LOCUS       Exported                8815 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  GoldenBraid level alpha luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    pEPYC-alpha-1KN0003
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    55..88
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    114..147
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    173..206
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    232..265
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    295..328
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    354..387
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        404..450
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             526..2175
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(592..610)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             2239..2262
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      2285..2485
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    2506..2530
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3831..4460
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4889..5962
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6028..6222
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6458..6480)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6566..6706
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6621..6640
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6721..6738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6892..7480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6972..6991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7568..8383)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8291..8310
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tggcaggata tattgtggtg taaacataac gaattcgtct caggagcggt gcgggtgcca
       61 gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cgagccggtg cgggtgccag
      121 ggcgtgccct tgggctcccc gggcgcgtac tccactcccc tgaccggtgc gggtgccagg
      181 gcgtgccctt gggctccccg ggcgcgtact ccactccccg agccggtgcg ggtgccaggg
      241 cgtgcccttg ggctccccgg gcgcgtactc cactcccccc atggtgcggt gcgggtgcca
      301 gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cgagccggtg cgggtgccag
      361 ggcgtgccct tgggctcccc gggcgcgtac tccactcccc ttcgcaagac ccttcctcta
      421 tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa ttaccacaac
      481 aaaacaaaca acaaacaaca ttacaattta ctattctagt cgaaaatgga ggacgccaaa
      541 aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa
      601 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
      661 catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa
      721 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct
      781 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg
      841 aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg
      901 gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc
      961 atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac
     1021 acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc
     1081 ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct
     1141 aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct
     1201 atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac
     1261 ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg
     1321 tatagatttg aagaagaact gtttctgagg agccttcagg attacaagat tcaaagtgcg
     1381 ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat
     1441 ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa
     1501 gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact
     1561 acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt
     1621 gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat
     1681 caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg
     1741 gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac
     1801 tgggacgagg acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa
     1861 ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc
     1921 gacgcaggtg tcgcaggtct gcccgacgat gacgccggtg aacttcccgc cgccgttgtt
     1981 gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa
     2041 gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt
     2101 cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc
     2161 ggaaagatcg ccgtgggttc ggattataag gaccatgacg gagactataa ggaccatgac
     2221 ctcgacgctg cagcagcgga ttataaggac gatgacgata agtgagcttg atcccccgtc
     2281 gacagctagc tatatcatca atttatgtat tacacataat atcgcactca gtctttcatc
     2341 tacggcaatg taccagctga tataatcagt tattgaaata tttctgaatt taaacttgca
     2401 tcaataaatt tatgtttttg cttggactat aatacctgac ttgttatttt atcaataaat
     2461 atttaaacta tatttctttc aagatcgctg tcatgagacg aattctgaca ggatatattg
     2521 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     2581 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     2641 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     2701 gcagccgtca tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     2761 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     2821 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     2881 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     2941 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     3001 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     3061 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     3121 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     3181 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     3241 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac
     3301 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     3361 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     3421 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     3481 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     3541 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     3601 gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg tgcggctgca tgaaatcctg
     3661 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
     3721 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     3781 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     3841 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
     3901 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     3961 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     4021 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     4081 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     4141 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     4201 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     4261 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     4321 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     4381 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     4441 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     4501 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     4561 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     4621 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
     4681 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     4741 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     4801 gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca
     4861 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca
     4921 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     4981 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     5041 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     5101 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     5161 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     5221 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     5281 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     5341 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     5401 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     5461 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     5521 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     5581 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     5641 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     5701 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     5761 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     5821 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     5881 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
     5941 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
     6001 ggaaaaaggt cgaaaaggac tctttcctgt ggatagcacg tacattggga acccaaagcc
     6061 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
     6121 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
     6181 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
     6241 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
     6301 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
     6361 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
     6421 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     6481 tgacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     6541 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     6601 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     6661 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     6721 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6781 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6841 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6901 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6961 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7021 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7081 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7141 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7201 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7261 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7321 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7381 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7441 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7501 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc
     7561 taggtgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     7621 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     7681 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     7741 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     7801 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     7861 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     7921 gtgattgcgc ctgagcgagt cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     7981 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     8041 caggatattc ttctaatacc tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc
     8101 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     8161 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     8221 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg gtagattgtc gcacctgatt
     8281 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     8341 atcgcggcct tgagcaagac gtttcccgtt gaatatggct cataacagaa cttattattt
     8401 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
     8461 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
     8521 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga
     8581 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
     8641 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
     8701 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
     8761 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctgg
//
