LOCUS Exported 10319 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway destination clone of CDK10 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics. ACCESSION . VERSION . KEYWORDS p663-UBC-miniTurbo-V5-CDK10_IDG-K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10319) TITLE Major lab plasmids for proteomics-based protein-interaction and protein-proximity network generation REFERENCE 2 (bases 1 to 10319) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10319 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 135..251 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 285..305 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" promoter 305..1516 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 1353..1372 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" protein_bind complement(1520..1540) /gene="mutant version of attB" /label=attB5 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" regulatory 1541..1550 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1553..2323 /codon_start=1 /product="N-terminally truncated mutant of the E. coli biotin ligase BioID engineered for improved catalytic efficiency (Branon et al., 2018)" /label=miniTurbo /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK" CDS 2330..2371 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" protein_bind 2372..2396 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(3258..3282) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 3312..3900 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3365..3385) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3783..3794) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 3914..4413 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(3949..3972) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 4346..4365 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 4434..5033 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4434..4453) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4930..4950 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 5171..5404 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 5476..5597 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5513..5532) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5567..5586 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 5637..5772 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5699..5718 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(5792..5811) /label=T7 /note="T7 promoter, forward primer" promoter complement(5793..5811) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5821..5838) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(5821..5837) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5830..5852) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 5979..6434 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6066..6085) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6276..6297 /label=F1ori-F /note="F1 origin, forward primer" promoter 6460..6564 /gene="bla" /label=AmpR promoter CDS 6565..7425 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6783..6802) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7596..8184 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8085..8104 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8338..8355 /label=L4440 /note="L4440 vector, forward primer" protein_bind 8472..8493 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8508..8538 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8546..8562 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8551..8573 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 8570..8586 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 8570..8586 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 8605..8625 /label=T3 /note="T3 promoter, forward primer" promoter 8607..8625 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 8653..8879 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 8880..9060 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 9107..9232 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9725..9958 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10143..10187 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 61 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga 121 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga 181 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa 241 ttcaaaattt tatcgtccag tgtggtggaa ttctgcagat atcacaactt tgtatagaaa 301 agttggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc 361 gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg 421 acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt 481 ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc 541 gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc 601 cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt 661 gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg 721 ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga 781 ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg 841 agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct 901 gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg 961 ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga 1021 ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg 1081 cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg 1141 tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg 1201 tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct 1261 gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg 1321 gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc 1381 ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg 1441 gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt 1501 ggcttttttg ttagacggcc aacttttgta tacaaagtgg gccaccatgg gaatcccgct 1561 gctgaacgct aaacagattc tgggacagct ggacggcggg agcgtggcag tcctgcctgt 1621 ggtcgactcc accaatcagt acctgctgga tcgaatcggc gagctgaaga gtggggatgc 1681 ttgcattgca gaatatcagc aggcagggag aggaagcaga gggaggaaat ggttctctcc 1741 ttttggagct aacctgtacc tgagtatgtt ttggcgcctg aagcggggac cagcagcaat 1801 cggcctgggc ccggtcatcg gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga 1861 caaggtgcga gtcaaatggc ccaatgacct gtatctgcag gatagaaagc tggcaggcat 1921 cctggtggag ctggccggaa taacaggcga tgctgcacag atcgtcattg gcgccgggat 1981 taacgtggct atgaggcgcg tggaggaaag cgtggtcaat cagggctgga tcacactgca 2041 ggaagcaggg attaacctgg acaggaatac tctggccgct atgctgatcc gagagctgcg 2101 ggcagccctg gaactgttcg agcaggaagg cctggctcca tatctgtcac ggtgggagaa 2161 gctggataac ttcatcaata gacccgtgaa gctgatcatt ggggacaaag agattttcgg 2221 gattagccgg gggattgata aacagggagc cctgctgctg gaacaggacg gagttatcaa 2281 accctggatg ggcggagaaa tcagtctgcg gtctgccgaa aaggctagcg gcaagcccat 2341 ccccaacccc ctgctgggcc tggacagcac cacaactttg tacaaaaaag caggctccac 2401 catggacaag gagaaggatg gcatccccat cagcagcttg cgggagatca cgctgctgct 2461 ccgcctgcgt catccgaaca tcgtggagct gaaggaggtg gttgtgggga accacctgga 2521 gagcatcttc ctggtgatgg gttactgtga gcaggacctg gccagcctcc tggagaatat 2581 gccaacaccc ttctcggagg ctcaggtcaa gtgcatcgtg ctgcaggtgc tccggggcct 2641 ccagtatctg cacaggaact tcattatcca cagggacctg aaggtttcca acttgctcat 2701 gaccgacaag ggttgtgtga agacagcgga tttcggcctg gcccgggcct atggtgtccc 2761 agtaaagcca atgaccccca aggtggtcac tctctggtac cgagcccctg aactgctgtt 2821 gggaaccacc acgcagacca ccagcatcga catgtgggct gtgggctgca tactggccga 2881 gctgctggcg cacaggcctc ttctccccgg cacttccgag atccaccaga tcgacttgat 2941 cgtgcagctg ctgggcacgc ccagtgagaa catctggccg ggcttttcca agctgccact 3001 ggtcggccag tacagcctcc ggaagcagcc ctacaacaac ctgaagcaca agttcccatg 3061 gctgtcggag gccgggctgc gcctgctgca cttcctgttc atggcgacgg ccggggactg 3121 cctggagagc tcctatttca aggagaagcc cctaccctgt gagccggagc tcatgccgac 3181 ctttccccac caccgcaaca agcgggccgc cccagccacc tccgagggcc agagcaagcg 3241 ctgtaaaccc tgaatccacc cagctttctt gtacaaagtt gtgatatcca gcacagtggc 3301 ggccgctcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 3361 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 3421 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 3481 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 3541 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 3601 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 3661 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 3721 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 3781 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 3841 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 3901 ctggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta 3961 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca 4021 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact 4081 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa 4141 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag 4201 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag 4261 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc 4321 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg 4381 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg 4441 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc 4501 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca 4561 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 4621 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 4681 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 4741 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 4801 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 4861 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 4921 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 4981 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc 5041 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatct cgagggcccg 5101 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 5161 aaggggggac tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt 5221 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 5281 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 5341 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 5401 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 5461 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 5521 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 5581 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc 5641 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 5701 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 5761 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc 5821 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 5881 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 5941 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt 6001 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 6061 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 6121 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 6181 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 6241 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 6301 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 6361 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 6421 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 6481 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 6541 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 6601 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 6661 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 6721 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 6781 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 6841 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 6901 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 6961 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 7021 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 7081 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 7141 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 7201 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 7261 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 7321 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 7381 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 7441 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 7501 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 7561 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 7621 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 7681 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 7741 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 7801 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 7861 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 7921 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 7981 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 8041 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 8101 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 8161 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 8221 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 8281 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 8341 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt 8401 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 8461 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 8521 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc 8581 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag 8641 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga 8701 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa 8761 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa 8821 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg 8881 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 8941 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 9001 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 9061 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 9121 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 9181 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 9241 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 9301 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 9361 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 9421 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 9481 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 9541 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag 9601 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat 9661 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg 9721 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt 9781 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca 9841 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca 9901 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg 9961 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt 10021 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag 10081 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag 10141 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta 10201 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag 10261 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattca //