LOCUS Exported 8798 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION 3rd gen lentiviral eGFP expression vector, CMV promoter, Neo. ACCESSION . VERSION . KEYWORDS pLenti CMV GFP Neo (657-2) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8798) AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. TITLE A versatile viral system for expression and depletion of proteins in mammalian cells. JOURNAL PLoS One. 2009 Aug 6;4(8):e6529. PUBMED 19657394 REFERENCE 2 (bases 1 to 8798) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8798 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 58..162 /gene="bla" /label=AmpR promoter CDS 163..1023 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(381..400) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1194..1782 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1683..1702 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1936..1953 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2070..2091 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2106..2136 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2144..2160 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2149..2171 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2168..2184 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2168..2184 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2203..2223 /label=T3 /note="T3 promoter, forward primer" promoter 2205..2223 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 2251..2477 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 2478..2658 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2705..2830 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3323..3556 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3741..3785 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4052..4168 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 4191..4494 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4495..4698 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4649..4669 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 4695..4719 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 4788..4797 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4794..5513 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 4794..5510 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4839..4860) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5100..5119) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5447..5468 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5529..6117 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5582..5602) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6000..6011) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 6160..6659 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(6195..6218) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 6592..6611 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 6673..7476 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6736..6755) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7346..7365 /label=Neo-F /note="Neomycin resistance gene, forward primer" LTR 7567..7800 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7872..7993 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7909..7928) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7963..7982 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8033..8168 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8095..8114 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(8188..8207) /label=T7 /note="T7 promoter, forward primer" promoter complement(8189..8207) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8217..8234) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8217..8233) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8226..8248) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 8375..32 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8462..8481) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8672..8693 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc 61 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 121 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 181 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 241 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 301 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 361 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 421 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 481 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 541 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 601 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 661 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 721 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 781 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 841 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 901 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 961 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 1021 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 1081 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 1141 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 1201 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 1261 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 1321 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 1381 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 1441 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 1501 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 1561 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 1621 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 1681 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 1741 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 1801 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 1861 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 1921 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 1981 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 2041 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 2101 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 2161 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa 2221 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt 2281 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat 2341 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc 2401 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc 2461 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg 2521 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 2581 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 2641 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga 2701 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg 2761 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg 2821 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag 2881 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga 2941 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg 3001 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt 3061 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga 3121 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct 3181 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag 3241 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga 3301 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa 3361 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta 3421 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac 3481 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa 3541 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg 3601 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct 3661 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag 3721 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa 3781 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga 3841 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag 3901 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg 3961 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat 4021 tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt 4081 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac 4141 aaaaacaaat tacaaaattc aaaattttat cgataagctt gggagttccg cgttacataa 4201 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata 4261 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag 4321 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc 4381 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta 4441 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg 4501 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt 4561 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca 4621 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag 4681 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc 4741 tgttttgacc tccatagaag acaccgactc tagaggatcc accggtcgcc accatggtga 4801 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 4861 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc 4921 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 4981 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg 5041 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 5101 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 5161 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 5221 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca 5281 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact 5341 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 5401 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 5461 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc 5521 gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 5581 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 5641 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 5701 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 5761 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 5821 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 5881 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat 5941 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 6001 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 6061 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 6121 gaattctgca gatatccgga ctagtgatct aattctaccg ggtaggggag gcgcttttcc 6181 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg 6241 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg 6301 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc 6361 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga 6421 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc 6481 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca 6541 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca 6601 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga 6661 cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc 6721 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 6781 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 6841 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 6901 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 6961 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 7021 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 7081 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 7141 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 7201 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 7261 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 7321 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 7381 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 7441 atcgccttct atcgccttct tgacgagttc ttctgagggg atccgtcgag ggcccggtac 7501 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 7561 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg 7621 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 7681 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 7741 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 7801 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 7861 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 7921 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 7981 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc 8041 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 8101 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 8161 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg 8221 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 8281 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 8341 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc 8401 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 8461 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 8521 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 8581 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 8641 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 8701 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 8761 tggttaaaaa atgagctgat ttaacaaaaa tttaacgc //