LOCUS       Exported                 828 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Lentiviral vector with Tet-based inducible expression of mouse G 
            alpha 13 miR30-based shRNA, constitutive Venus fluorescent protein 
            coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    pSLIK-Venus-TmiR-G13
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     ncRNA           83..204
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           232..246
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           274..400
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(433..457)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga atctagtcga
       61 ctagggataa cagggtaatt gtttgaatga ggcttcagta ctttacagaa tcgttgcctg
      121 cacatcttgg aaacacttgc tgggattact tcttcaggtt aacccaacag aaggctcgag
      181 aaggtatatt gctgttgaca gtgagcgatg ggtgagtctg taaagtattt agtgaagcca
      241 cagatgtaaa tactttacag actcacccag tgcctactgc ctcggaattc aaggggctac
      301 tttaggagca attatcttgt ttactaaaac tgaatacctt gctatctctt tgatacattt
      361 ttacaaagct gaattaaaat ggtataaatt aaatcacttt tttcaattcg cggccgcact
      421 cgagatatct agacccagct ttcttgtaca aagtggttga taacatctcg agccataaag
      481 atggttaatt aacccaccca agatctggcc tccgcgccgg gttttggcgc ctcccgcggg
      541 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
      601 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
      661 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
      721 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
      781 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccggg
//
