LOCUS       Exported                5540 bp ds-DNA     circular SYN 08-JAN-2024
DEFINITION  AAV-mediated expression of ultrafast protein calcium sensor under 
            the Syn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pGP-AAV-syn-jGCaMP8s-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Zhang , Yan; Rozsa, Marton; Bushey, Daniel; Zheng, Jihong; Reep, 
            Daniel; Broussard, Gerard Joey; Tsang, Arthur; Tsegaye, Getahun; 
            Patel, Ronak; Narayan, Sujatha; Li, Jing Xuan; Zhang, Rongwei; 
            Ahrens, Misha B.; Turner, Glenn C.; Wang, Samuel S.-H.; Svoboda, 
            Karel; Korff, Wyatt; Schreiter, Eric R.; Hasseman, Jeremy P.; Kolb, 
            Ilya; Looger, Loren L
  TITLE     jGCaMP8 Fast Genetically Encoded Calcium Indicators
  JOURNAL   Janelia Research Campus. Online resource. 2020
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 8, 2024 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   95..224
                     /label=AAV2 ITR
     promoter        306..753
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             794..811
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     1093..1114
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1220..1241)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1481..1500)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2072..2660
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2125..2145)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2543..2554)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2663..2679)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2664..2680)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2709..2830
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2720..2739)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2774..2793
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2992..3121
                     /label=AAV2 ITR
     primer_bind     complement(3187..3204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3358..3946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3438..3457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4117..4977)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4740..4759
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4978..5082)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5108..23)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5245..5266)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5457..5476
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
       61 gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc
      121 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
      181 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa
      241 cccgccatgc tacttatcta cgtagccatg ctctaggaag atctctgcag agggccctgc
      301 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      361 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      421 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      481 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      541 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      601 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      661 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      721 agcggaggag tcgtgtcgtg cctgagagcg cagtcgaatt caagctgcta gcaaggatcc
      781 acccgccacc atgcatcatc accatcatca cacgcgtcgc aagaagacct tcaaggaggt
      841 ggccaccgcc gtgaagatca tcgccatgct gatgggcctg aagatcaacg tctatatcaa
      901 ggccgacaag cagaagaacg gcatcaaggc gaacttccac atccgccaca acatcgagga
      961 cggcggcgtg cagctcgcct accactacca gcagaacacc cccatcggcg acggccccgt
     1021 gctgctgccc gacaaccact acctgagcgt ggagtccaaa ctttcgaaag accccaacga
     1081 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1141 ggacgagctg tacaagggcg gtaccggagg gagcatggtg agcaagggcg aggagctgtt
     1201 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1261 cgtgtccggc gagggtgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1321 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     1381 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1441 gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1501 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1561 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacc tgccggacca
     1621 actgactgaa gagcagatcg cagaatacaa agaggctttc tccctatttg acaaggacgg
     1681 ggatgggaca ataacaacca aggagctggg gacggtgatg cggtctctcg gacacaaccc
     1741 cacagaagca gagctgcagg acatgatcaa tgaagtagat gccgacggtg acggcacaat
     1801 cgacttccct gagttcctga caatgatggc aagaaaaatg aaatacaggg acacggaaga
     1861 agaaattaga gaagcgttcg gtgtgtttga taaggatggc aatggctaca tcagtgcagc
     1921 agagcttcgc cacgtgatga caaaccttgg agagaagtta acagatgaag aggttgatga
     1981 aatgatcagg gaagcagaca tcgatgggga tggtcaggta aactacgaag agtttgtaca
     2041 aatgatgaca gcgaagtaaa agcttatcga taatcaacct ctggattaca aaatttgtga
     2101 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     2161 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     2221 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     2281 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     2341 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     2401 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     2461 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     2521 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2581 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     2641 cctttgggcc gcctccccgc atcgataccg tcgacctcga cccgggcggc cgcttcgagc
     2701 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2761 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2821 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     2881 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatcttcc
     2941 tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac aaggaacccc
     3001 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     3061 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     3121 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4081 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4141 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4201 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4261 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4321 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4381 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4441 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4501 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4561 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4621 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4681 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4741 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4801 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4861 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4921 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4981 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5041 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5101 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     5161 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     5221 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     5281 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     5341 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     5401 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     5461 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     5521 accaccacac ccgccgcgct
//
