LOCUS Exported 4677 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR223-DYRK1B SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4677) AUTHORS Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA, Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM, Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA TITLE COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. JOURNAL Nature. 2010 Nov 24. ():. PUBMED 21107320 REFERENCE 2 (bases 1 to 4677) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4677 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1988..2087) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2104..2123) /label=T7 /note="T7 promoter, forward primer" promoter complement(2105..2123) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2128..2144) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2128..2144) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 2575..3366 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3459..4047 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3948..3967 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4201..4218 /label=L4440 /note="L4440 vector, forward primer" terminator 4377..4404 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 4496..4582 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 4631..4653 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4645..4662 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4646..4662 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggccgtccc accgggccat 121 ggtcccttct ctggcttccc agggccccag gagcacacgc aggtattgcc tgatgtgcgg 181 ctactgcctc ggaggctgcc cctggccttc cgggatgcaa cctcagcccc gctgcgtaag 241 ctctctgtgg acctcatcaa gacctacaag cacatcaatg aggtatacta tgcgaagaag 301 aagcggcggg cccagcaggc gccaccccag gattcgagca acaagaagga gaagaaggtc 361 ctgaaccatg gttatgatga cgacaaccat gactacatcg tgcgcagtgg cgagcgctgg 421 ctggagcgct acgaaattga ctcgctcatt ggcaaaggct cctttggcca ggtggtgaaa 481 gcctatgatc atcagaccca ggagcttgtg gccatcaaga tcatcaagaa caaaaaggct 541 ttcctgaacc aggcccagat tgagctgcgg ctgctggagc tgatgaacca gcatgacacg 601 gagatgaagt actatatagt acacctgaag cggcacttca tgttccggaa ccacctgtgc 661 ctggtatttg agctgctgtc ctacaacctg tacgacctcc tgcgcaacac ccacttccgc 721 ggcgtctcgc tgaacctgac ccggaagctg gcgcagcagc tctgcacggc actgctcttt 781 ctggccacgc ctgagctcag catcattcac tgcgacctca agcccgaaaa catcttgctg 841 tgcaacccca agcgcagcgc catcaagatt gtggacttcg gcagctcctg ccagcttggc 901 cagaggatct accagtatat ccagagccgc ttctaccgct cacctgaggt gctcctgggc 961 acaccctacg acctggccat tgacatgtgg tccctgggct gcatccttgt ggagatgcac 1021 accggagagc ccctcttcag tggctccaat gaggtcgacc agatgaaccg cattgtggag 1081 gtgctgggca tcccaccggc cgccatgctg gaccaggcgc ccaaggctcg caagtacttt 1141 gaacggctgc ctgggggtgg ctggacccta cgaaggacga aagaactcag gaaggattac 1201 cagggccccg ggacacggcg gctgcaggag gtgctgggcg tgcagacggg cgggcccggg 1261 ggccggcggg cgggggagcc gggccacagc cccgccgact acctccgctt ccaggacctg 1321 gtgctgcgca tgctggagta tgagcccgcc gcccgcatca gccccctggg ggctctgcag 1381 cacggcttct tccgccgcac ggccgacgag gccaccaaca cgggcccggc aggcagcagt 1441 gcctccacct cgcccgcgcc cctcgacacc tgcccctctt ccagcaccgc cagctccatc 1501 tccagttctg gaggctccag tggctcctcc agtgacaacc ggacctaccg ctacagcaac 1561 cgatattgtg ggggccctgg gccccctatc acagactgtg agatgaacag cccccaggtc 1621 ccaccctccc agccgctgcg gccctgggca gggggtgatg tgccccacaa gacacatcaa 1681 gcccctgcct ctgcctcgtc actgcctggg accggggccc agttaccccc ccagccccga 1741 taccttggtc gtcccccatc accaacctca ccaccacccc cggagctgat ggatgtgagc 1801 ctggtgggcg gccctgctga ctgctcccca cctcacccag cgcctgcccc ccagcacccg 1861 gctgcctcag ccctccggac tcggatgact ggaggtcgtc cacccctccc gcctcctgat 1921 gaccctgcca ctctggggcc tcacctgggc ctccgtggtg taccccagag cacagcagcc 1981 agctcgtgcc caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt 2041 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat 2101 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg 2161 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag 2221 accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca 2281 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg 2341 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt 2401 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct 2461 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg 2521 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg 2581 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc 2641 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg 2701 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg 2761 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt 2821 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca 2881 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc 2941 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc 3001 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta 3061 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat 3121 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc 3181 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc 3241 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc 3301 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc 3361 aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac 3421 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 3481 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 3541 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 3601 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 3661 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 3721 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 3781 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 3841 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 3901 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 3961 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 4021 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 4081 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 4141 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 4201 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 4261 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 4321 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa 4381 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc 4441 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg 4501 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg 4561 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca 4621 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc //