LOCUS Exported 14008 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pcDNA3-myc-FLNa WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14008) AUTHORS Woo MS, Ohta Y, Rabinovitz I, Stossel TP, Blenis J TITLE Ribosomal S6 kinase (RSK) regulates phosphorylation of filamin A on an important regulatory site. JOURNAL Mol Cell Biol. 2004 Apr . 24(7):3025-35. PUBMED 15024089 REFERENCE 2 (bases 1 to 14008) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14008 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(11..28) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(182..770) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(262..281) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(941..1801) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1564..1583 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1802..1906) /gene="bla" /label=AmpR promoter primer_bind complement(1981..2000) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 2172..2551 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2552..2755 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2706..2726 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2800..2819 /label=T7 /note="T7 promoter, forward primer" promoter 2800..2818 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 2841..2870 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" polyA_signal 11080..11214 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(11357..11379) /label=pGEX 3' /note="pGEX vectors, reverse primer" promoter complement(11498..11516) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(11499..11516) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(11536..11553) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 11542..11766 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 11812..12240 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(11899..11918) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 12109..12130 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(12249..12269) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 12254..12583 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 12434..12569 /label=SV40 ori /note="SV40 origin of replication" primer_bind 12496..12515 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 12650..13444 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(12704..12723) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 13314..13333 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 13618..13739 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(13788..13804) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(13788..13804) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(13801..13823) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 13812..13828 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(13836..13866) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 13881..13902 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." ORIGIN 1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag 1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga 2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa 2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag 2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt 2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg 2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac 2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt 2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa 2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta 2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg 2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg 2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc 2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg 2761 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga 2821 gacccaagct tggtaccatg gagcagaagc tgatcagcga ggaggacctg gaattcagta 2881 gctcccactc tcgggcgggc cagagcgcag caggcgcggc tccgggcggc ggcgtcgaca 2941 cgcgggacgc cgagatgccg gccaccgaga aggacctggc ggaggacgcg ccatggaaga 3001 agatccagca gaacactttc acgcgctggt gcaacgagca cctgaagtgc gtgagcaagc 3061 gcatcgccaa cctgcagacg gacctgagcg acgggctgcg gcttatcgcg ctgttggagg 3121 tgctcagcca gaagaagatg caccgcaagc acaaccagcg gcccactttc cgccaaatgc 3181 agcttgagaa cgtgtcggtg gcactcgagt tcctggaccg cgagagcatc aaactggtgt 3241 ccatcgacag caaggccatc gtggacggga acctgaagct gatcctgggc ctcatctgga 3301 ccctgatcct gcactactcc atctccatgc ccatgtggga cgaggaggag gatgaggagg 3361 ccaagaagca gacccccaag cagaggctcc tgggctggat ccagaacaag ctgccgcagc 3421 tgcccatcac caacttcagc cgggactggc agagcggccg ggccctgggc gccctggtgg 3481 acagctgtgc cccgggcctg tgtcctgact gggactcttg ggacgccagc aagcccgtta 3541 ccaatgcgcg agaggccatg cagcaggcgg atgactggct gggcatcccc caggtgatca 3601 cccccgagga gattgtggac cccaacgtgg acgagcactc tgtcatgacc tacctgtccc 3661 agttccccaa ggccaagctg aagccagggg ctcccttgcg gcccaaactg aacccgaaga 3721 aagcccgtgc ctacgggcca ggcatcgagc ccacaggcaa catggtgaag aagcgggcag 3781 agttcactgt ggagaccaga agtgctggcc agggagaggt gctggtgtac gtggaggacc 3841 cggccggaca ccaggaggag gcaaaagtga ccgccaataa cgacaagaac cgcaccttct 3901 ccgtctggta cgtccccgag gtgacgggga ctcataaggt tactgtgctc tttgctggcc 3961 agcacatcgc caagagcccc ttcgaggtgt acgtggataa gtcacagggt gacgccagca 4021 aagtgacagc ccaaggtccc ggcctggagc ccagtggcaa catcgccaac aagaccacct 4081 actttgagat ctttacggca ggagctggca cgggcgaggt cgaggttgtg atccaggacc 4141 ccatgggaca gaagggcacg gtagagcctc agctggaggc ccggggcgac agcacatacc 4201 gctgcagcta ccagcccacc atggagggcg tccacaccgt gcacgtcacg tttgccggcg 4261 tgcccatccc tcgcagcccc tacactgtca ctgttggcca agcctgtaac ccgagtgcct 4321 gccgggcggt tggccggggc ctccagccca agggtgtgcg ggtgaaggag acagctgact 4381 tcaaggtgta cacaaagggc gctggcagtg gggagctgaa ggtcaccgtg aagggcccca 4441 agggagagga gcgcgtgaag cagaaggacc tgggggatgg cgtgtatggc ttcgagtatt 4501 accccatggt ccctggaacc tatatcgtca ccatcacgtg gggtggtcag aacatcgggc 4561 gcagtccctt cgaagtgaag gtgggcaccg agtgtggcaa tcagaaggta cgggcctggg 4621 gccctgggct ggagggcggc gtcgttggca agtcagcaga ctttgtggtg gaggctatcg 4681 gggacgacgt gggcacgctg ggcttctcgg tggaagggcc atcgcaggct aagatcgaat 4741 gtgacgacaa gggcgacggc tcctgtgatg tgcgctactg gccgcaggag gctggcgagt 4801 atgccgttca cgtgctgtgc aacagcgaag acatccgcct cagccccttc atggctgaca 4861 tccgtgacgc gccccaggac ttccacccag acagggtgaa ggcacgtggg cctggattgg 4921 agaagacagg tgtggccgtc aacaagccag cagagttcac agtggatgcc aagcacggtg 4981 gcaaggcccc acttcgggtc caagtccagg acaatgaagg ctgccctgtg gaggcgttgg 5041 tcaaggacaa cggcaatggc acttacagct gctcctacgt gcccaggaag ccggtgaagc 5101 acacagccat ggtgtcctgg ggaggcgtca gcatccccaa cagccccttc agggtgaatg 5161 tgggagctgg cagccacccc aacaaggtca aagtatacgg ccccggagta gccaagacag 5221 ggctcaaggc ccacgagccc acctacttca ctgtggactg cgccgaggct ggccaggggg 5281 acgtcagcat cggcatcaag tgtgcccctg gagtggtagg ccccgccgaa gctgacatcg 5341 acttcgacat catccgcaat gacaatgaca ccttcacggt caagtacacg ccccgggggg 5401 ctggcagcta caccattatg gtcctctttg ctgaccaggc cacgcccacc agccccatcc 5461 gagtcaaggt ggagccctct catgacgcca gtaaggtgaa ggccgagggc cctggcctca 5521 gtcgcactgg tgtcgagctt ggcaagccca cccacttcac agtaaatgcc aaagctgctg 5581 gcaaaggcaa gctggacgtc cagttctcag gactcaccaa gggggatgca gtgcgagatg 5641 tggacatcat cgaccaccat gacaacacct acacagtcaa gtacacgcct gtccagcagg 5701 gtccagtagg cgtcaatgtc acttatggag gggatcccat ccctaagagc cctttctcag 5761 tggcagtatc tccaagcctg gacctcagca agatcaaggt gtctggcctg ggagagaagg 5821 tggacgttgg caaagaccag gagttcacag tcaaatcaaa gggtgctggt ggtcaaggca 5881 aagtggcatc caagattgtg ggcccctcgg gtgcagcggt gccctgcaag gtggagccag 5941 gcctgggggc tgacaacagt gtggtgcgct tcctgccccg tgaggaaggg ccctatgagg 6001 tggaggtgac ctatgacggc gtgcccgtgc ctggcagccc ctttcctctg gaagctgtgg 6061 cccccaccaa gcctagcaag gtgaaggcgt ttgggccggg gctgcaggga ggcagtgcgg 6121 gctcccccgc ccgcttcacc atcgacacca agggcgccgg cacaggtggc ctgggcctga 6181 cggtggaggg cccctgtgag gcgcagctcg agtgcttgga caatggggat ggcacatgtt 6241 ccgtgtccta cgtgcccacc gagcccgggg actacaacat caacatcctc ttcgctgaca 6301 cccacatccc tggctcccca ttcaaggccc acgtggttcc ctgctttgac gcatccaaag 6361 tcaagtgctc aggccccggg ctggagcggg ccaccgctgg ggaggtgggc caattccaag 6421 tggactgctc gagcgcgggc agcgcggagc tgaccattga gatctgctcg gaggcggggc 6481 ttccggccga ggtgtacatc caggaccacg gtgatggcac gcacaccatt acctacattc 6541 ccctctgccc cggggcctac accgtcacca tcaagtacgg cggccagccc gtgcccaact 6601 tccccagcaa gctgcaggtg gaacctgcgg tggacacttc cggtgtccag tgctatgggc 6661 ctggtattga gggccagggt gtcttccgtg aggccaccac tgagttcagt gtggacgccc 6721 gggctctgac acagaccgga gggccgcacg tcaaggcccg tgtggccaac ccctcaggca 6781 acctgacgga gacctacgtt caggaccgtg gcgatggcat gtacaaagtg gagtacacgc 6841 cttacgagga gggactgcac tccgtggacg tgacctatga cggcagtccc gtgcccagca 6901 gccccttcca ggtgcccgtg accgagggct gcgacccctc ccgggtgcgt gtccacgggc 6961 caggcatcca aagtggcacc accaacaagc ccaacaagtt cactgtggag accaggggag 7021 ctggcacggg cggcctgggc ctggctgtag agggcccctc cgaggccaag atgtcctgca 7081 tggataacaa ggacggcagc tgctcggtcg agtacatccc ttatgaggct ggcacctaca 7141 gcctcaacgt cacctatggt ggccatcaag tgccaggcag tcctttcaag gtccctgtgc 7201 atgatgtgac agatgcgtcc aaggtcaagt gctctgggcc cggcctgagc ccaggcatgg 7261 ttcgtgccaa cctccctcag tccttccagg tggacacaag caaggctggt gtggccccat 7321 tgcaggtcaa agtgcaaggg cccaaaggcc tggtggagcc agtggacgtg gtagacaacg 7381 ctgatggcac ccagaccgtc aattatgtgc ccagccgaga agggccctac agcatctcag 7441 tactgtatgg agatgaagag gtaccccgga gccccttcaa ggtcaaggtg ctgcctactc 7501 atgatgccag caaggtgaag gccagtggcc ccgggctcaa caccactggc gtgcctgcca 7561 gcctgcccgt ggagttcacc atcgatgcaa aggacgccgg ggagggcctg ctggctgtcc 7621 agatcacgga tcccgaaggc aagccgaaga agacacacat ccaagacaac catgacggca 7681 cgtatacagt ggcctacgtg ccagacgtga caggtcgcta caccatcctc atcaagtacg 7741 gtggtgacga gatccccttc tccccgtacc gcgtgcgtgc cgtgcccacc ggggacgcca 7801 gcaagtgcac tgtcacaggt gctggcatcg gccccaccat tcagattggg gaggagacgg 7861 tgatcactgt ggacactaag gcggcaggca aaggcaaagt gacgtgcacc gtgtgcacgc 7921 ctgatggctc agaggtggat gtggacgtgg tggagaatga ggacggcact ttcgacatct 7981 tctacacggc cccccagccg ggcaaatacg tcatctgtgt gcgctttggt ggcgagcacg 8041 tgcccaacag ccccttccaa gtgacggctc tggctgggga ccagccctcg gtgcagcccc 8101 ctctacggtc tcagcagctg gccccacagt acacctacgc ccagggcggc cagcagactt 8161 gggccccgga gaggcccctg gtgggtgtca atgggctgga tgtgaccagc ctgaggccct 8221 ttgaccttgt catccccttc accatcaaga agggcgagat cacaggggag gttcggatgc 8281 cctcaggcaa ggtggcgcag cccaccatca ctgacaacaa agacggcacc gtgaccgtgc 8341 ggtatgcacc cagcgaggct ggcctgcacg agatggacat ccgctatgac aacatgcaca 8401 tcccaggaag ccccttgcag ttctatgtgg attacgtcaa ctgtggccat gtcactgcct 8461 atgggcctgg cctcacccat ggagtagtga acaagcctgc caccttcacc gtcaacacca 8521 aggatgcagg agaggggggc ctgtctctgg ccattgaggg cccgtccaaa gcagaaatca 8581 gctgcactga caaccaggat gggacatgca gcgtgtccta cctgcctgtg ctgccggggg 8641 actacagcat tctagtcaag tacaatgaac agcacgtccc aggcagcccc ttcactgccc 8701 gggtcacagg tgacgactcc atgcgtatgt cccacctaaa ggtcggctct gctgccgaca 8761 tccccatcaa catctcagag acggatctca gcctgctgac ggccactgtg gtcccgccct 8821 cgggccggga ggagccctgt ttgctgaagc ggctgcgtaa tggccacgtg gggatttcat 8881 tcgtgcccaa ggagacgggg gagcacctgg tgcatgtgaa gaaaaatggc cagcacgtgg 8941 ccagcagccc catcccggtg gtgatcagcc agtcggaaat tggggatgcc agtcgtgttc 9001 gggtctctgg tcagggcctt cacgaaggcc acacctttga gcctgcagag tttatcattg 9061 atacccgcga tgcaggctat ggtgggctca gcctgtccat tgagggcccc agcaaggtgg 9121 acatcaacac agaggacctg gaggacggga cgtgcagggt cacctactgc cccacagagc 9181 caggcaacta catcatcaac atcaagtttg ccgaccagca cgtgcctggc agccccttct 9241 ctgtgaaggt gacaggcgag ggccgggtga aagagagcat cacccgcagg cgtcgggctc 9301 cttcagtggc caacgttggt agtcattgtg acctcagcct gaaaatccct gaaattagca 9361 tccaggatat gacagcccag gtgaccagcc catcgggcaa gacccatgag gccgagatcg 9421 tggaagggga gaaccacacc tactgcatcc gctttgttcc cgctgagatg ggcacacaca 9481 cagtcagcgt caagtacaag ggccagcacg tgcctgggag ccccttccag ttcaccgtgg 9541 ggcccctagg ggaaggggga gcccacaagg tccgagctgg gggccctggc ctggagagag 9601 ctgaagctgg agtgccagcc gaattcagta tctggacccg ggaagctggt gctggaggcc 9661 tggccattgc tgtcgagggc cccagcaagg ctgagatctc ttttgaggac cgcaaggacg 9721 gctcctgtgg tgtggcttat gtggtccagg agccaggtga ctacgaagtc tcagtcaagt 9781 tcaacgagga acacattccc gacagcccct tcgtggtgcc tgtggcttct ccgtctggcg 9841 acgcccgccg cctcactgtt tctagccttc aggagtcagg gctaaaggtc aaccagccag 9901 cctcttttgc agtcagcctg aacggggcca agggggcgat cgatgccaag gtgcacagcc 9961 cctcaggagc cctggaggag tgctatgtca cagaaattga ccaagataag tatgctgtgc 10021 gcttcatccc tcgggagaat ggcgtttacc tgattgacgt caagttcaac ggtacccaca 10081 tccctggaag ccccttcaag atccgagttg gggagcctgg gcatggaggg gacccaggct 10141 tggtgtctgc ttacggagca ggtctggaag gcggtgtcac agggaaccca gctgagttcg 10201 tcgtgaacac gagcaatgcg ggagctggtg ccctgtcggt gaccattgac ggcccctcca 10261 aggtgaagat ggattgccag gagtgccctg agggctaccg cgtcacctat acccccatgg 10321 cacctggcag ctacctcatc tccatcaagt acggcggccc ctaccacatt gggggcagcc 10381 ccttcaaggc caaagtcaca ggcccccgtc tcgtcagcaa ccacagcctc cacgagacat 10441 catcagtgtt tgtagactct ctgaccaagg ccacctgtgc cccccagcat ggggccccgg 10501 gtcctgggcc tgctgacgcc agcaaggtgg tggccaaggg cctggggctg agcaaggcct 10561 acgtaggcca gaagagcagc ttcacagtag actgcagcaa agcaggcaac aacatgctgc 10621 tggtgggggt tcatggccca aggaccccct gcgaggagat cctggtgaag cacgtgggca 10681 gccggctcta cagcgtgtcc tacctgctca aggacaaggg ggagtacaca ctggtggtca 10741 aatgggggca cgagcacatc ccaggcagcc cctaccgcgt tgtggtgccc tgagtctggg 10801 gcccgtgcca gccggcagcc cccaagcctg ccccgctacc caagcagccc cgccctcttc 10861 ccctcaaccc cggcccaggc cgccctggcc gcccgcctgt cactgcagct gcccctgccc 10921 tgtgccgtgc tgcgctcacc tgcctcccca gccagccgct gacctctcgg ctttcacttg 10981 ggcagagggt agctgactag cacaatggat ctcgagggat cttccatacc taccagttct 11041 gcgcctgcag gtcgcggccg ctcgaggccg gcaaggccgg atccagacat gataagatac 11101 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa 11161 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac 11221 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc 11281 aagtaaaacc tctacaaatg tggtatggct gattatgatc cggctgcctc gcgcgtttcg 11341 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 11401 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 11461 ggggcgcagc catgaggtcg actctagagg gccctattct atagtgtcac ctaaatgcta 11521 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 11581 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 11641 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 11701 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 11761 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 11821 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 11881 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 11941 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 12001 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 12061 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 12121 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 12181 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 12241 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 12301 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 12361 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 12421 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 12481 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 12541 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 12601 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 12661 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 12721 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 12781 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 12841 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 12901 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 12961 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 13021 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 13081 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 13141 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 13201 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 13261 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 13321 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 13381 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 13441 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 13501 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 13561 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 13621 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 13681 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 13741 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 13801 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 13861 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 13921 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 13981 gggagaggcg gtttgcgtat tgggcgct //