LOCUS Exported 10569 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Prime editing in mammalian cells. ACCESSION . VERSION . KEYWORDS pCMV-PE2-SpRY-P2A-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10569) AUTHORS Kweon J, Yoon JK, Jang AH, Shin HR, See JE, Jang G, Kim JI, Kim Y TITLE Engineered Prime Editors with PAM flexibility. JOURNAL Mol Ther. 2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 10.1016/j.ymthe.2021.02.022. PUBMED 33636398 REFERENCE 2 (bases 1 to 10569) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10569 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(250..1110) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 873..892 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1111..1215) /gene="bla" /label=AmpR promoter primer_bind complement(1294..1313) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 1485..1864 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1865..2068 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2019..2039 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2110..2129 /label=T7 /note="T7 promoter, forward primer" promoter 2110..2128 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 2136..2145 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2142..3860 /codon_start=1 /product="N-terminal portion of Streptococcus pyogenes Cas9 (Zetsche et al., 2015)" /label=Cas9(N) /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE" CDS 8418..8438 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 8451..8516 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 8535..8591 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 8592..9308 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(8637..8658) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(8898..8917) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 9245..9266 /label=EGFP-C /note="EGFP, forward primer" CDS 9317..9334 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(9357..9374) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 9363..9587 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(9658..9674) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9658..9674) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9671..9693) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9682..9698 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9706..9736) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9751..9772 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9889..9906) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10060..79) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10140..10159) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 61 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 121 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 181 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 241 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 301 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 361 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 421 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 481 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 541 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 601 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 661 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 721 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 781 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 841 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 901 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 961 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 1021 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 1081 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 1141 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 1201 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga 1261 tcgatctccc gatcccctag ggtcgactct cagtacaatc tgctctgatg ccgcatagtt 1321 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat 1381 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag 1441 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac 1501 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 1561 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 1621 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 1681 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 1741 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 1801 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 1861 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 1921 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 1981 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 2041 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagatc 2101 cgcggccgct aatacgactc actataggga gagccgccac catggacaag aagtacagca 2161 tcggcctgga catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg 2221 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc 2281 tgatcggagc cctgctgttc gacagcggcg aaacagccga gagaacccgg ctgaagagaa 2341 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca 2401 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg 2461 tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg 2521 cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg 2581 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc 2641 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc 2701 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg 2761 acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg 2821 cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg 2881 gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga 2941 gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg 3001 ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga 3061 gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg 3121 agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt 3181 acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag 3241 ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg 3301 aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca 3361 acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg 3421 aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc 3481 gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca 3541 gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt 3601 ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg 3661 tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag 3721 tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg 3781 ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg 3841 actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt 3901 tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc 3961 tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg 4021 aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag 4081 tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga 4141 tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg 4201 gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg 4261 acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc 4321 tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc 4381 tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga 4441 accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg 4501 gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc 4561 agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg 4621 aactggacat caaccggctg tccgactacg atgtggacgc tatcgtgcct cagagctttc 4681 tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga 4741 gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc 4801 tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg 4861 gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga 4921 tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg 4981 acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc 5041 ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg 5101 cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg 5161 agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc 5221 aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg aactttttca 5281 agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg 5341 gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc 5401 tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca 5461 aagagtctat cagacccaag aggaacagcg ataagctgat cgccagaaag aaggactggg 5521 accctaagaa gtacggcggc ttcctgtggc ccaccgtggc ctattctgtg ctggtggtgg 5581 ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca 5641 ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct 5701 acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg 5761 aaaacggccg gaagagaatg ctggcctctg ccaagcagct gcagaaggga aacgaactgg 5821 ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg 5881 gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg 5941 acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc 6001 tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg 6061 agaatatcat ccacctgttt accctgacca gactgggagc ccctagagcc ttcaagtact 6121 ttgacaccac catcgacccc aagcagtaca gaagcaccaa agaggtgctg gacgccaccc 6181 tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag 6241 gtgactctgg aggatctagc ggaggatcct ctggcagcga gacaccagga acaagcgagt 6301 cagcaacacc agagagcagt ggcggcagca gcggcggcag cagcacccta aatatagaag 6361 atgagtatcg gctacatgag acctcaaaag agccagatgt ttctctaggg tccacatggc 6421 tgtctgattt tcctcaggcc tgggcggaaa ccgggggcat gggactggca gttcgccaag 6481 ctcctctgat catacctctg aaagcaacct ctacccccgt gtccataaaa caatacccca 6541 tgtcacaaga agccagactg gggatcaagc cccacataca gagactgttg gaccagggaa 6601 tactggtacc ctgccagtcc ccctggaaca cgcccctgct acccgttaag aaaccaggga 6661 ctaatgatta taggcctgtc caggatctga gagaagtcaa caagcgggtg gaagacatcc 6721 accccaccgt gcccaaccct tacaacctct tgagcgggct cccaccgtcc caccagtggt 6781 acactgtgct tgatttaaag gatgcctttt tctgcctgag actccacccc accagtcagc 6841 ctctcttcgc ctttgagtgg agagatccag agatgggaat ctcaggacaa ttgacctgga 6901 ccagactccc acagggtttc aaaaacagtc ccaccctgtt taatgaggca ctgcacagag 6961 acctagcaga cttccggatc cagcacccag acttgatcct gctacagtac gtggatgact 7021 tactgctggc cgccacttct gagctagact gccaacaagg tactcgggcc ctgttacaaa 7081 ccctagggaa cctcgggtat cgggcctcgg ccaagaaagc ccaaatttgc cagaaacagg 7141 tcaagtatct ggggtatctt ctaaaagagg gtcagagatg gctgactgag gccagaaaag 7201 agactgtgat ggggcagcct actccgaaga cccctcgaca actaagggag ttcctaggga 7261 aggcaggctt ctgtcgcctc ttcatccctg ggtttgcaga aatggcagcc cccctgtacc 7321 ctctcaccaa accggggact ctgtttaatt ggggcccaga ccaacaaaag gcctatcaag 7381 aaatcaagca agctcttcta actgccccag ccctggggtt gccagatttg actaagccct 7441 ttgaactctt tgtcgacgag aagcagggct acgccaaagg tgtcctaacg caaaaactgg 7501 gaccttggcg tcggccggtg gcctacctgt ccaaaaagct agacccagta gcagctgggt 7561 ggcccccttg cctacggatg gtagcagcca ttgccgtact gacaaaggat gcaggcaagc 7621 taaccatggg acagccacta gtcattctgg ccccccatgc agtagaggca ctagtcaaac 7681 aaccccccga ccgctggctt tccaacgccc ggatgactca ctatcaggcc ttgcttttgg 7741 acacggaccg ggtccagttc ggaccggtgg tagccctgaa cccggctacg ctgctcccac 7801 tgcctgagga agggctgcaa cacaactgcc ttgatatcct ggccgaagcc cacggaaccc 7861 gacccgacct aacggaccag ccgctcccag acgccgacca cacctggtac acggatggaa 7921 gcagtctctt acaagaggga cagcgtaagg cgggagctgc ggtgaccacc gagaccgagg 7981 taatctgggc taaagccctg ccagccggga catccgctca gcgggctgaa ctgatagcac 8041 tcacccaggc cctaaagatg gcagaaggta agaagctaaa tgtttatact gatagccgtt 8101 atgcttttgc tactgcccat atccatggag aaatatacag aaggcgtggg tggctcacat 8161 cagaaggcaa agagatcaaa aataaagacg agatcttggc cctactaaaa gccctctttc 8221 tgcccaaaag acttagcata atccattgtc caggacatca aaagggacac agcgccgagg 8281 ctagaggcaa ccggatggct gaccaagcgg cccgaaaggc agccatcaca gagactccag 8341 acacctctac cctcctcata gaaaattcat caccctctgg cggctcaaaa agaaccgccg 8401 acggcagcga attcgagccc aagaagaaga ggaaagtctc gagcggaggc gactacaaag 8461 accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagggag 8521 gcggtggaag cggcgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga 8581 atcctggacc gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 8641 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 8701 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 8761 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 8821 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 8881 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 8941 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 9001 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 9061 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 9121 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 9181 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 9241 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 9301 tgtacaagtg accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc 9361 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac 9421 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg 9481 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga 9541 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga 9601 aagaaccagc tggggctcga taccgtcgac ctctagctag agcttggcgt aatcatggtc 9661 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 9721 aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt 9781 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 9841 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 9901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 9961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 10021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 10081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 10141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 10201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 10261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 10321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 10381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 10441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 10501 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 10561 ctcttgatc //