LOCUS       Exported                8426 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLV.PGK.mFoxa2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Pfisterer U, Kirkeby A, Torper O, Wood J, Nelander J, Dufour A, 
            Bjorklund A, Lindvall O, Jakobsson J, Parmar M
  TITLE     Direct conversion of human fibroblasts to dopaminergic neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8. Epub 2011 Jun
            6.
  PUBMED    21646515
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        28..132
                     /gene="bla"
                     /label=AmpR promoter
     CDS             133..993
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(351..370)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1164..1752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1653..1672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1906..1923
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2040..2061
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2076..2106
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2114..2130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2119..2141
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2138..2154
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2138..2154
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2173..2193
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2175..2193
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2294..2673
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2674..2877
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2828..2848
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2892..3072
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3119..3244
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3737..3970
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4155..4199
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4466..4583
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4629..5139
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5058..5076
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(6587..6607)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7005..7016)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7165..7398
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7470..7591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7507..7526)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7561..7580
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7631..7766
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7693..7712
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7786..7805)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7787..7805)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7815..7832)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7815..7831)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7824..7846)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7973..2
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8060..8079)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8270..8291
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
       61 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      121 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      181 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      241 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
      301 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      361 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      421 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      481 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      541 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      601 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      661 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      721 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
      781 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
      841 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
      901 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
      961 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1021 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1081 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1261 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1321 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1381 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1441 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1501 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1561 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1621 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1681 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1741 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1801 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1861 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1921 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1981 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2041 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     2101 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     2161 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttgg
     2221 ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca
     2281 ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     2341 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     2401 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2461 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     2521 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     2581 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     2641 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     2701 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     2761 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     2821 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     2881 ttagtgaacc ggggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     2941 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3001 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3061 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     3121 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     3181 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     3241 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     3301 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     3361 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     3421 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     3481 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     3541 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     3601 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     3661 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     3721 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     3781 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     3841 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     3901 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     3961 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     4021 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     4081 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     4141 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     4201 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     4261 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     4321 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     4381 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     4441 acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg
     4501 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     4561 aaattacaaa aattcaaaat tttatcgatc acgagactag cctcgagaag cttgatatcg
     4621 aattccacgg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
     4681 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
     4741 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
     4801 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
     4861 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
     4921 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
     4981 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
     5041 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
     5101 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatccatg ctgggagccg
     5161 tgaagatgga agggctcgag ccatccgact ggagcagcta ctacgcggag cccgagggct
     5221 actcttccgt gagcaacatg aacgccggcc tggggatgaa tggcatgaac acatacatga
     5281 gcatgtccgc ggctgccatg ggcggcggtt ccggcaacat gagcgcgggc tccatgaaca
     5341 tgtcatccta tgtgggcgct ggaatgagcc cgtcgctagc tggcatgtcc ccgggcgccg
     5401 gcgccatggc gggcatgagc ggctcagccg gggcggccgg cgtggcgggc atgggacctc
     5461 acctgagtcc gagtctgagc ccgctcgggg gacaggcggc cggggccatg ggtggccttg
     5521 ccccctacgc caacatgaac tcgatgagcc ccatgtacgg gcaggccggc ctgagccgcg
     5581 ctcgggaccc caagacatac cgacgcagct acacacacgc caaacctccc tactcgtaca
     5641 tctcgctcat caccatggcc atccagcaga gccccaacaa gatgctgacg ctgagcgaga
     5701 tctatcagtg gatcatggac ctcttccctt tctaccggca gaaccagcag cgctggcaga
     5761 actccatccg ccactctctc tccttcaacg actgctttct caaggtgccc cgctcgccag
     5821 acaagcctgg caagggctcc ttctggaccc tgcacccaga ctcgggcaac atgttcgaga
     5881 acggctgcta cctgcgccgc cagaagcgct tcaagtgtga gaagcaactg gcactgaagg
     5941 aagccgcggg tgcggccagt agcggaggca agaagaccgc tcctgggtcc caggcctctc
     6001 aggctcagct cggggaggcc gcgggctcgg cctccgagac tccggcgggc accgagtccc
     6061 cccattccag cgcttctccg tgtcaggagc acaagcgagg tggcctaagc gagctaaagg
     6121 gagcacctgc ctctgcgctg agtcctcccg agccggcgcc ctcgcctggg cagcagcagc
     6181 aggctgcagc ccacctgctg ggcccacctc accacccagg cctgccacca gaggcccacc
     6241 tgaagcccga gcaccattac gccttcaacc accccttctc tatcaacaac ctcatgtcgt
     6301 ccgagcagca acatcaccac agccaccacc accatcagcc ccacaaaatg gacctcaagg
     6361 cctacgaaca ggtcatgcac tacccagggg gctatggttc ccccatgcca ggcagcttgg
     6421 ccatgggccc agtcacgaac aaagcgggcc tggatgcctc gcccctggct gcagacactt
     6481 cctactacca aggagtgtac tccaggccta ttatgaactc atcctaagtc gacaatcaac
     6541 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6601 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6661 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6721 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6781 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6841 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6901 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     6961 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7021 cggaccttcc ttcccgcggc ctgctgccgg ctctagagcc tcttccgcgt cttcgccttc
     7081 ccgggtcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7141 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7201 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7261 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7321 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7381 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7441 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7501 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7561 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7621 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7681 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7741 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     7801 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7861 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7921 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     7981 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8041 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8101 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8161 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8221 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8281 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8341 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8401 tttaacaaaa tattaacgct tacaat
//
