LOCUS       Exported                 664 bp ds-DNA     linear   SYN 03-JUN-2021
DEFINITION  Entry vector with human U6 promoter driving both mouse G alpha 12 
            and G alpha 13 miR30-based shRNAs..
ACCESSION   .
VERSION     .
KEYWORDS    pEN_hU6miR-Gb2-K
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     ncRNA           80..201
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           229..243
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           271..397
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(430..529)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(641..660)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tatcttgtgg aaaggacgaa acaccgtgct cgcttcggca gcacatatac tagtcgacta
       61 gggataacag ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac
      121 atcttggaaa cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgagaag
      181 gtatattgct gttgacagtg agcgcgctca tgtattccca cgacaatagt gaagccacag
      241 atgtattgtc gtgggaatac atgagcatgc ctactgcctc ggaattcaag gggctacttt
      301 aggagcaatt atcttgttta ctaaaactga ataccttgct atctctttga tacattttta
      361 caaagctgaa ttaaaatggt ataaattaaa tcactttttt caattcgcgg ccgcactcga
      421 gatatctaga cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
      481 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
      541 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
      601 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
      661 attc
//
