LOCUS Exported 13248 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Express mouse p150 protein with PIP2 deletion mutation. ACCESSION . VERSION . KEYWORDS pWPXL-p150-PIP2-Delta SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13248) AUTHORS Cheng L, Zhang X, Wang Y, Gan H, Xu X, Lv X, Hua X, Que J, Ordog T, Zhang Z TITLE Chromatin Assembly Factor 1 (CAF-1) facilitates the establishment of facultative heterochromatin during pluripotency exit. JOURNAL Nucleic Acids Res. 2019 Dec 2;47(21):11114-11131. doi: 10.1093/nar/gkz858. PUBMED 31586391 REFERENCE 2 (bases 1 to 13248) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13248 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 2..635 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 682..807 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1300..1533 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1718..1762 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 2145..3323 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2376..3314 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3271..3291 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" misc_feature 3371..3488 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 5309..5320 /label=ZF binding site /bound_moiety="CCR5TC zinc-finger domain" /note="target sequence for the CCR5TC zinc-finger domain from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 2008; Gross et al., 2013)" regulatory 6275..6284 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6281..6997 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6281..6997 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6326..6347) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6587..6606) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6934..6955 /label=EGFP-C /note="EGFP, forward primer" misc_feature 7079..7667 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7132..7152) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7550..7561) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" protein_bind 7811..7844 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 7864..8044 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" promoter complement(8069..8087) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(8070..8087) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(8377..8396) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(8610..8629) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8729..8751 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(8789..8807) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8875..8979 /gene="bla" /label=AmpR promoter CDS 8980..9840 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9198..9217) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10011..10599 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10500..10519 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10753..10770 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(10840..10860) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 10845..11174 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 11025..11160 /label=SV40 ori /note="SV40 origin of replication" primer_bind 11087..11106 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 12308..12373 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 12503..12523 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 12948..13082 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(12985..13004) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 13039..13058 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac 61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac 1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga 1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa 1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt 1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag 1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca 1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc 1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact 1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat 1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat 1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga 1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat 1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact 1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc 1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag 1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg 2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc 2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc 2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt 2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc 2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg 2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg 2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc 2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt 2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc 2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag 2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa 2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg 2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa 2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt 2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa 2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag 3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt 3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt 3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg 3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg 3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt 3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt 3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag 3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc 3481 aaaattttat cgatcacgag actagcctcg aggtttaaac tacgggatcc cgatgttgga 3541 ggagccggag gccgcgacgc ggacagccgc ggccgtggat tgcaaagaca gacccgggtt 3601 cccagtgaag aggttaatac aagcccgctt gcccttcaaa cgcctgaacc ttgttcccaa 3661 ggagaaagtt gaggaagaca catcaccaaa agcagctgtg gaaagcaaag tcccggatct 3721 acaattgtct ttgggcactt ttgaaagcca gtgtcacaca ggctctcacg taggcttgag 3781 tacaaagttg gtcggtggcc agggccccat tgatagcttc ttgagggcaa caatcaagcc 3841 agtaccgagt gtggtcatta tcgacctgac ggagaactgc agtgacattc cagacagccc 3901 tgagggccac agtgaactaa gtcctgacac tgctggtgtg gtcaccacag tggaaggagc 3961 tgccaagcag caggagcact ctgcagcaga gctgtgtctc ctagagaccc cttcagacat 4021 cacttgtcac atggaagagg agcctgggag tccaggagac ccaaagagga cgggtgactg 4081 tcaggctggt tcactacaga gctgtccgga gctgacccca ggttctagga catgccccac 4141 caaggagtta tcaagctgga gcaaggcagg ggatctccta tttatagaga aagtgcctgt 4201 ggtggtgcta gaggacatcc tggccactaa gccctccatt gcctctcttc ccatgatgtc 4261 cctggatagg agtgtcactt cagagagtga aatactggag tcctgccccg aggatgactc 4321 catactgagc cactcctcca ccaactcctc ttctccaacc agctctcctg agggaccatc 4381 cacaccccca gaacatcggg gtgggagaag ctccccctcc accccagcct gcagagttgc 4441 caagaacttt gtcaaaggct ccacagagaa gggcaggagc aaactgcaca gagatagaga 4501 acagcagagg gaagagaagg aaaagctgag ggaagagata cgacgagcca aggaggaggc 4561 acggaagaag aaggaggagg agaaggagct gaaggaaaag gagcggaggg aaaagcggga 4621 gaaagatgag aaggagaagg cggagaagca gcggctaaag gaggaaaaac gcaaggagcg 4681 gcaggaagct ctggaggcta aactggagga gaagaggaag aaggaggaag agaagcggtt 4741 acgggaggaa gagaagcggt tacgggaaga agagaagcgc attaaggcag aaaaggcaga 4801 gcagaaaccg aagactccgc aggctcccaa gaccctggcc ggttcctgtg gtaagtttgc 4861 cccctttgaa atcaaagaac acatggtgct ggctccacgg tgccgagctg ccttggatca 4921 agacctgtgc gatcagctgg accagctcct gcagcagcag agtgtggcaa gcaccttcct 4981 cagtgaccta aaaagccggc tgcccttgag atcggggccc acccgggtct gtggccatga 5041 cacagacatc atgaacaggg atgtggtcat tgtggagagc agcaaggtgg acggtgtgtc 5101 tgaaaggaag aagtttggcc gcatgaagct tctgcagttc tcagagaacc accggccagc 5161 atactggggg acgtggaaca agaagacagc catcatccgc ccccggaacc cctgggccca 5221 ggacaaagac cttcttgact atgaggtaga cagtgacgat gagtgggaag aggaggaacc 5281 tggggagtct ttgtcccaca gtgaggggga tgaggatgac gatgtggggg aagatgagga 5341 tgaggacgat ggcttctttg tgccccatgg gtacttatct gaagatgaag gcgtgactga 5401 ggagtgtgca gaccctgaga accacaaagt acaccagaag ctgaaggcca aggagtggga 5461 tgagctccta gccaagggca agcgctttcg tgtgctgcag cctgtgcacg tgggatgtgt 5521 atgggcatca gaggcagcca actgcacgag ttccgacttg aagctgctgc agcagttcac 5581 tgcatgcctg ctggatgtgg catcccctga tgagccagaa cccggggcct ccaggaggga 5641 aaagagggac cagcatatcc tggcccagtt gcttccgctg ctacatggca atgtgaatgg 5701 gagcaaggtg atcatccatg aattccagga gcaatgccgc aggggcctgc tgacccttcc 5761 aagtcccacc ccacacctgc agatgcccaa cttggaggat gctgttgctg tgccatccaa 5821 ggccaggctg aagcgcctta tctctgagaa ctctgcctat gagaagcggc ccaacttccg 5881 tatgtgctgg tatgtgcacc cagaggtgct caagagcttt ggccaggagt gcctgccagt 5941 gccctgccag tggacctaca tcaccaccat gccctcagca cccagggaag acagtggcag 6001 tgcttctact gaggggcctg gccagagcac acctatgcta ctcaagcgga agccagcggc 6061 caccatgtgc atcacgcagt ttatgaagaa gcggcgctat gatggacagg ttgggtctgg 6121 ggacatggat ggcttccagg cagacacaga agaggatgaa gaggatgaca cagactgtat 6181 gatcatagat gtgccagatg ttgggagtga cgtgtcagaa gctcccatac ctgctcccac 6241 actttgcaag gcacgcgtcc tagcgctacc ggtcgccacc atggtgagca agggcgagga 6301 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 6361 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 6421 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 6481 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 6541 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 6601 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 6661 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 6721 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 6781 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 6841 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 6901 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 6961 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgactagc 7021 tagtagctag ctagctagtc gagctcaagc ttcgaattcc cgggactagt catatgataa 7081 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 7141 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 7201 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 7261 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 7321 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 7381 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 7441 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 7501 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 7561 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 7621 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc ggtacgtatg 7681 gccaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 7741 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat gggatcaatt 7801 caccatggga ataacttcgt atagcataca ttatacgaag ttatgctgct ttttgcttgt 7861 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 7921 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 7981 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 8041 agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga 8101 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt 8161 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag 8221 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg 8281 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg 8341 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg 8401 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg 8461 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg 8521 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata 8581 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc 8641 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 8701 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 8761 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat 8821 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 8881 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 8941 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 9001 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 9061 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 9121 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 9181 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 9241 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 9301 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 9361 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 9421 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 9481 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 9541 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 9601 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 9661 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 9721 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 9781 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 9841 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 9901 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 9961 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 10021 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 10081 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 10141 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 10201 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 10261 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 10321 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 10381 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 10441 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 10501 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 10561 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 10621 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 10681 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 10741 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 10801 cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt 10861 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 10921 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 10981 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 11041 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 11101 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 11161 taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca 11221 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg 11281 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt 11341 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg 11401 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg 11461 aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac 11521 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg 11581 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc 11641 tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg 11701 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc 11761 cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct 11821 ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc 11881 gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg 11941 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc 12001 tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac 12061 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc 12121 ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac 12181 gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt 12241 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag 12301 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt 12361 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt 12421 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc 12481 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt 12541 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg 12601 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa 12661 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct 12721 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt 12781 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag 12841 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 12901 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 12961 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 13021 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 13081 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta 13141 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt 13201 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtag //