LOCUS Exported 10511 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Peredox NADH/NAD+ sensor (cytosolic). ACCESSION . VERSION . KEYWORDS pMOS023: Peredox NADH/NAD+ sensor (cytosolic) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10511) AUTHORS Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE TITLE Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology. JOURNAL Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5. PUBMED 32753572 REFERENCE 2 (bases 1 to 10511) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10511 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..181 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 228..353 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 846..1079 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1264..1308 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 1483..1983 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 1902..1920 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 1995..2390 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 2450..2567 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 2684..2987 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2988..3191 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 3142..3162 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 3214..3238 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3258..5303 /codon_start=1 /product="genetically encoded fluorescent biosensor of the cytosolic NADH-NAD+ redox state (Hung et al., 2011)" /label=Peredox /note="consists of two pH-insensitive mutant copies of the Thermus aquaticus redox-sensing repressor Rex (T-Rex) separated by circularly permuted monomeric T-Sapphire (cpmTS), a pH-resistant GFP variant" /translation="KVPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVD KDLSYFGSYGTDGVGYTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESF ELRGFFDVDPGMVGRPVRGGVIEHVDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAG IKGILNFAPVVLEVPKEVAVENVDILAGLTRLSFAILNPTWSAAGGHGFTAHNVYIMAD KQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEK RDHMVLLEFVTAAGITHGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFS VSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSA MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTK VPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVDEDLSYFGSYGTDGVG YTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESFELRGFFDVDPGMVGR PVRGGVIEHVDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAGIKGILNFAPVVLEVP KEVAVENVDFLAGLTRLSFAILNPKWREEMMGSGTGGNASDGGGSGG" CDS 5304..6014 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(5449..5467) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 5717..5736 /label=mCherry-F /note="mCherry, forward primer" protein_bind complement(6028..6052) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 6054..6095 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6137..6725 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6190..6210) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6608..6619) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6797..7030 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7108..7229 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7145..7164) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7199..7218 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 7269..7404 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7331..7350 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(7424..7443) /label=T7 /note="T7 promoter, forward primer" promoter complement(7425..7443) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7453..7470) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7453..7469) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7462..7484) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7611..8066 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7698..7717) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7908..7929 /label=F1ori-F /note="F1 origin, forward primer" promoter 8092..8196 /gene="bla" /label=AmpR promoter CDS 8197..9057 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8415..8434) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9228..9816 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9717..9736 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9970..9987 /label=L4440 /note="L4440 vector, forward primer" protein_bind 10104..10125 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10140..10170 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10178..10194 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10183..10205 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10202..10218 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10202..10218 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10237..10257 /label=T3 /note="T3 promoter, forward primer" promoter 10239..10257 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 10285..10511 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" ORIGIN 1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc 1501 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc 1561 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc 1621 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc 1681 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc 1741 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat 1801 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg 1861 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag 1921 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc 1981 cagggggtac caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag 2041 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc 2101 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt 2161 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta 2221 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg 2281 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga 2341 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc 2401 agtagtaaga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa 2461 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 2521 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca 2581 gggacagcag agatccactt tggcgccggc tcgagggggc ccggggttat taatagtaat 2641 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 2701 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 2761 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 2821 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 2881 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 2941 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 3001 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 3061 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 3121 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 3181 taagcagagc tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agcaggctgc 3241 ccaccatggt tgctagcaaa gttcctgaag cagccatttc cagactgatt acttacctga 3301 gaattctgga agagctggaa gcccaaggtg tacaccgcac cgcctccgag caactgggcg 3361 agctggccca ggtcaccgcc ttccaggttg ataaggacct gtcctacttc ggctcctacg 3421 gcactgacgg cgttggctac actgtaccgg tcctcaagag agaactgcgc catatcctcg 3481 gtctgaaccg caaatggggc ctgtgcatcg tggggatggg ccgcctggga tccgctcttg 3541 ctgattggcc tggttttggc gagagcttcg agctgcgcgg tttcttcgat gttgacccag 3601 gtatggttgg tcggccggtt cgcggtggtg ttatcgaaca cgttgatctg ctgccccagc 3661 gcgtacctgg tcgcatcgaa atcgctctgc tgacggttcc acgcgaggca gcacagaagg 3721 ctgccgacct gctggttgcc gctggcatca agggcatcct gaacttcgct ccagttgtac 3781 tggaggttcc caaggaagtg gctgttgaga acgttgacat cctggccggt ctgacgcgtc 3841 tgagctttgc cattctgaac cccacgtgga gcgcagcagg tgggcatggt ttcactgctc 3901 acaacgtgta tatcatggct gacaaacaga agaacggtat caaggccaac ttcaagatcc 3961 gccacaacat cgaagacggc ggcgttcagc tggctgatca ctaccagcag aacaccccaa 4021 tcggcgacgg ccctgttctg ctgcctgaca accactactt aagcatccag tctaaactgt 4081 ccaaagaccc aaacgagaag cgcgaccaca tggttctgct ggaatttgtt accgccgctg 4141 gtatcaccca cggcatggac gaactgtaca agggcggcac cgggggatct atggtttcca 4201 aaggcgagga actgttcact ggtgttgttc ctatcctggt tgaactggac ggcgacgtta 4261 acggccacaa attcagcgtt agcggggaag gcgaaggaga cgctacctac ggcaagctga 4321 ctctgaagtt catctgcacc actggcaagc tgcctgttcc ttggcctacc ctggttacta 4381 ccttctccta tggtgttatg gttttcgcca gatatcctga ccacatgaag cagcacgact 4441 tcttcaagag cgccatgcct gagggctacg ttcaggaacg caccatcttt ttcaaggacg 4501 acggcaacta taagacacgc gctgaggtta agttcgaggg cgacactctg gttaaccgca 4561 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctgggccat aagcttgaat 4621 ataacaccaa ggtacctgaa gccgccatca gccgcctcat cacttacctc cgcatcctgg 4681 aggagctcga agcccaaggc gttcaccgca ctgcaagcga gcagctgggc gaactggcac 4741 aggttacagc attccaggtt gacgaagatc tgtcctattt cggttcctac ggcaccgacg 4801 gtgttggcta cacagtccct gtcctgaagc gcgagctgcg ccacatcctg ggcctgaacc 4861 gcaagtgggg cttatgcatc gttggcatgg gccgcctcgg tagtgcactg gcagactggc 4921 cgggctttgg tgaatctttc gaactgcgcg gcttcttcga cgttgatcca ggcatggttg 4981 ggcgccctgt tcgcggtggc gttatcgagc atgttgacct cctgccacag cgcgtcccgg 5041 gtcgcatcga gatcgccctg ctgaccgttc ctcgcgaagc tgcacagaag gcagccgact 5101 tactagtcgc cgctggtatc aagggaattc tgaacttcgc ccctgttgtt ctggaggttc 5161 ctaaggaagt tgctgttgaa aacgttgatt tcctggccgg cctgacccgc ctgagcttcg 5221 caatcctgaa tcctaagtgg cgcgaagaaa tgatgggttc cggaactggg ggcaacgcct 5281 ctgacggtgg tgggtctggt ggtatggtga gcaagggcga ggaggataac atggccatca 5341 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 5401 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 5461 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 5521 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 5581 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 5641 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 5701 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 5761 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 5821 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 5881 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 5941 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 6001 aactgtacaa gtgagtcgag aagcttaacc cagctttctt gtacaaagtg gttggtaagc 6061 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 6121 ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 6181 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 6241 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 6301 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 6361 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 6421 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 6481 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc 6541 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 6601 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 6661 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 6721 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 6781 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt 6841 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 6901 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 6961 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 7021 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca 7081 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 7141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 7201 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 7261 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 7321 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 7381 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta 7441 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 7501 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 7561 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg 7621 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 7681 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 7741 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 7801 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 7861 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 7921 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 7981 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 8041 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc 8101 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 8161 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 8221 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 8281 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 8341 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 8401 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 8461 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 8521 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 8581 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 8641 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 8701 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 8761 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 8821 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 8881 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 8941 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 9001 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 9061 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 9121 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 9181 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 9241 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 9301 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 9361 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 9421 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 9481 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 9541 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 9601 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 9661 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 9721 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 9781 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 9841 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 9901 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 9961 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 10021 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 10081 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 10141 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 10201 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa 10261 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca 10321 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat 10381 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat 10441 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc 10501 gatacataaa c //