LOCUS Exported 837 bp ds-DNA linear SYN 12-JUN-2021 DEFINITION Bacterial expression for structure determination; may not be full ORF. ACCESSION . VERSION . KEYWORDS Py-OMPDC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 837) TITLE Structural Genomics Consortium Clones REFERENCE 2 (bases 1 to 837) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..837 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 270..289 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind 373..392 /label=T7 /note="T7 promoter, forward primer" promoter 373..391 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 392..416 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 473..490 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 500..517 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" ORIGIN 1 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg 61 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt 121 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc 181 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc 241 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc 301 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg 361 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc 421 tagaaataat tttgtttaac tttaagaang gagatatacc atgggcagca gccatcatca 481 tcatcatcac agcagcggcc tggttccgcg tggtagtatg cattttaaaa ctaaattaaa 541 aaatcgaaga aagtgaagta aatacctgtt tgtgtattgg gttggatccc gatgaagatg 601 atataaaaaa ctttatgaaa aatgaagaac aaaatggata taaaaatatc aaaaataaca 661 tgaacagtaa taataatgga atagaaaata tcatcaaaat aggaaaggaa atactattaa 721 cagatggaga aaatatacag aatttaagtg aagaagataa gtttttttat ttctttaacc 781 acttttgttt ttatataatt aataacacaa aagaatatgc tttggtatat aaaatga //