LOCUS Exported 14085 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION RUBY under the control of At2S3 promoter/marker for Arabidopsis transgenes. ACCESSION . VERSION . KEYWORDS At2S3:RUBY SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14085) AUTHORS He Y, Zhang T, Sun H, Zhan H, Zhao Y TITLE A reporter for noninvasively monitoring gene expression and plant transformation. JOURNAL Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. PUBMED 33024566 REFERENCE 2 (bases 1 to 14085) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14085 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 178..202 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 2152..2208 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 3043..3099 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" promoter 5306..5485 /label=NOS promoter /note="nopaline synthase promoter" promoter 5338..5521 /function="NOS promoter" /label=NOS promoter /note="nopaline synthase promoter" terminator 6636..6888 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(7463..7480) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7463..7479) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7472..7494) /label=M13/pUC Forward /note="In lacZ gene" misc_feature 7701..7725 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 8246..9037 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 9283..9871 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9772..9791 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10025..10042 /label=L4440 /note="L4440 vector, forward primer" misc_feature 10057..10197 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(10123..10142) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 10283..10305 /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 10541..10735 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(10801..11874) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" CDS complement(12303..12932) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" ORIGIN 1 actatagcag cggaggggtt ggatcaaagt actttaaagt actttaaagt actttaaagt 61 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa 121 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt 181 tacccgccaa tatatcctgt caaacactga tagttttaag ctggcacaac tatatttcca 241 acatcactag ctaccatcaa aagattgact tctcatctta ctcgattgaa accaaattaa 301 catagggttt ttatttaaat aaaagtttaa ccttcttttt aaaaaattgt tcatagtgtc 361 atgtcagaac aagagctaca aatcacacat agcatgcata agcggagcta tgatgagtgg 421 tattgttttg ttcgtcactt gtcactcttt tccaacacat aatcccgaca acaacgtaag 481 agcatctctc tctctccaca cacactcatg catgcatgca ttcttacacg tgattgccat 541 gcaaatctcc tttctcacct ataaatacaa accaaccctt cactacactc ttcactcaaa 601 ccaaaacaag aaaacataca caaatagcaa aacggtacca acaaaactag tatggatcat 661 gcgaccctcg ccatgatcct cgcgatctgg ttcatcagct tccacttcat caagctgctg 721 ttctcccagc agaccaccaa gctgcttccg ccaggaccaa agccgcttcc gatcatcggc 781 aacatccttg aggtgggcaa gaagccgcat cggtccttcg ccaacctcgc caagattcac 841 ggcccactca tttccctcag actcggctct gtgaccacca tcgttgtgtc ctctgccgac 901 gtggccaaag agatgttcct caagaaggat cacccgctct ccaaccgcac gatcccgaat 961 agtgttacag ccggcgacca ccacaagctc accatgtctt ggctcccggt gtctccgaag 1021 tggcgcaact tccgcaagat taccgccgtg catctgctct ccccacagag actcgatgcc 1081 tgccagacat tcaggcacgc caaggtgcag cagctctacg agtacgttca agagtgcgcc 1141 cagaaaggcc aggccgtgga tattggcaag gccgccttta cgaccagcct caacctcctc 1201 agcaagctgt tcttcagcgt cgagctggcg caccacaagt cccataccag ccaagagttc 1261 aaagagctga tctggaacat catggaagat ataggcaagc cgaactacgc cgactacttc 1321 ccgattctcg gctgcgttga cccatctggc attagaagaa ggctcgcctg ctccttcgac 1381 aagctgatcg ccgtgttcca gggcatcatc tgcgagagac tcgccccaga ttcctccacc 1441 acaactacca ccaccaccga cgacgtgctc gatgtgctcc tccagctgtt caagcagaac 1501 gagctgacga tgggcgagat caaccacctc ctcgtggaca tcttcgacgc cggcaccgat 1561 accacatcct ccacattcga gtgggtgatg accgagctga tccgcaatcc agagatgatg 1621 gaaaaggccc aagaggaaat caagcaggtc ctcggcaagg acaagcagat ccaagagtcc 1681 gacatcatca acctgccgta cctccaggcg atcatcaaag agacactccg cctccatccg 1741 ccgaccgtgt tcttgctccc aagaaaggcc gacaccgatg tcgagctgta cggctacatc 1801 gtgccgaagg atgcccagat cctcgtgaac ctctgggcca ttggcaggga cccaaacgcc 1861 tggcagaacg ccgatatttt cagcccagag cgcttcatcg gctgcgagat cgatgttaag 1921 ggccgcgatt tcggcctcct tccatttggc gctggccgca gaatttgccc aggcatgaat 1981 ctcgccatca ggatgctcac cctcatgctc gccacactcc tccagttctt caactggaag 2041 ctcgaaggcg acatctcccc gaaggacctc gacatggacg agaagttcgg cattgcgctc 2101 caaaagacca agccgctcaa gctcatcccg attccgcgct acggtagcgg agctaccaat 2161 tttagcctcc ttaagcaggc aggtgatgta gaagagaacc ccgggcctat gaagatgatg 2221 aacggcgagg acgccaacga ccagatgatc aaagagtcct tcttcatcac ccacggcaac 2281 ccgatcctca ccgtcgagga tacacatccg ctcaggccgt tcttcgagac atggcgcgag 2341 aagattttct ccaagaagcc gaaggccatc ctcatcatct ccggccactg ggagacagtg 2401 aagccaaccg tgaacgccgt gcacatcaac gacaccatcc acgacttcga cgactaccca 2461 gccgccatgt accagttcaa gtacccagct ccaggcgagc cagagcttgc gagaaaggtg 2521 gaagagatcc tcaagaagtc cgggttcgag acagccgaga cagaccaaaa gaggggcctt 2581 gatcacggcg cctgggttcc actcatgctc atgtatccag aggcggacat cccggtgtgc 2641 cagctctcag ttcagccaca tctcgacggc acctaccact acaatctcgg cagagccctc 2701 gcgccgctca agaatgatgg cgtgctcatt attggctccg gcagcgccac acatccactc 2761 gatgagacac cgcactactt cgatggtgtt gccccttggg ccgctgcctt cgattcttgg 2821 cttaggaagg ccctcatcaa cggccgcttc gaggaagtga acatctacga gagcaaggcc 2881 ccgaactgga agctcgccca tccatttcca gagcacttct acccgctcca cgttgtgctc 2941 ggcgctgctg gtgaaaagtg gaaggccgag ctgatccact cctcctggga tcatggcaca 3001 ctttgccacg gctcctacaa gttcacctcc gccggatccg gagcaaccaa ctttagcctg 3061 ctcaagcaag caggagatgt tgaggaaaat cctggcccca tgaccgccat caagatgaac 3121 accaacggcg agggcgagac acagcacatc ctcatgatcc cgttcatggc gcagggccac 3181 ctcaggccat ttctcgaact cgccatgttc ctctacaagc gctcccacgt gatcatcacc 3241 ctgctcacaa ctccgctcaa tgccggcttc ctcaggcacc tccttcacca ccattcctac 3301 tcctccagcg gcatcaggat cgtcgagctg ccattcaact ccaccaacca cggactccca 3361 ccgggcatcg agaacaccga taagctcaca ctcccgctcg tggtgtccct cttccattcc 3421 accatcagcc tcgatccgca cctccgcgat tacatctcca ggcatttcag cccagccagg 3481 ccaccactct gcgtgatcca tgatgtgttc ctcggctggg ttgaccaggt ggccaaggat 3541 gtgggctcta caggcgtggt gttcacaaca ggcggcgctt atggcacatc cgcctacgtg 3601 tccatctgga acgatctccc gcaccagaac tactccgacg accaagagtt cccgctgcca 3661 ggcttcccag agaaccataa gttccgcagg tcccagctcc atcggttcct cagatatgcc 3721 gacggctccg acgattggtc caagtatttc cagccgcagc tccgccagtc catgaagtct 3781 tttggctggc tctgcaactc cgtggaagag atcgagacac tcggcttctc catcctccgc 3841 aactacacca agctgccgat ctggggcatc ggcccactta ttgcttcccc agtgcagcac 3901 tcctcctccg acaacaattc aacaggcgcc gagttcgtgc agtggctcag cctcaaagag 3961 ccggactccg tcctctacat ctccttcggc tcccagaaca cgatcagccc gacgcagatg 4021 atggaactcg ctgctggcct tgagtcctcc gagaagccat tcctctgggt gatcagagcc 4081 ccgttcggct tcgacatcaa cgaagagatg cgcccagagt ggctgccaga gggctttgag 4141 gaacgcatga aggtgaagaa acagggcaag ctcgtgtaca agctcggccc gcagcttgag 4201 atcctcaacc atgaatccat cggcggcttt ctcacccact gcggatggaa cagcatcctt 4261 gagtctcttc gcgagggcgt tccgatgctt ggatggccac ttgctgccga gcaggcctac 4321 aacctcaagt acctcgaaga tgagatgggc gtcgcggttg agcttgctag aggcctcgaa 4381 ggcgagatct ccaaagagaa ggtcaagcgc atcgtcgaga tgatccttga gcgcaacgag 4441 ggctccaaag gctgggagat gaagaatcgc gccgtggaaa tgggcaaaaa gctcaaggac 4501 gccgtgaacg aggaaaaaga gctgaagggc tcctccgtga aggcgatcga cgatttcctc 4561 gacgccgtca tgcaggccaa acttgagcca agcctccagt gatagtgaat atgaagatga 4621 agatgaaata tttggtgtgt caaataaaaa ggttgtgtgc ttaagtttgt gtttttttct 4681 tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aacatctcat 4741 tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttctc 4801 cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta aagataagac 4861 ctaactgagt aagctagctt gttcgagtat tatggcattg ggaaaactgt ttttcttgta 4921 ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga 4981 aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt 5041 aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat aagagatatg 5101 caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat 5161 atgaggagta aaacactaga tccccaaaca agcttgaaac tgaaggcggg aaacgacaat 5221 ctgatcatga gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca 5281 agccgtttta cgtttggaac tgacagaacc gcaacgttga aggagccact cagccgcggg 5341 tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt 5401 cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc 5461 ataaattccc ctcggtatcc aattagagtc tcatattcac tctcaatcca aataatctgc 5521 accggatccg ctagaggatc tcgacctgca agatcccggg gggcaatgag atatgaaaaa 5581 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc 5641 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atataggagg 5701 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt 5761 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt 5821 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct 5881 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc 5941 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca 6001 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca 6061 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct 6121 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa 6181 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg 6241 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga 6301 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg 6361 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt 6421 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac 6481 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga 6541 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgggatctt ggaggtgatg 6601 taacatgatc acaagctgat cccccgaatt tccccgatcg ttcaaacatt tggcaataaa 6661 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga 6721 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt 6781 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg 6841 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattgatcc 6901 cccctcgaca gcttgcatgc cagcttgggc tgcaggtcga cgcgtcaatt gatatcgatc 6961 caggcctccc agctttcgtc cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca 7021 tcagtttcat tgcccacaca ccagaatcct actaagtttg agtattatgg cattggaaaa 7081 gctgttttct tctatcattt gttctgcttg taatttactg tgttctttca gtttttgttt 7141 tcggacatca aaatgcaaat ggatggataa gagttaataa atgatatggt ccttttgttc 7201 attctcaaat tattattatc tgttgttttt actttaatgg gttgaattta agtaagaaag 7261 gaactaacag tgtgatatta aggtgcaatg ttagacatat aaaacagtct ttcacctctc 7321 tttggttatg tcttgaattg gtttgtttct tcacttatct gtgtaatcaa gtttactatg 7381 agtctatgat caagtaatta tgcaatcaag ttaagtacag tataggcttt ttgtgtcgag 7441 ggggtaccga gtcgaggaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 7501 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 7561 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgggtacc 7621 gagctcgaat tcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta 7681 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag 7741 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg 7801 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta 7861 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag 7921 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca 7981 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa 8041 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc 8101 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg 8161 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag 8221 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca 8281 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca 8341 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt 8401 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga 8461 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt 8521 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg 8581 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc 8641 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga 8701 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct 8761 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg 8821 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc 8881 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct 8941 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta 9001 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa 9061 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg 9121 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc 9181 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt 9241 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 9301 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 9361 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 9421 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 9481 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 9541 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 9601 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 9661 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 9721 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 9781 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 9841 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 9901 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 9961 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 10021 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac 10081 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc 10141 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc 10201 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct 10261 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 10321 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc 10381 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc 10441 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg 10501 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt 10561 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt 10621 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta 10681 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa 10741 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca 10801 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg 10861 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag 10921 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat 10981 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag 11041 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg 11101 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc 11161 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga 11221 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt 11281 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg 11341 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt 11401 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc 11461 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg 11521 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc 11581 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa 11641 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg 11701 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc 11761 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc 11821 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat 11881 tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg 11941 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt 12001 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt 12061 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc 12121 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac 12181 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc 12241 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta 12301 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg 12361 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg 12421 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc 12481 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt 12541 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg 12601 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca 12661 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc 12721 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc 12781 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg 12841 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg 12901 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta 12961 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc 13021 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca 13081 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg 13141 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa 13201 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc 13261 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct 13321 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg 13381 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg 13441 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc 13501 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc 13561 aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg 13621 ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag 13681 gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg 13741 taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc 13801 ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg 13861 gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc 13921 gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag 13981 aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat 14041 gtcgttttca gaagacggct gcactgaacg tcagaagccg actgc //