LOCUS Exported 10763 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway destination clone of GSK3B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics. ACCESSION . VERSION . KEYWORDS p663-UBC-miniTurbo-V5-GSK3B_IDG-K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10763) TITLE Major lab plasmids for proteomics-based protein-interaction and protein-proximity network generation REFERENCE 2 (bases 1 to 10763) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10763 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 164..280 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 314..334 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" promoter 334..1545 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 1382..1401 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" protein_bind complement(1549..1569) /gene="mutant version of attB" /label=attB5 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" regulatory 1570..1579 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1582..2352 /codon_start=1 /product="N-terminally truncated mutant of the E. coli biotin ligase BioID engineered for improved catalytic efficiency (Branon et al., 2018)" /label=miniTurbo /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK" CDS 2359..2400 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" protein_bind 2401..2425 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3248..3259 /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" protein_bind complement(3731..3755) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 3785..4373 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3838..3858) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4256..4267) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 4387..4886 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(4422..4445) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 4819..4838 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 4907..5506 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4907..4926) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5403..5423 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 5644..5877 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 5949..6070 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5986..6005) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6040..6059 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 6110..6245 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6172..6191 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(6265..6284) /label=T7 /note="T7 promoter, forward primer" promoter complement(6266..6284) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6294..6311) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6294..6310) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6303..6325) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 6452..6907 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6539..6558) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6749..6770 /label=F1ori-F /note="F1 origin, forward primer" promoter 6933..7037 /gene="bla" /label=AmpR promoter CDS 7038..7898 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7256..7275) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8069..8657 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8558..8577 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8811..8828 /label=L4440 /note="L4440 vector, forward primer" protein_bind 8945..8966 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8981..9011 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9019..9035 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9024..9046 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9043..9059 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9043..9059 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9078..9098 /label=T3 /note="T3 promoter, forward primer" promoter 9080..9098 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 9126..9352 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 9353..9533 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 9580..9705 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 10198..10431 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10616..10660 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc 61 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca 121 gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg 181 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa 241 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgtccagt gtggtggaat 301 tctgcagata tcacaacttt gtatagaaaa gttggcctcc gcgccgggtt ttggcgcctc 361 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc 421 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct 481 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac 541 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg 601 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg 661 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg 721 atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc 781 gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc 841 aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc 901 ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg 961 gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg 1021 gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga 1081 actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc 1141 cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat 1201 aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg 1261 cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg 1321 gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag 1381 ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt 1441 taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt 1501 aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacggcca acttttgtat 1561 acaaagtggg ccaccatggg aatcccgctg ctgaacgcta aacagattct gggacagctg 1621 gacggcggga gcgtggcagt cctgcctgtg gtcgactcca ccaatcagta cctgctggat 1681 cgaatcggcg agctgaagag tggggatgct tgcattgcag aatatcagca ggcagggaga 1741 ggaagcagag ggaggaaatg gttctctcct tttggagcta acctgtacct gagtatgttt 1801 tggcgcctga agcggggacc agcagcaatc ggcctgggcc cggtcatcgg aattgtcatg 1861 gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc caatgacctg 1921 tatctgcagg atagaaagct ggcaggcatc ctggtggagc tggccggaat aacaggcgat 1981 gctgcacaga tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt ggaggaaagc 2041 gtggtcaatc agggctggat cacactgcag gaagcaggga ttaacctgga caggaatact 2101 ctggccgcta tgctgatccg agagctgcgg gcagccctgg aactgttcga gcaggaaggc 2161 ctggctccat atctgtcacg gtgggagaag ctggataact tcatcaatag acccgtgaag 2221 ctgatcattg gggacaaaga gattttcggg attagccggg ggattgataa acagggagcc 2281 ctgctgctgg aacaggacgg agttatcaaa ccctggatgg gcggagaaat cagtctgcgg 2341 tctgccgaaa aggctagcgg caagcccatc cccaaccccc tgctgggcct ggacagcacc 2401 acaactttgt acaaaaaagt tggcgccacc atgtcagggc ggcccagaac cacctccttt 2461 gcggagagct gcaagccggt gcagcagcct tcagcttttg gcagcatgaa agttagcaga 2521 gacaaggacg gcagcaaggt gacaacagtg gtggcaactc ctgggcaggg tccagacagg 2581 ccacaagaag tcagctatac agacactaaa gtgattggaa atggatcatt tggtgtggta 2641 tatcaagcca aactttgtga ttcaggagaa ctggtcgcca tcaagaaagt attgcaggac 2701 aagagattta agaatcgaga gctccagatc atgagaaagc tagatcactg taacatagtc 2761 cgattgcgtt atttcttcta ctccagtggt gagaagaaag atgaggtcta tcttaatctg 2821 gtgctggact atgttccgga aacagtatac agagttgcca gacactatag tcgagccaaa 2881 cagacgctcc ctgtgattta tgtcaagttg tatatgtatc agctgttccg aagtttagcc 2941 tatatccatt cctttggaat ctgccatcgg gatattaaac cgcagaacct cttgttggat 3001 cctgatactg ctgtattaaa actctgtgac tttggaagtg caaagcagct ggtccgagga 3061 gaacccaatg tttcgtatat ctgttctcgg tactataggg caccagagtt gatctttgga 3121 gccactgatt atacctctag tatagatgta tggtctgctg gctgtgtgtt ggctgagctg 3181 ttactaggac aaccaatatt tccaggggat agtggtgtgg atcagttggt agaaataatc 3241 aaggtcctgg gaactccaac aagggagcaa atcagagaaa tgaacccaaa ctacacagaa 3301 tttaaattcc ctcaaattaa ggcacatcct tggactaagg attcgtcagg aacaggacat 3361 ttcacctcag gagtgcgggt cttccgaccc cgaactccac cggaggcaat tgcactgtgt 3421 agccgtctgc tggagtatac accaactgcc cgactaacac cactggaagc ttgtgcacat 3481 tcattttttg atgaattacg ggacccaaat gtcaaactac caaatgggcg agacacacct 3541 gcactcttca acttcaccac tcaagaactg tcaagtaatc cacctctggc taccatcctt 3601 attcctcctc atgctcggat tcaagcagct gcttcaaccc ccacaaatgc cacagcagcg 3661 tcagatgcta atactggaga ccgtggacag accaataatg ctgcttctgc atcagcttcc 3721 aactccacct gaccaacttt cttgtacaaa gttgtgatat ccagcacagt ggcggccgct 3781 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 3841 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 3901 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 3961 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 4021 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 4081 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 4141 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 4201 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 4261 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 4321 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 4381 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc 4441 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg 4501 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc 4561 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag 4621 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag 4681 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg 4741 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa 4801 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct 4861 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa 4921 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc 4981 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg 5041 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 5101 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 5161 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 5221 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 5281 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 5341 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 5401 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 5461 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga 5521 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt 5581 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 5641 gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt 5701 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 5761 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 5821 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 5881 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 5941 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 6001 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 6061 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct 6121 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 6181 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 6241 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc 6301 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 6361 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 6421 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg 6481 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 6541 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 6601 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 6661 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 6721 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 6781 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 6841 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt 6901 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 6961 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 7021 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 7081 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 7141 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 7201 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 7261 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 7321 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 7381 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 7441 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 7501 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 7561 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 7621 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 7681 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 7741 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 7801 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 7861 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 7921 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 7981 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 8041 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 8101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 8161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 8221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 8281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 8341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 8401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 8461 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 8521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 8581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 8641 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 8701 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 8761 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 8821 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 8881 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 8941 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 9001 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc 9061 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag 9121 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 9181 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 9241 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 9301 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 9361 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 9421 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 9481 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 9541 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg 9601 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 9661 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 9721 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 9781 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 9841 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 9901 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 9961 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 10021 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag 10081 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 10141 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 10201 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 10261 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 10321 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 10381 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 10441 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 10501 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 10561 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 10621 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 10681 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 10741 aatagttttt gctgtacttt cta //