LOCUS Exported 3430 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212). ACCESSION . VERSION . KEYWORDS CBSH3- 3m shRNA3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3430) TITLE de Blas SH3- isoforms of collybistin REFERENCE 2 (bases 1 to 3430) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3430 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(1..589) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(81..100) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(760..1620) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1383..1402 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1621..1725) /gene="bla" /label=AmpR promoter rep_origin complement(1751..2206) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1888..1909) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2100..2119 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2377..2689 /label=U6 promoter /note="RNA polymerase III promoter for mouse U6 snRNA (Das et al., 1988)" primer_bind 2608..2628 /label=mU6-F /note="Mouse U6 promoter, forward primer" polyA_signal 2758..2892 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2782..2801) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2836..2855 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(2990..3010) /label=T3 /note="T3 promoter, forward primer" promoter complement(2990..3008) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3029..3045) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3029..3045) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3042..3064) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3053..3069 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3077..3107) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3122..3143 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3260..3277) /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 1741 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 1801 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 1861 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 1921 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 1981 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 2041 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 2101 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 2161 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc 2221 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagtcg 2281 acagcctgca ggaatggccg gccatcttaa ttaagaaatt taaatatgtt taaacatagg 2341 gcccaatggc gcgccaaggc catctaggcc aagcttatcc gacgccgcca tctctaggcc 2401 cgcgccggcc ccctcgcaca gacttgtggg agaagctcgg ctactcccct gccccggtta 2461 atttgcatat aatatttcct agtaactata gaggcttaat gtgcgataaa agacagataa 2521 tctgttcttt ttaatactag ctacatttta catgataggc ttggatttct ataagagata 2581 caaatactaa attattattt taaaaaacag cacaaaagga aactcaccct aactgtaaag 2641 taattgtgtg ttttgagact ataaatatcc cttggagaaa agccttgttt gtggtacagc 2701 ctgaggtcat acaacatgta tgacctccgg ctgtaccatt tttctagata atacgtagat 2761 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa 2821 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc 2881 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg 2941 tgggaggttt tttaattctt gcggccgcgg cggtacccag cttttgttcc ctttagtgag 3001 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 3061 cgctcacaat tccacacaac atacgagccg ggagcataaa gtgtaaagcc tggggtgcct 3121 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 3181 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 3241 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 3301 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 3361 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 3421 tgctggcgtt //