LOCUS Exported 9754 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Destination donor vector for AAVS1 targeting (puromycin selection). ACCESSION . VERSION . KEYWORDS pAAVS1-Nst-CAG-DEST SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9754) AUTHORS Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K TITLE Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives. JOURNAL Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 10.1016/j.ymeth.2015.12.012. PUBMED 26707206 REFERENCE 2 (bases 1 to 9754) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9754 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 382..659 /label=chicken beta-actin promoter intron 660..1676 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1600..1622 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1684..1703 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" protein_bind 1770..1894 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1919..1949 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 2003..2662 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(2069..2088) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 3004..3309 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 3188..3207 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(3350..3474) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(3570..3589) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 3635..3690 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(3689..3708) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" misc_feature 4246..4775 /label=HA-R /note="right homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" primer_bind complement(4921..4940) /label=T7 /note="T7 promoter, forward primer" promoter complement(4922..4940) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4947..4964) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4947..4963) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4956..4978) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 5105..5560 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5192..5211) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5402..5423 /label=F1ori-F /note="F1 origin, forward primer" promoter 5586..5690 /gene="bla" /label=AmpR promoter CDS 5691..6551 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5909..5928) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6722..7310 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7211..7230 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7464..7481 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7598..7619 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7634..7664 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7672..7688 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7677..7699 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7696..7712 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7696..7712 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 7733..7751 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" misc_feature 7764..8567 /label=HA-L /note="left homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" misc_feature 8574..8599 /label=SA /note="splice acceptor site" CDS 8623..8676 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 8692..9486 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(8746..8765) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 9356..9375 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(9506..9523) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 9512..9736 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct 421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga 481 tgggggcggg gggggggggg gggcgcgcgc caggsggggc ggggcggggc grggggcggg 541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc 601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg 661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc 721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc 781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa 841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg 901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct 961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg 1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt 1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc 1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg 1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg 1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg 1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc 1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc 1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg 1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc 1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga 1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc 1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gcggccgcgg 1741 taccggcgcg ccggatcctg caggttatca caagtttgta caaaaaagct gaacgagaaa 1801 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat 1861 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt 1921 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt 1981 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat 2041 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta 2101 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca 2161 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt 2221 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac 2281 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt 2341 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta 2401 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt 2461 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat 2521 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca 2581 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga 2641 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta 2701 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag 2761 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc 2821 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc 2881 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg 2941 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt 3001 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat 3061 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca 3121 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa 3181 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa 3241 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg 3301 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg 3361 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat 3421 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgataac 3481 tctagagaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc 3541 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac 3601 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca 3661 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat 3721 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct 3781 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc 3841 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt 3901 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt 3961 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc 4021 gacctgcagc ccaagcttgg atccctcgag ttaattaaat ctagaagtcg acagtactaa 4081 gctttgacag aaaagcccca tccttaggcc tcctccttcc tagtctcctg atattgggtc 4141 taacccccac ctcctgttag gcagattcct tatctggtga cacaccccca tttcctggag 4201 ccatctctct ccttgccaga acctctaagg tttgcttacg atggagccag agaggatcct 4261 gggagggaga gcttggcagg gggtgggagg gaaggggggg atgcgtgacc tgcccggttc 4321 tcagtggcca ccctgcgcta ccctctccca gaacctgagc tgctctgacg cggctgtctg 4381 gtgcgtttca ctgatcctgg tgctgcagct tccttacact tcccaagagg agaagcagtt 4441 tggaaaaaca aaatcagaat aagttggtcc tgagttctaa ctttggctct tcacctttct 4501 agtccccaat ttatattgtt cctccgtgcg tcagttttac ctgtgagata aggccagtag 4561 ccagccccgt cctggcaggg ctgtggtgag gaggggggtg tccgtgtgga aaactccctt 4621 tgtgagaatg gtgcgtccta ggtgttcacc aggtcgtggc cgcctctact ccctttctct 4681 ttctccatcc ttctttcctt aaagagtccc cagtgctatc tgggacatat tcctccgccc 4741 agagcagggt cccgcttccc taaggccctg ctctgggctt ctgggtttga gtccttggca 4801 agcccaggag aggcgctcag gcttccctgt cccccttcct cgtccaccat ctcatgcccc 4861 tggctctcct gccccttccc tacaggggtt cctggctctg ctctagcgat cgccaattcg 4921 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa 4981 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 5041 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 5101 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 5161 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 5221 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 5281 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 5341 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 5401 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 5461 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 5521 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa 5581 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 5641 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 5701 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 5761 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 5821 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 5881 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 5941 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 6001 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 6061 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 6121 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 6181 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 6241 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 6301 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 6361 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 6421 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 6481 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 6541 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 6601 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 6661 cttaacgtga gttttcgttc cactgagcgt caaaccccgt agaaaagatc aaaggatctt 6721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 6781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 6841 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 6901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 6961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 7021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 7081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 7141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 7201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 7261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 7321 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 7381 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 7441 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 7501 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 7561 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 7621 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 7681 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc gaaattaacc 7741 ctcactaaag ggaacaaaag ctgtgctttc tctgaccagc attctctccc ctgggcctgt 7801 gccgctttct gtctgcagct tgtggcctgg gtcacctcta cggctggccc agatccttcc 7861 ctgccgcctc cttcaggttc cgtcttcctc cactccctct tccccttgct ctctgctgtg 7921 ttgctgccca aggatgctct ttccggagca cttccttctc ggcgctgcac cacgtgatgt 7981 cctctgagcg gatcctcccc gtgtctgggt cctctccggg catctctcct ccctcaccca 8041 accccatgcc gtcttcactc gctgggttcc cttttccttc tccttctggg gcctgtgcca 8101 tctctcgttt cttaggatgg ccttctccga cggatgtctc ccttgcgtcc cgcctcccct 8161 tcttgtaggc ctgcatcatc accgtttttc tggacaaccc caaagtaccc cgtctccctg 8221 gctttagcca cctctccatc ctcttgcttt ctttgcctgg acaccccgtt ctcctgtgga 8281 ttcgggtcac ctctcactcc tttcatttgg gcagctcccc tacccccctt acctctctag 8341 tctgtgctag ctcttccagc cccctgtcat ggcatcttcc aggggtccga gagctcagct 8401 agtcttcttc ctccaacccg ggcccctatg tccacttcag gacagcatgt ttgctgcctc 8461 cagggatcct gtgtccccga gctgggacca ccttatattc ccagggccgg ttaatgtggc 8521 tctggttctg ggtactttta tctgtcccct ccaccccaca gtggggcaag cttctgacct 8581 cttctcttcc tcccacaggg cctcgagaga tctggcagcg gagagggcag aggaagtctt 8641 ctaacatgcg gtgacgtgga ggagaatccc ggccctaggc tcgagggtac catgattgaa 8701 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 8761 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 8821 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag 8881 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 8941 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 9001 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 9061 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 9121 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 9181 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat 9241 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 9301 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 9361 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 9421 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 9481 ttctgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 9541 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 9601 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 9661 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 9721 gcggtgggct ctatggacta gcggtggcgg cctc //