LOCUS Exported 5865 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Nucleus/Histones, Excitation: 553, Emission: 576. ACCESSION . VERSION . KEYWORDS tdTurboRFP-H2B-6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5865) TITLE Davidson Orange Fluorescent Proteins REFERENCE 2 (bases 1 to 5865) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5865 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 657..1034 /codon_start=1 /gene="HIST1H2BJ" /product="human histone H2B" /label=H2B /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP GELAKHAVSEGTKAITKYTSAK" CDS 1053..1745 /codon_start=1 /product="red fluorescent protein from Entacmaea quadricolor" /label=TurboRFP /note="mammalian codon-optimized" /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC DLPSKLGHR" CDS 1815..2507 /codon_start=1 /product="red fluorescent protein from Entacmaea quadricolor" /label=TurboRFP /note="mammalian codon-optimized" /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC DLPSKLGHR" polyA_signal 2653..2774 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2690..2709) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2744..2763 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2781..3236) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2918..2939) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3130..3149 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3263..3367 /gene="bla" /label=AmpR promoter promoter 3369..3726 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3392..3412) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3577..3712 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3639..3658 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3761..4555 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3815..3834) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4425..4444 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4743..4762) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4787..4834 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5163..5751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5652..5671 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gccaccatgc 661 cagagccagc gaagtctgct cccgccccga aaaagggctc caagaaggcg gtgactaagg 721 cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa ggagagctat tccatctatg 781 tgtacaaggt tctgaagcag gtccaccctg acaccggcat ttcgtccaag gccatgggca 841 tcatgaattc gtttgtgaac gacattttcg agcgcatcgc aggtgaggct tcccgcctgg 901 cgcattacaa caagcgctcg accatcacct ccagggagat ccagacggcc gtgcgcctgc 961 tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg tactaaggcc atcaccaagt 1021 acaccagcgc taaggatcca ccggtcgcca ccatgagcga gctgatcaag gagaacatgc 1081 acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg 1141 gcgaaggcaa gccctacgag ggcacccaga ccatgaagat caaggtggtc gagggcggcc 1201 ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc aaagccttca 1261 tcaaccacac ccagggcatc cccgacttct ttaagcagtc cttccctgag ggcttcacat 1321 gggagagaat caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagct 1381 tccagaacgg ctgcatcatc tacaacgtca agatcaacgg ggtgaacttc ccatccaacg 1441 gccctgtgat gcagaagaaa acacgcggct gggaggccaa caccgagatg ctgtaccccg 1501 ctgacggcgg cctgagaggc cacagccaga tggccctgaa gctcgtgggc gggggctacc 1561 tgcactgctc cttcaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc 1621 ccggcttcca cttcgtggac cacagactgg aaagaatcaa ggaggccgac aaagagacct 1681 acgtcgagca gcacgagatg gctgtggcca agtactgcga cctccctagc aaactggggc 1741 acagagggca tggcaccggc agcaccggca gcggcagctc cggtaccgcc tcctccgagg 1801 acaacaacat ggccatgagc gagctgatca aggagaacat gcacatgaag ctgtacatgg 1861 agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg 1921 agggcaccca gaccatgaag atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg 1981 acatcctggc taccagcttc atgtacggca gcaaagcctt catcaaccac acccagggca 2041 tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga atcaccacat 2101 acgaagacgg gggcgtgctg accgctaccc aggacaccag cttccagaac ggctgcatca 2161 tctacaacgt caagatcaac ggggtgaact tcccatccaa cggccctgtg atgcagaaga 2221 aaacacgcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc ggcctgagag 2281 gccacagcca gatggccctg aagctcgtgg gcgggggcta cctgcactgc tccttcaaga 2341 ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcttc cacttcgtgg 2401 accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgaga 2461 tggctgtggc caagtactgc gacctcccta gcaaactggg gcacagagat gaatccggac 2521 tcagatcttg agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt 2581 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca 2641 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 2701 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 2761 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa 2821 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2881 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2941 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 3001 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 3061 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3121 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3181 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg 3241 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 3301 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 3361 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag 3421 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 3481 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 3541 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 3601 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 3661 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 3721 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 3781 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 3841 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 3901 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct 3961 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 4021 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 4081 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 4141 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 4201 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 4261 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac 4321 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 4381 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 4441 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 4501 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 4561 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 4621 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 4681 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta 4741 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga 4801 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc 4861 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc 4921 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg 4981 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt 5041 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 5101 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 5161 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 5221 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 5281 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 5341 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 5401 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 5461 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 5521 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 5581 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 5641 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 5701 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 5761 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 5821 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat //