LOCUS       Exported                9056 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses IL6 receptor (full length) in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    IL6R PLAK1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Khatib A, Solaimuthu B, Ben Yosef M, Abu Rmaileh A, Tanna M, Oren G,
            Schlesinger Frisch M, Axelrod JH, Lichtenstein M, Shaul YD
  TITLE     The glutathione peroxidase 8 (GPX8)/IL-6/STAT3 axis is essential in 
            maintaining an aggressive breast cancer phenotype.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Sep 1;117(35):21420-21431. doi: 
            10.1073/pnas.2010275117. Epub 2020 Aug 18.
  PUBMED    32817494
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1833..1853
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2142..2345
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2296..2316
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3822..3887
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    3935..4052
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4101..4611
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4530..4548
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     LTR             5575..5808
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5880..6001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5917..5936)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5971..5990
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6041..6176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6103..6122
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6196..6215)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6197..6215)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6225..6242)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6225..6241)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6234..6256)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6383..6838
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6470..6489)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6680..6701
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6864..6968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6969..7829
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7187..7206)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8000..8588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8489..8508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8742..8759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8876..8897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8912..8942
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8950..8966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8955..8977
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8974..8990
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8974..8990
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9009..9029
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9011..9029
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcagca gcccccttca ccgagggcct atttcccatg attccttcat
     1861 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     1921 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     1981 ttttaaaatt atgttttaaa atggactatc atatgccaag tacgccccct attgacgtca
     2041 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2101 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2161 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2221 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     2281 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2341 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtttaattaa tccaacatgc
     2401 tggccgtcgg ctgcgcgctg ctggctgccc tgctggccgc gccgggagcg gcgctggccc
     2461 caaggcgctg ccctgcgcag gaggtggcga gaggcgtgct gaccagtctg ccaggagaca
     2521 gcgtgactct gacctgcccg ggggtagagc cggaagacaa tgccactgtt cactgggtgc
     2581 tcaggaagcc ggctgcaggc tcccacccca gcagatgggc tggcatggga aggaggctgc
     2641 tgctgaggtc ggtgcagctc cacgactctg gaaactattc atgctaccgg gccggccgcc
     2701 cagctgggac tgtgcacttg ctggtggatg ttccccccga ggagccccag ctctcctgct
     2761 tccggaagag ccccctcagc aatgttgttt gtgagtgggg tcctcggagc accccatccc
     2821 tgacgacaaa ggctgtgctc ttggtgagga agtttcagaa cagtccggcc gaagacttcc
     2881 aggagccgtg ccagtattcc caggagtccc agaagttctc ctgccagtta gcagtcccgg
     2941 agggagacag ctctttctac atagtgtcca tgtgcgtcgc cagtagtgtc gggagcaagt
     3001 tcagcaaaac tcaaaccttt cagggttgtg gaatcttgca gcctgatccg cctgccaaca
     3061 tcacagtcac tgccgtggcc agaaaccccc gctggctcag tgtcacctgg caagaccccc
     3121 actcctggaa ctcatctttc tacagactac ggtttgagct cagatatcgg gctgaacggt
     3181 caaagacatt cacaacatgg atggtcaagg acctccagca tcactgtgtc atccacgacg
     3241 cctggagcgg cctgaggcac gtggtgcagc ttcgtgccca ggaggagttc gggcaaggcg
     3301 agtggagcga gtggagcccg gaggccatgg gcacgccttg gacagaatcc aggagtcctc
     3361 cagctgagaa cgaggtgtcc acccccatgc aggcacttac tactaataaa gacgatgata
     3421 atattctctt cagagattct gcaaatgcga caagcctccc agtgcaagat tcttcttcag
     3481 taccactgcc cacattcctg gttgctggag ggagcctggc cttcggaacg ctcctctgca
     3541 ttgccattgt tctgaggttc aagaagacgt ggaagctgcg ggctctgaag gaaggcaaga
     3601 caagcatgca tccgccgtac tctttggggc agctggtccc ggagaggcct cgacccaccc
     3661 cagtgcttgt tcctctcatc tccccaccgg tgtcccccag cagcctgggg tctgacaata
     3721 cctcgagcca caaccgacca gatgccaggg acccacggag cccttatgac atcagcaata
     3781 cagactactt cttccccaga ggaggcagcg cggccgcagg cgactacaaa gaccatgacg
     3841 gtgattataa agatcatgac attgattaca aggatgacga tgacaagtaa taagaattct
     3901 cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     3961 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4021 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     4081 cactttggcc gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
     4141 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     4201 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     4261 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     4321 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
     4381 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     4441 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     4501 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     4561 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca
     4621 ccggagctta ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     4681 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     4741 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     4801 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     4861 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     4921 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     4981 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     5041 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     5101 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     5161 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     5221 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     5281 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     5341 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     5401 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacgc ccgccccacg
     5461 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggtacct ttaagaccaa
     5521 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5581 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     5641 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5701 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5761 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     5821 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     5881 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5941 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6001 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     6061 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6121 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6181 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     6241 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     6301 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     6361 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6421 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6481 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6541 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6601 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6661 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6721 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6781 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     6841 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6901 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6961 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7021 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7081 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7141 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7201 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7261 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7321 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7381 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7441 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7501 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7561 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7621 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7681 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7741 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7801 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7861 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7921 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7981 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8041 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8101 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8161 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8221 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8281 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8341 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8401 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8461 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8521 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8581 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8641 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8701 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8761 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8821 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     8881 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     8941 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     9001 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaa
//
