LOCUS       Exported               10691 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    pBbdCas9S(RBSmut)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10691
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1435..1479
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1455..1471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1487..1503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1540..5646
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5816..6607
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6730..7318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7219..7238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgcgccaaa taatgcccgt gtccctgttg aatagggaca cggtttttta acaatttttt
       61 tattcctagg ccttagtggc cggtacccgg ggatccttat tactggccgc tttctagcct
      121 ggatacttgt ctggctagtt gcattagaga gtcagctaga gccctagggc tagctgtttg
      181 tgtattcgga gccagtgtag tttttctttt tactaacgat acgggtaata attgattgcc
      241 ttttacagcc tgaccttgag acaattgcaa aagtctgtcc acagaggttt gacctaataa
      301 tctaaaatct tgtttgatag tagtaagtgg aggtatgtag caactagaat cttctgtatc
      361 atcataaccc acaactgaaa tatcagcccc aactctaagt ccactttcag taatagctcg
      421 catagctcct aaagccattt gatcattagc aactaacata gcagtaggga caatgccttc
      481 atttaacatt tgcattgttt gctgaaatcc tgacatagca gaccaatctc cttctctttc
      541 tgctattggt tgtatttgat ttcttgttaa atacttgtgc catcctgcta atctcaatct
      601 ggcacttact gaacttaagg gacctgcaag tagtgcaatt tgttgatggc ctaatgcaac
      661 aaggtgttca actcctaacc ttgttccatc ttcgtgagaa aaaattatag agtttattgg
      721 ggtttggtcc gacacatcca aaaacaatgc tggaacatta gtacatgctg cttcaactgc
      781 aatcgcatct tggtcatcaa gaggataatt aattataagt ccactaacac gttgagctaa
      841 taaattatgt actgctgcct tacaagcctc tacacctgat ctctcaacca ttgatacaac
      901 aacgcttgca ccaagttgat cagctctcga tttaattgcg gctacaattt gactaggcgc
      961 atgtaatgca agtgatgatg tggcaactcc aattaaaagt gattgtttcc ccgcaagttg
     1021 ctgagcaacg cgatttggta tataattcaa ctctgccata gctgcttcaa ccttctctct
     1081 tgtttttgca gatacatgac ttgcttggtt tacaactctc gaaaccgttt ggtagctaac
     1141 cccggcatat tcagcaacat catacaaagt aacaggtttc atggatcatt cctccatgat
     1201 aaaatttaaa tttctgactt tggcaaatcc ttttgcctgt taaaaagaat agcacaaaaa
     1261 ttaaaaaaaa agaaacgcaa atagcaatag cattaaaaat aattattatt atttatttct
     1321 ttgattaaaa tcaaaagcct ttaatgtatt ttcttgatct ttcatgcgct taacggtaaa
     1381 atccaaggta ttaagttcac cttccacttt tctctctcta tcttctctag aaaatcataa
     1441 aaaatttatt tgctttgtga gcggataaca attataatag attcaattgt gagcggataa
     1501 caatttcaca cagaattcat taaagaagag aaattacata tggataagaa atactcaata
     1561 ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
     1621 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
     1681 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
     1741 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
     1801 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
     1861 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
     1921 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
     1981 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
     2041 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
     2101 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
     2161 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
     2221 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
     2281 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
     2341 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
     2401 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
     2461 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
     2521 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
     2581 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct
     2641 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
     2701 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
     2761 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa
     2821 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga
     2881 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
     2941 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca
     3001 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
     3061 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
     3121 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
     3181 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
     3241 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
     3301 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg
     3361 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
     3421 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
     3481 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
     3541 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
     3601 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
     3661 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
     3721 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
     3781 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
     3841 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
     3901 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
     3961 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
     4021 ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt
     4081 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
     4141 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
     4201 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt
     4261 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
     4321 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
     4381 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
     4441 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
     4501 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
     4561 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
     4621 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
     4681 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg
     4741 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
     4801 tccatgcccc aagtcaatat tgtcaagaaa acagaagtcc agacaggcgg attctccaag
     4861 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat
     4921 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
     4981 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
     5041 attctggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
     5101 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
     5161 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
     5221 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
     5281 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
     5341 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
     5401 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
     5461 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt
     5521 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt
     5581 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
     5641 gactaataaa agcttgatca gatctcagct tttttttgaa gtgcctggca gtaagttgaa
     5701 aaaatttgaa ataaacttgg actatgttaa taaaaaaata caaattaacg aacaaaaaga
     5761 tttgaattat tatataattt agttaaaagc aattttaaat gagggaggtt tccatatgag
     5821 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     5881 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     5941 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     6001 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     6061 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     6121 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     6181 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     6241 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     6301 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     6361 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     6421 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     6481 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     6541 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     6601 caaataatga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcatgac
     6661 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6721 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6781 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6841 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6901 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6961 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7021 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7081 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7141 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7201 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7261 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7321 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7381 ctttcctgca ctagatcccc ggcaagttca tccttagggt tttcatatct cttaaaaagt
     7441 caatttctgc ttgattaatt tcttgtggat tattgttttt gcggtttttc attttttatc
     7501 tccttgagta ttgaattttt tcttatatat aaatatatag caaaaactat ttttgccaac
     7561 ttttttacaa aaaatttttt attaaaacac ttagggcttt gctaaattct cttttaaaag
     7621 aacttagtaa agccctatgg atttaagaac tgattatact tacgtagtaa aaatactaca
     7681 gatattaata gtatagacca tatcaaaatc ataatcaatt tatatttatc taaaattgta
     7741 gtccatacaa aatacaaaaa aattaggttt ttctaaattc tattttaaaa gaacttagta
     7801 acacctaatc tctaaattac ttacaaaatt ctaaaattag atttttagtt cttcatattt
     7861 ctttaaaagt tttttaagaa aatctttttg attttcaaaa atttcatgca tcataaaact
     7921 agtaaatttg gatttttttt tgtaaaaatc ataactttct tgggttttaa gttgaaatct
     7981 taatggtttt attggatttt gcctagattt ttttaacgcc ggactttctt tatcttttat
     8041 aaaattataa gaatttttaa taccattttc tattaaataa tcttctttta taagcccctc
     8101 ttgcagtgca gttgctaatc ttaaatactt atacgcttga gtttttgcta catcaaagtc
     8161 ttttataaaa tcaagaaaag ttttataacc atcaaattta taatattgat tatcttttat
     8221 ttcttttaaa attttaatat tttccaccct gtggaatatt tcttttttga aattgtcatt
     8281 taacttttcc tttagtgtct tgtaataaga aattttactt tcattaatca ttaaattttg
     8341 ttcaacataa gtatctatct tttttgttaa aactttaaca acacgttttt tttccataaa
     8401 aattttcctt tatttttgca acattttcct taattgatat aattgttact attttagatt
     8461 ttttaatatc cacttaagaa ttaatccctc actagaatat tttttattat agaattcata
     8521 tacatgtttt tctaaatcta taaatttatc aacaattgct ttattatagg gtgtatctat
     8581 cttttctttt cttaataatc gtctcatatt ctttaaataa caacaaacac tgccggtttt
     8641 aaacctaaat tctatgtaat atatttttga aaaagtatat acattttcaa ccccgtttat
     8701 ttcatacttt acaaaaacat tatttattgg ttttctataa ccatagtaaa tgcctaaaaa
     8761 tttgtccccg tctttcatag gatatagata aagtttttga atctctgatt tatctaataa
     8821 tttttcaaaa aaaattatac accgcttttg tttgttacta actactctat gcaatgcctt
     8881 taatattttt gtgtgatata tatttttatc accttttttt tctttcagca ggaagatact
     8941 ttcttttgga aatttaattt tttgttcatt tattcttgca gccttaagtc cgattttcat
     9001 atttaatcct tatatagcct ttctatttaa ttcttttaaa ctcaataaac ttttttggtt
     9061 tttcattagt tctaataatt cgcaataata tgtgcaaaac actttattgt attctatttt
     9121 cttttggtta ttcaaatatt cttttataat tggctttaga agttcaatat ttacttcttt
     9181 ttttagttgt tcaataagga tattgaaaat gtttgttttt aaatcttgat aattttgttg
     9241 agaattttct ttttttcttt caattgactt ctctaattta cgctttatat tgtttaaatc
     9301 gctatattta tgactttcaa taataaaatg aggcttaaat ttgtaatttt cgtatatttt
     9361 ttggaaactt gtttctaatt gtttagaatt atattcattt ttttcaaatt ctttttgagt
     9421 gttgcataga atttccttta atttttcttg tttttctttc atacaagatt tagtaaaatg
     9481 tatattttta ttttttagta atttaatttc tattattttt atgattttaa gtattttgat
     9541 tatattatct ttatcaatat ttaaattgaa taaaattgta agaaaaattg aaagaatttt
     9601 tttacataaa aagttacatt tattgaagta atttcttact tgatactttt ctatttcatt
     9661 aatctttctt tcttctttta tattattttt attatttaaa cactccttta aatctacact
     9721 cccattttta gtaaatctat ctttaaggcc ccttgaaact ctattttgaa atcttaaatc
     9781 ttttttttct tgaaagtatt tatagatttt ttggtaacat tctttttttg aaaaattaag
     9841 ttgatagtat atttcggtcc ccaaatttac ccctaagtgt ttgtgataat tagttgtgac
     9901 tttaaattct ttttctaatt tgtaaaggta attttgcagt gtttttagtt taacgggtgt
     9961 ttgactattt cttttaaggt tttcattaaa gtaatagagt atgttttttt gtgtgtattt
    10021 cttatatgtt ttatttatgt acgctagtgt tgagataagg actactaatt tatactgtaa
    10081 tttattgtag caagttgtgg ttttactggt tttgtttata ttagataagt tttccattta
    10141 tagctccttg aataccggat tttttatcta taaagtatat atatcacaat tttgatttaa
    10201 aagtaaattc tttttttaaa aatacaaaaa ttttttataa attatataga ccatgatatt
    10261 gtattaatac taattgattt taatttaagg ttgaactaaa ataaacatag ttttgtagaa
    10321 aataattttt cattattttg ctacttgata ttggatcgta aaaatattag ggctttacaa
    10381 agttctttta aaagagaatt tagtaaagcc ctaagtcttt taataaaaaa ttttttgtaa
    10441 aaaagttggc aaaaatagtt tttgctatat atttatatat aagaaaaaat tcaatactca
    10501 aggagataaa aaatggaaaa cttaaacaat aatccacaaa aaattaatca aataaataag
    10561 caaactaatt ttattggttt tgaaaatttt atagaacttg ataatcaagt taatcaaact
    10621 aaagaaaaaa gtaaattaag taagataggt aagaaattac ccggaataag cagtcaagtc
    10681 tagtgcggcc g
//
