LOCUS Exported 1269 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression. ACCESSION . VERSION . KEYWORDS pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1269) AUTHORS Zhang , Yan; Rozsa, Marton; Bushey, Daniel; Zheng, Jihong; Reep, Daniel; Broussard, Gerard Joey; Tsang, Arthur; Tsegaye, Getahun; Patel, Ronak; Narayan, Sujatha; Li, Jing Xuan; Zhang, Rongwei; Ahrens, Misha B.; Turner, Glenn C.; Wang, Samuel S.-H.; Svoboda, Karel; Korff, Wyatt; Schreiter, Eric R.; Hasseman, Jeremy P.; Kolb, Ilya; Looger, Loren L TITLE jGCaMP8 Fast Genetically Encoded Calcium Indicators JOURNAL Janelia Research Campus. Online resource. 2020 REFERENCE 2 (bases 1 to 1269) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1269 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 560..579 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 819..840 /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(946..967) /label=EGFP-C /note="EGFP, forward primer" CDS complement(1249..1266) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" ORIGIN 1 tcacttcgct gtcatcattt gtacaaactc ttcgtagttt acctgaccat ccccatcgat 61 gtctgcttcc ctgatcattt catcaacctc ttcatctgtt aacttctctc caaggtttgt 121 catcacgtgg cgaagctctg ctgcactgat gtagccattg ccatccttat caaacacacc 181 gaacgcttct ctaatttctt cttccgtgtc cctgtatttc atttttcttg ccatcattgt 241 caggaactca gggaagtcga ttgtgccgtc accgtcggca tctacttcat tgatcatgtc 301 ctgcagctct gcttctgtgg ggttcagtcc gagagaccgc atcaccgtcc ccagctcctt 361 ggttgttatt gtcccatccc cgtccttgtc aaatagggag aaagcctctt taaattctgc 421 gatctgctct tcagtcagtt ggtccggcag gttgtactcc agcttgtgcc ccaggatgtt 481 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc 541 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc 601 ctggatgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg 661 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac 721 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc 781 ctcaccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag 841 gatgggcacc accccggtga acagctcctc gcccttgctc accatgctcc ctccggtacc 901 gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag 961 gaccatgtga tcgcgcttct cgttggggtc tttcgaaagt ttggactcca cgctcaggta 1021 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggta 1081 ggcgagctgc acgccgccgt cctcgatgtt gtggcggatg tggaagttcg ccttgatgcc 1141 gttcttctgc ttgtcggcct tgatatagac gttgatcttc aggcccatca ggcgggcgat 1201 gatcttcacg gcggtggcca cctccttgaa ggtcttcttg cgacgcgtgt gatgatggtg 1261 atgatgcat //