201 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     1261 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     1321 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     1381 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     1441 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     1501 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     1561 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     1621 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     1681 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1741 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1801 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1861 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     1921 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1981 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     2041 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     2101 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     2161 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     2221 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     2281 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     2341 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaat taacaattac
     2401 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2461 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2521 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2581 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2641 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     2701 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     2761 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     2821 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2881 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     2941 ttggttaatt aactgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg
     3001 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg ccattttgca
     3061 aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa
     3121 aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg
     3181 gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc
     3241 ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc
     3301 caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt
     3361 ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg
     3421 cgccagtcct ccgacagact gagtcgcccg ggggtctaga agccgccacc atgagctcta
     3481 gctcatggct gctcctgagc ttggttgccg tcacgacggc acagtttacg acagaagatc
     3541 tcgcaaaaat ttttttggat gagttcaatt ccgaagcaga gaatttgtca taccaatcca
     3601 gccttgccag ctgggactat aacacaaata tcaatgatga aaacgttcaa aaaatggatg
     3661 aagcgggcgc aaaatggtcc gcgttttatg aagaacaatc caaactcgct aaaaactatc
     3721 cactggaaca aatacaaaat gtgactgtca aactgcagct gcaaatcttg caacaaagtg
     3781 gctcaccagt actttctgag gacaaaagca agcgactcaa ttcaattctg aatgcgatgt
     3841 ccaccatcta ctcaacggga aaagtttgca aaccgaacaa gccacatgag tgtttgctcc
     3901 ttgagcccgg gctggataat ataatgggta ccagcaaaga ctatagcgaa aggctgtggg
     3961 cctgggaagg gtggagggca gaggtaggaa agcagctgcg gccactgtac gaagagtatg
     4021 tcgttctcaa aaacgaaatg gctcgcgggt atcactatga agactacggg gactactgga
     4081 ggcgggatta tgaaaccgag gagtccccag ggccgggtta ctcaagggac cagctcatga
     4141 aggacgtcga gcgcatcttc acagagatca agccgctcta tgagcatctt cacgcctacg
     4201 ttcgagcgaa gcttatggac acatatccgt tccatatatc tccaacgggc tgcctgccag
     4261 cacatcttct gggcgatatg tggggtcgat tctggacgaa cctttaccct ctcacagtac
     4321 cgttcgggca aaagccgaat atagacgtta cggatgagat gctcaagcag ggttgggatg
     4381 ctgatcggat attcaaggaa gcagaaaagt tttttgtatc tgttgggctt cccaatatga
     4441 ccgaaggatt ctggaataat tccatgctta ccgagcccgg agacggtcgc aaagtggttt
     4501 gccaccctac ggcctgggat cttggtaaag gagattttcg aataaaaatg tgcactaaag
     4561 taaccatgga agacttcttg acagcgcatc atgagatggg acacatacag tacgacatgg
     4621 cctatgctag tcagccctat ctcttgcgga acggggccaa cgaaggtttc catgaagcgg
     4681 tcggcgaagt gatgtccctt tcagtagcga ctcccaaaca cttgaagact atgggacttc
     4741 tcagcccgga cttcagggaa gataatgaga ctgaaataaa ctttcttctt aagcaggccc
     4801 tcaatattgt tggtacactt ccgtttactt acatgctgga aaaatggagg tggatggtct
     4861 tcaagggcga aattccgaag gaggaatgga tgaagaaatg gtgggagatg aagcggaaga
     4921 ttgtaggggt agtagagcct gttccgcatg atgaaacata ttgtgacccg gcgtcccttt
     4981 ttcatgtcgc caatgattac agcttcatcc gctattatac gagaactatt ttcgagttcc
     5041 aatttcacga agccttgtgc aggattgctc aacatgacgg accccttcat aaatgcgata
     5101 tatccaacag tacggatgca gggaaaaagt tgcatcaaat gctctccgtg ggaaaaagcc
     5161 aagcctggac gaagacgctt gaagatatcg tagattcacg aaacatggac gttggtccac
     5221 ttctccgata ctttgaacca ctttacacgt ggctgcaaga gcagaatcgc aagtcctatg
     5281 ttggctggaa cacagattgg agtccgtaca gtgatcagtc catcaaggtg cggatctcac
     5341 tcaagtctgc gttgggggag aacgcatatg agtggaacga caacgaaatg tatcttttcc
     5401 gctcaagtgt tgcgtacgcg atgagggaat atttcctcaa agaaaaacat caaactattc
     5461 tctttggcgc tgaaaatgtc tgggtctcca atttgaaacc acgaattagc tttaactttc
     5521 atgtaaccag tcccggaaat ctctccgaca tcataccgag gcccgaggtc gaaggtgcga
     5581 tacggatgag caggagtagg attaatgacg catttcgcct ggatgacaac tccctcgagt
     5641 tccttggcat acaaccgacg ctcgggccac cctaccaacc tccggtcacg atctggctga
     5701 tcgtgtttgg cgtagttatg gcagttgttg tagtgggcat tgttgtactc atcatcacag
     5761 gcatacgaga cagaagaaaa acagaccagg caaggtcaga ggagaaccct tatagttccg
     5821 ttgaccttag taaaggtgag aataatcccg gttttcagaa cggcgacgac gtccaaacgt
     5881 catttggagc agctgagcag aagctgatca gcgaggagga cctgtaggaa ttctcgacct
     5941 tgactggcgg cgcgaccttg aggcctgcgt tcgcctcagt tgccccctct gtgcaatggg
     6001 gagacgcgcc tcatcgcttg acaacggccg aagagccgcc gcgcttccgt ctcccgcgtg
     6061 cgcgcgccat gctgcccacc cccgttccgc actgaccctc ccccgtgccc cgcgtcccgt
     6121 actgccgccc cgccccgagt cccatgccgc agccaccgcg acggagcccg caggcgggaa
     6181 cctgcctccg cgcgttagcg cgcacgcgcg cctcatgtgt cgtccccatc agcgccggct
     6241 tccgtctata ggccagatgc actgtcactc tggcgaagtc gcagacccga ttggccggga
     6301 cggaggcgcg agaccgggtt gcgggcgggg ccgaacgtgg tataaaaggg gcgggaggcc
     6361 aggctcgtgc cgttttgcag acgccaccgc cgaggaaaac cgtgtactat tagccacgcg
     6421 tgccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     6481 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6541 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6601 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6661 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6721 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6781 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6841 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6901 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6961 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     7021 tgcctgagcg atcgctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7081 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7141 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7201 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7261 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7321 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7381 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7441 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7501 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7561 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7621 cccgcttaat cgcgtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     7681 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     7741 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7801 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     7861 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     7921 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     7981 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     8041 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     8101 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     8161 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8221 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8281 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttcatgtga
     8341 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8401 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8461 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8521 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8581 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8641 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8701 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8761 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8821 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8881 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8941 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9001 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9061 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9121 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9181 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9241 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9301 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9361 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9421 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9481 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9541 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9601 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9661 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9721 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9781 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9841 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9901 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9961 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10021 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10081 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10141 cctgac
//
