LOCUS       Exported               10949 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway destination clone of GSK3B (human) tagged with N-terminal 
            TurboID-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    p663-UBC-TurboID-V5-GSK3B_IDG-K
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10949)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10949
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    148..264
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    298..318
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        318..1529
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1366..1385
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1533..1553)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1554..1563
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1560..2522
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MGKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2529..2570
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2571..2595
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3418..3429
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3901..3925)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3955..4543
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4008..4028)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4426..4437)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4557..5056
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4592..4615)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4989..5008
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5077..5676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5077..5096)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5573..5593
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5814..6047
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6119..6240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6156..6175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6210..6229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6280..6415
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6342..6361
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6435..6454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6436..6454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6464..6481)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6464..6480)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6473..6495)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6622..7077
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6709..6728)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6919..6940
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7103..7207
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7208..8068
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7426..7445)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8239..8827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8728..8747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8981..8998
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9115..9136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9151..9181
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9189..9205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9194..9216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9213..9229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9213..9229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9248..9268
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9250..9268
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9296..9522
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9523..9703
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9750..9875
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10368..10601
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10786..10830
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
       61 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
      121 gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag
      181 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
      241 acaaattaca aaattcaaaa ttttatcgtc cagtgtggtg gaattctgca gatatcacaa
      301 ctttgtatag aaaagttggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
      361 cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc
      421 ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca
      481 gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt tctttccaga
      541 gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg
      601 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc
      661 gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag
      721 tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa
      781 gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact
      841 gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct
      901 tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc
      961 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
     1021 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
     1081 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
     1141 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
     1201 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     1261 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     1321 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     1381 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     1441 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat
     1501 tctggccgtt tttggctttt ttgttagacg gccaactttt gtatacaaag tgggccacca
     1561 tgggaaaaga caatactgtg cctctgaagc tgatcgctct cctggctaat ggcgagttcc
     1621 atagtggcga acagctggga gaaaccctgg gcatgtccag ggccgctatc aacaagcaca
     1681 ttcagactct gcgcgactgg ggcgtggacg tgttcaccgt gcccggaaag ggctactctc
     1741 tgcccgagcc tatcccgctg ctgaacgcta aacagattct gggacagctg gacggcggga
     1801 gcgtggcagt cctgcctgtg gtcgactcca ccaatcagta cctgctggat cgaatcggcg
     1861 agctgaagag tggggatgct tgcattgcag aatatcagca ggcagggaga ggaagcagag
     1921 ggaggaaatg gttctctcct tttggagcta acctgtacct gagtatgttt tggcgcctga
     1981 agcggggacc agcagcaatc ggcctgggcc cggtcatcgg aattgtcatg gcagaagcgc
     2041 tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc caatgacctg tatctgcagg
     2101 atagaaagct ggcaggcatc ctggtggagc tggccggaat aacaggcgat gctgcacaga
     2161 tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt ggaggaaagc gtggtcaatc
     2221 agggctggat cacactgcag gaagcaggga ttaacctgga caggaatact ctggccgcta
     2281 cgctgatccg agagctgcgg gcagccctgg aactgttcga gcaggaaggc ctggctccat
     2341 atctgccacg gtgggagaag ctggataact tcatcaatag acccgtgaag ctgatcattg
     2401 gggacaaaga gattttcggg attagccggg ggattgataa acagggagcc ctgctgctgg
     2461 aacaggacgg agttatcaaa ccctggatgg gcggagaaat cagtctgcgg tctgccgaaa
     2521 aggctagcgg caagcccatc cccaaccccc tgctgggcct ggacagcacc acaactttgt
     2581 acaaaaaagt tggcgccacc atgtcagggc ggcccagaac cacctccttt gcggagagct
     2641 gcaagccggt gcagcagcct tcagcttttg gcagcatgaa agttagcaga gacaaggacg
     2701 gcagcaaggt gacaacagtg gtggcaactc ctgggcaggg tccagacagg ccacaagaag
     2761 tcagctatac agacactaaa gtgattggaa atggatcatt tggtgtggta tatcaagcca
     2821 aactttgtga ttcaggagaa ctggtcgcca tcaagaaagt attgcaggac aagagattta
     2881 agaatcgaga gctccagatc atgagaaagc tagatcactg taacatagtc cgattgcgtt
     2941 atttcttcta ctccagtggt gagaagaaag atgaggtcta tcttaatctg gtgctggact
     3001 atgttccgga aacagtatac agagttgcca gacactatag tcgagccaaa cagacgctcc
     3061 ctgtgattta tgtcaagttg tatatgtatc agctgttccg aagtttagcc tatatccatt
     3121 cctttggaat ctgccatcgg gatattaaac cgcagaacct cttgttggat cctgatactg
     3181 ctgtattaaa actctgtgac tttggaagtg caaagcagct ggtccgagga gaacccaatg
     3241 tttcgtatat ctgttctcgg tactataggg caccagagtt gatctttgga gccactgatt
     3301 atacctctag tatagatgta tggtctgctg gctgtgtgtt ggctgagctg ttactaggac
     3361 aaccaatatt tccaggggat agtggtgtgg atcagttggt agaaataatc aaggtcctgg
     3421 gaactccaac aagggagcaa atcagagaaa tgaacccaaa ctacacagaa tttaaattcc
     3481 ctcaaattaa ggcacatcct tggactaagg attcgtcagg aacaggacat ttcacctcag
     3541 gagtgcgggt cttccgaccc cgaactccac cggaggcaat tgcactgtgt agccgtctgc
     3601 tggagtatac accaactgcc cgactaacac cactggaagc ttgtgcacat tcattttttg
     3661 atgaattacg ggacccaaat gtcaaactac caaatgggcg agacacacct gcactcttca
     3721 acttcaccac tcaagaactg tcaagtaatc cacctctggc taccatcctt attcctcctc
     3781 atgctcggat tcaagcagct gcttcaaccc ccacaaatgc cacagcagcg tcagatgcta
     3841 atactggaga ccgtggacag accaataatg ctgcttctgc atcagcttcc aactccacct
     3901 gaccaacttt cttgtacaaa gttgtgatat ccagcacagt ggcggccgct cgacaatcaa
     3961 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4021 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4081 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4141 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4201 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4261 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4321 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     4381 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4441 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4501 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctaccgggt
     4561 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     4621 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     4681 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     4741 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     4801 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     4861 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     4921 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     4981 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     5041 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg
     5101 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     5161 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     5221 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     5281 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     5341 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     5401 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     5461 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     5521 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     5581 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     5641 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
     5701 ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt taagaccaat
     5761 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5821 gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     5881 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5941 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6001 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     6061 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     6121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6181 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6241 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     6301 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6361 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     6421 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     6481 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     6541 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6601 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6661 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6721 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6781 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6841 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6901 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6961 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7021 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     7081 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7141 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7201 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7261 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7321 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7381 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7441 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7501 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7561 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7621 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7681 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7741 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7801 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7861 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7921 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7981 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8041 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8101 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8161 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8221 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8281 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8341 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8401 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8461 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8521 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8581 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8641 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8701 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8761 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8821 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8881 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8941 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9001 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9061 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     9241 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     9301 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     9361 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     9421 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     9481 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     9541 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9601 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9661 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     9721 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     9781 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     9841 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     9901 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     9961 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    10021 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    10081 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    10141 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    10201 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
    10261 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
    10321 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    10381 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    10441 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    10501 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    10561 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    10621 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    10681 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    10741 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    10801 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    10861 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    10921 gctgtacttt ctatagtgaa tagagttag
//
