LOCUS Exported 8890 bp ds-DNA circular SYN 22-JUN-2023 DEFINITION 3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259). ACCESSION . VERSION . KEYWORDS pMDLg/pRRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8890) AUTHORS Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L TITLE A third-generation lentivirus vector with a conditional packaging system. JOURNAL J Virol. 1998 Nov . 72(11):8463-71. PUBMED 9765382 REFERENCE 2 (bases 1 to 8890) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 22, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8890 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 363..382 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 616..633 /label=L4440 /note="L4440 vector, forward primer" polyA_signal 972..1366 /label=-beta-globin poly(A) /note="human beta-globin polyadenylation signal" misc_feature 1597..1830 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS complement(1855..4866) /codon_start=1 /gene="pol" /product="pol protein from human immunodeficiency virus 1" /label=HIV-1 pol /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ MAGDDCVASRQDED" misc_feature 2053..2170 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" CDS complement(4659..6161) /codon_start=1 /gene="gag" /product="gag protein from human immunodeficiency virus 1" /label=HIV-1 gag /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ" regulatory 6158..6167 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" intron 6245..6720 /label=-beta-globin intron /note="internally truncated intron from human beta-globin" primer_bind complement(6833..6857) /label=LNCX /note="Human CMV promoter, forward primer" promoter complement(6854..7057) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(6883..6903) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 7058..7437 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind 7539..7558 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7628..7732 /gene="bla" /label=AmpR promoter CDS 7733..8593 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7951..7970) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8764..462 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 61 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 121 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 181 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 241 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 301 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 361 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 421 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 481 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 541 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 601 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcag atctgcggcc gcactagtgc 661 tagactgcca tgtcgaggga ttccgggtca ctgtgagtgg gggaggcagg gaagaagggc 721 tcacaggaca gtcaaaccat gccccctgtt tttccttctt caagtagacc tctataagac 781 aacagagaca actaaggctg agtggccagg cgaggagaaa ccatctcgcc gtaaaacatg 841 gaaggaacac ttcaggggaa aggtggtatc tctaagcaag agaactgagt ggagtcaagg 901 ctgagagatg caggataagc aaatgggtag tgaaaagaca ttcatgagga cagctaaaac 961 aataagtaat gtaaaataca gcatagcaaa actttaacct ccaaatcaag cctctacttg 1021 aatccttttc tgagggatga ataaggcata ggcatcaggg gctgttgcca atgtgcatta 1081 gctgtttgca gcctcacctt ctttcatgga gtttaagata tagtgtattt tcccaaggtt 1141 tgaactagct cttcatttct ttatgtttta aatgcactga cctcccacat tcccttttta 1201 gtaaaatatt cagaaataat ttaaatacat cattgcaatg aaaataaatg ttttttatta 1261 ggcagaatcc agatgctcaa ggcccttcat aatatccccc agtttagtag ttggacttag 1321 ggaacaaagg aacctttaat agaaattgga cagcaagaaa gcgagcttag tgatacttgt 1381 gggccagggc attagccaca ccagccacca ctttctgata ggcagcctgc actggtgggg 1441 tgaattccgc ggaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg 1501 tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg 1561 gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat 1621 ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac 1681 agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac 1741 aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct 1801 gctcccaaga acccaaggaa caaagctcct gcggccgctc cggaattcca tgtgttaatc 1861 ctcatcctgt ctacttgcca cacaatcatc acctgccatc tgttttccat aatccctgat 1921 gatctttgct tttcttcttg gcactacttt tatgtcacta ttatcttgta ttactactgc 1981 cccttcacct ttccagagga gctttgctgg tcctttccaa actggatctc tgctgtccct 2041 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat 2101 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt 2161 tcttttaaaa ttgtggatga atactgccat ttgtactgct gtcttaagat gttcagcctg 2221 atctcttacc tgtcctataa ttttctttaa ttctttattc atagattcta ttactccttg 2281 actttgggga ttgtagggaa tgccaaattc ctgcttgatc cccgcccacc aacaggcggc 2341 cttaactgta gtactggtga aattgctgcc attgtctgta tgtactgttt ttactggcca 2401 tcttcctgct aattttaaga ggaagtatgc tgtttcttgc cctgtctctg ctggaattac 2461 ttctgcttct atatatccac tggctacatg aactgctacc aagataactt ttccttctaa 2521 atgtgtacaa tctagctgcc atattcctgg gctacagtct acttgtccat gcatggcttc 2581 cccttttagc tgacatttat cacagctggc tactatttct tttgctacta caggtggtag 2641 gttaaaatca ctagccattg ctctccaatt actgtgatat ttctcatgtt cttcttgggc 2701 cttatctatt ccatctaaaa atagtacttt cctgattcca gcactgacca atttatctac 2761 ttgttcattt cctccaattc ctttgtgtgc tggtacccat gccaggtaga ctttttcctt 2821 ttttattaac tgctctatta tttgactgac taactctgat tcactcttat ctggttgtgc 2881 ttgaatgatt cccaatgcat attgtgagtc tgtcactatg tttacttcta atcccgaatc 2941 ctgcaaagct agatgaattg cttgtaactc agtcttctga tttgttgtgt ccgttagggg 3001 gacaactttt tgtcttcctc tgtcagttac atatcctgct tttcctaatt tagtttccct 3061 attggctgcc ccatctacat agaaagtttc tgctcctatt atgggttctt tctctaactg 3121 gtaccataac ttcactaagg gaggggtatt gacaaactcc cactcaggaa tccaggtggc 3181 ttgccaatac tctgtccacc atgcttccca tgtttccttt tgtatgggta atttaaattt 3241 aggagtcttt ccccatatta ctatgctttc tgtggctatt ttttgtactg cctctgttaa 3301 ttgtttcaca tcattagtgt gggcaccctt cattcttgca tactttcctg ttttcagatt 3361 tttaaatggc tcttgataaa tttgatatgt ccattggcct tgcccctgct tctgtatttc 3421 tgctattaag tcttttgatg ggtcataata cactccatgt accggttctt ttagaatctc 3481 cctgttttct gccagttcta gctctgcttc ttctgttagt ggtactactt ctgttagtgc 3541 tttggttccc ctaagaagtt tacataattg ccttacttta atccctgcat aaatctgact 3601 tgcccaattc aattttccca ctaatttctg tatgtcattg acagtccagc tgtccttttc 3661 tggcagcact ataggctgta ctgtccattt atcaggatgg agttcataac ccatccaaag 3721 gaatggaggt tctttctgat gttttttgtc tggtgtggta aatccccacc tcaacagatg 3781 ttgtctcagt tcctctattt ttgttctatg ctgccctatt tctaagtcag atcctacata 3841 caaatcatcc atgtattgat agatgactat gtctggattt tgttttctaa aaggctctaa 3901 gatttttgtc atgctacact ggaatattgc tggtgatcct ttccatccct gtggaagcac 3961 attgtactga tatctaatcc ctggtgtctc attgtttata ctaggtatgg taaatgcagt 4021 atacttcctg aagtctttat ctaagggaac tgaaaaatat gcatcgccca catccagtac 4081 tgttactgat tttttctgtt ttaaccctgc aggatgtggt attcctaatt gaacttccca 4141 gaaatcttga gttctcttat taagttctct gaaatctact aattttctcc atttagtact 4201 gtcttttttc tttatggcaa atactggagt attgtatgga ttttcaggcc caatttttga 4261 aatttttcct tccttttcca tttctgtaca aatttctact aatgctttta ttttttcttc 4321 tgtcaatggc cattgtttaa cttttgggcc atccattcct ggctttaatt ttactggtac 4381 agtctcaata ggactaatgg gaaaatttaa agtgcagcca atctgagtca acagatttct 4441 tccaattatg ttgacaggtg taggtcctac taatactgta cctatagctt tatgtccgca 4501 gatttctatg agtatctgat catactgtct tactttgata aaacctccaa ttccccctat 4561 catttttggt ttccatcttc ctggcaaatt catttcttct aatactgtat catctgctcc 4621 tgtatctaat agagcttcct ttaattgccc ccctatcttt attgtgacga ggggtcgctg 4681 ccaaagagtg atctgaggga agctaaagga tacagttcct tgtctatcgg ctcctgcttc 4741 tgagagggag ttgttgtctc ttccccaaac ctgaagctct cttctggtgg ggctgttggc 4801 tctggtctgc tctgaagaaa attccctggc cttcccttgt gggaaggcca gatcttccct 4861 aaaaaattag cctgtctctc agtacaatct ttcatttggt gtccttcctt tccacatttc 4921 caacagccct ttttcctagg ggccctgcaa tttttggcta tgtgcccttc tttgccacaa 4981 ttgaaacact taacagtctt tctttggttc ctaaaattgc ctttctgtat cattatggta 5041 gctggatttg ttacttggct cattgcttca gccaaaactc ttgctttatg gccgggtccc 5101 cccactccct gacatgctgt catcatttct tctagtgtcg ctcctggtcc caatgctttt 5161 aaaatagtct tacaatctgg gttcgcattt tggaccaaca aggtttctgt catccaattt 5221 tttacctctt gtgaagcttg ctcggctctt agagttttat agaatcggtc tacatagtct 5281 ctaaagggtt cctttggtcc ttgtcttatg tccagaatgc tggtagggct atacattctt 5341 actattttat ttaatcccag gattatccat cttttataga tttctcctac tgggataggt 5401 ggattatgtg tcatccatcc tatttgttcc tgaagggtac tagtagttcc tgctatgtca 5461 cttccccttg gttctctcat ctggcctggt gcaataggcc ctgcatgcac tggatgcact 5521 ctatcccatt ctgcagcttc ctcattgatg gtctctttta acatttgcat ggctgcttga 5581 tgtcccccca ctgtgtttag catggtgttt aaatcttgtg gggtggctcc ttctgataat 5641 gctgaaaaca tgggtatcac ttctgggctg aaagccttct cttctactac ttttacccat 5701 gcatttaaag ttctaggtga tatggcctga tgtaccattt gcccctggat gttctgcact 5761 atagggtaat tttggctgac ctgattgctg tgtcctgtgt cagctgctgc ttgctgtgct 5821 tttttcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt 5881 atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt 5941 tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct 6001 gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact 6061 atatgtttta atttatattt tttctttccc cctggcctta accgaatttt ttcccatcga 6121 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tggcggcggc agatctcgaa 6181 ttcagatctc acgtgctttg ccaaagtgat gggccagcac acagaccagc acgttgccca 6241 ggagctgtgg gaggaagata agaggtatga acatgattag caaaagggcc tagcttggac 6301 tcagaataat ccagccttat cccaaccata aaataaaagc agaatggtag ctggattgta 6361 gctgctatta gcaatatgaa acctcttaca tcagttacaa tttatatgca gaaatattta 6421 tatgcagaaa tattgctatt gccttaaccc agaaattatc actgttattc tttagaatgg 6481 tgcaaagagg catgatacat tgtatcatta ttgccctgaa agaaagagat tagggaaagt 6541 attagaaata agataaacaa aaaagtatat taaaagaaga aagcattttt taaaattaca 6601 aatgcaaaat taccctgatt tggtcaatat gtgtaccctg ttacttctcc ccttcctatg 6661 acatgaactt aaccatagaa aagaagggga aagaaaacat caagggtccc atagactcac 6721 cctgaagttc tcaggatccg agctcggtac cacatgtaag cttcgagggg aggctggatc 6781 ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg gcgtctccag gcgatctgac 6841 ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta ccgcccattt 6901 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa 6961 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct 7021 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata 7081 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg 7141 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat 7201 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc 7261 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg 7321 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc 7381 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaac 7441 atggcggtaa tgttggacat gagccaatat aaatgtacat attatgatat ggatacaacg 7501 tatgcaatgg gccaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc 7561 ggatcctcta gcccatgggg gccccctcag gggatccacg tcaggtggca cttttcgggg 7621 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 7681 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 7741 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 7801 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 7861 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 7921 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 7981 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 8041 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 8101 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 8161 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 8221 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 8281 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 8341 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 8401 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 8461 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 8521 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 8581 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 8641 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 8701 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 8761 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 8821 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 8881 cttcagcaga //